Comparison between enzyme-linked immunosorbent assay and indirect immunofluorescence for detection of antineutrophil cytoplasmic antibodies

Menezes, Julia Miranda; Rossener, Raissa; Silva, Ana Paula Marques Aguirra da; Rodrigues, Silvia Sanches; Mangueira, Cristóvão Luis Pitangueiras

doi:10.31744/einstein_journal/2020ao5132

Cited by 5 publications

(4 citation statements)

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“…Menezes et al found the sensitivity and specificity of ELISA to be 62 and 99%, respectively, in concordance with our findings. 12 However, Harris et al found ELISA to be technically superior to IIF with a specificity of 97% and a positive predictive value of 73%, compared with 90% and 50% of IIF. 15 Similarly, another study also observed superior PPV of ELISA compared with that of ELISA (83 vs. 45%).…”

Section: Discussionmentioning

confidence: 99%

“…5,9 Nonetheless, some researchers expressed the opinion that best results (specificity as high as 99%) could be achieved by combining the results of IIF with that of ELISA (C-ANCA-PR3 ELISA and P-ANCA-MPO-ELISA). [12][13][14] However, employing both the test may not be feasible in resource-limited diagnostic settings. In this study, we tried to assess the diagnostic accuracy of currently available ELISA technique compared with results of IIF.…”

Section: Discussionmentioning

confidence: 99%

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A Comparative Evaluation of Antigen-Specific Sandwich Immunoassay and Indirect Immunofluorescence Assay (IIF) in Detecting Antineutrophil Cytoplasmic Antibodies: Are We Ready to Replace IIF with ELISA as the Primary Screening Method?

et al. 2021

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Background Antineutrophil cytoplasmic antibodies (ANCA) are important biomarkers in the diagnosis of ANCA-associated vasculitis, and indirect immunofluorescence (IIF) had been the method of choice for its detection from the very beginning. However, international consensus on ANCA testing (2017) advocates the use of high-quality immunoassays as the primary screening method. The purpose of this study was to evaluate the diagnostic performance of enzyme-linked immunosorbent assay (ELISA) compared to IIF in detecting ANCA. Methods One-hundred eighty-nine serum samples of suspected or known cases of systemic vasculitis were screened for ANCA by IIF and proteinase-3- and myeloperoxidase-ELISA. In IIF, positive results were further divided into cytoplasmic pattern of ANCA and perinuclear pattern of ANCA, depending upon the pattern of fluorescence. McNemar’s chi-squared test was applied to check the equality of proportions of positive results, and Kappa statistics was used to measure the agreement between the two methods. Diagnostic performance of ELISA was evaluated taking IIF as reference. Results IIF detected ANCA in 17.5% cases and ELISA detected it in 11.6% cases. A good agreement between the overall performance of ELISA and IIF was observed (K-value: 6.8, p-value: < 001). However, a significant difference in the proportion of positive results by the two methods was observed in McNemar’s test (two-sided p-value: 0.007). Taking IIF as standard, ELISA showed 60.6% sensitivity, 98.7% specificity, and predictive value of positive and negative results of 90.9 and 92.2%, respectively. Conclusion The new generation antigen-specific ELISAs had high specificity but the chances of missing cases in primary screening due to the low sensitivity and high false negativity (39.4%) need to be dealt with.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A Comparative Evaluation of Antigen-Specific Sandwich Immunoassay and Indirect Immunofluorescence Assay (IIF) in Detecting Antineutrophil Cytoplasmic Antibodies: Are We Ready to Replace IIF with ELISA as the Primary Screening Method?

et al. 2021

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“…Золотым стандартом выявления АНЦА сегодня считают антиген-специфический ИФА, позволяющий определить содержание ПР3-АНЦА и МПО-АНЦА. Постановка реакции непрямой иммунофлюоресценции (НИФ) перед проведением антиген-специфического ИФА не является обязательным этапом определения АНЦА при подозрении на ААВ [8], хотя необходимо учитывать возможность ложноотрицательных результатов этого метода исследования [24,25]. Соответственно, при отрицательном результате антиген-специфического ИФА могут быть использованы метод НИФ [26], а также ИФА-тесты второго и третьего поколений (capture и anchor) [27].…”

