Comparison of acid and amyloglucosidase hydrolysis for estimation of non-structural polysaccharides in feed samples

Abstract

Enzymatic methods (amyloglucosidase) and methods based on acid solutions (0.1, 0.2 and 0.3 M H2SO4 for 1, 2 and 3 h at 100 °C) for the hydrolysis of non‐structural carbohydrates from different feed samples were compared. The monomeric units resulting from the enzymatic and acid hydrolysis were determined by the glucose oxidase and reducing sugar methods. There was a significant effect of acid concentration and of time of hydrolysis on the glucose and reducing sugar values in the hydrolysate. Glucose values were similar for both the amyloglucosidase method and the most intense conditions of hydrolysis (0.3 M for 3 h) for some samples. Under these conditions, however, the reducing sugar values were higher. No acid hydrolysis method was found to estimate correctly the total non‐structural carbohydrates, but α‐linked glucose polymers in biological samples may be determined by sample hydrolysis with a 0.3 M H2SO4 solution for 3 h at 100 °C since the glucose in the hydrolysate is determined by the glucose oxidase method and the sucrose content of the sample is negligible. © 1999 Society of Chemical Industry

Effect of the substitution of urea for soybean meal on digestion in steers

“…The cellulose content of these samples was estimated by the difference between acid detergent fibre and lignin content. Starch content was assayed using amyloglucosidase as previously described (Kozloski et al 1999). Chromium oxide (Cr 2 O 3 ) was determined by sample digestion according to the method of Czarnocki et al (1961) and solubilised chromium was determined by atomic absorption spectrophotometry (Kozloski et al 1998).…”

Evaluation of a High Throughput Starch Analysis Optimised for Wood

“…The optimised analysis was compared to an enzymatic analysis (Sigma STA20 [41] ). We have chosen STA20 because information and reagents are available worldwide, and it uses purified and certified enzymes and therefore does not suffer matrix interference (the alternative acid-based procedures are not suitable for wood matrix analysis [14] , [15] , [16] , [17] , [43] ). Furthermore, we were very familiar with STA20 as we extensively used it as reference in the optimisation phase.…”

“…Hydrolysis of starch has a critical importance in the analysis (an introductory overview of starch analysis is reported in Box 1). Acid hydrolysis is not suitable for wood matrix because the process cleaves structural carbohydrates into monomeric sugars that result in high interference [14] , [15] , [16] , [17] , [18] . Enzymatic hydrolysis can target specific NSC and it has been widely used [17] , [19] , [20] , [21] , [22] , [23] , [24] , [25] , [26] , [27] , but only a few studies have addressed SC of wood [20] , [21] , [26] , [28] .…”

“…Acid hydrolysis is not suitable for wood matrix because the process cleaves structural carbohydrates into monomeric sugars that result in high interference [14] , [15] , [16] , [17] , [18] . Enzymatic hydrolysis can target specific NSC and it has been widely used [17] , [19] , [20] , [21] , [22] , [23] , [24] , [25] , [26] , [27] , but only a few studies have addressed SC of wood [20] , [21] , [26] , [28] . These are subject to technical limitations that include slow purification