Section: Discussionunclassified

ANCA-positive and ANCA-negative granulomatosis with polyangiitis

Smirnova¹,

Bulanov²,

Zykova³

et al. 2020

CPT

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“…Determination of the patient serum monamines and amino acid neurotransmitters, including dopamine (dopamine, DA), norepinephrine (norepinephrine, NE), 5-serotamine (5-hydroxytruptamine,5-HT) and amino acid neurotransmitters including glutamate (glutamatem, Glu) and glycine (glycine, Gly). Enzymatic-linked immunosorption method was used ( Menezes et al, 2020 ). Patient serum inflammatory factor levels, including interleukin-1 β (interleukin-1 β, IL-1 β), interleukin-6 (interleukin-6, IL-6), and swelling necrosis factor- α (tumor necrosis factor- α, TNF- α)…”

Section: Methodsmentioning

confidence: 99%

Effect of transcranial magnetic stimulation on treatment effect and immune function

Liu

Wang

et al. 2022

Saudi Journal of Biological Sciences

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To explore the effect of transcranial stimulation on the therapeutic effect and immune function of patients with post-stroke depression (PSD). Methods Selection in September 2020–April 2021 on the diagnosis of 70 patients with PSD as the research object, 35 patients were randomly divided into control group and intervention group and control group given conventional treatment, the intervention group in the control group on the basis of the application of transcranial magnetic stimulation treatment, compare the curative effect of two groups of patients after the treatment cycle and the effects on the immune function. Results After treatment, the levels of DA, NE, 5-HT in 2 groups were significantly increased, and those in the observation group were significantly higher than those in the control group (P < 0.05). After 8 weeks of treatment, serum Gly content in 2 groups was significantly increased and Glu content was significantly decreased compared with before treatment. Compared with the control group, serum Gly content in observation group was significantly increased and Glu content was significantly decreased after treatment (P < 0.05). After 8 weeks of treatment, the contents of IL-1β, IL-6 and TNF-α in serum of 2 groups were significantly decreased, compared with the control group, the contents of IL-1β, IL-6 and TNF-α in serum of observation group were significantly decreased (P < 0.05); Before treatment, there was no significant difference in PHQ-9 score and MBI score between the two groups (P > 0.05). After 8 weeks of treatment, PHQ-9 score and MBI score in the two groups were better than before treatment, and the observation group was better than the control group (P < 0.05). Conclusion Transcranial magnetic stimulation therapy can not only effectively promote the synthesis and release of monoamine neurotransmitters in patients with post-stroke depression, regulate the inhibitory/excitatory amino acid neurotransmitters, reduce inflammatory response, improve the clinical treatment effect and enhance the immune function of PSD patients, which has clinical application value.

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Comparison between enzyme-linked immunosorbent assay and indirect immunofluorescence for detection of antineutrophil cytoplasmic antibodies

Abstract: Comparison between enzyme-linked immunosorbent assay and indirect immunofluorescence for detection of antineutrophil cytoplasmic antibodies Comparação entre os métodos de ensaio imunoenzimático e imunoflurescência indireta para a pesquisa de anticorpos anticitoplasma de neutrófilos

Cited by 5 publications

References 15 publications

A Comparative Evaluation of Antigen-Specific Sandwich Immunoassay and Indirect Immunofluorescence Assay (IIF) in Detecting Antineutrophil Cytoplasmic Antibodies: Are We Ready to Replace IIF with ELISA as the Primary Screening Method?

A Comparative Evaluation of Antigen-Specific Sandwich Immunoassay and Indirect Immunofluorescence Assay (IIF) in Detecting Antineutrophil Cytoplasmic Antibodies: Are We Ready to Replace IIF with ELISA as the Primary Screening Method?

ANCA-positive and ANCA-negative granulomatosis with polyangiitis

Effect of transcranial magnetic stimulation on treatment effect and immune function

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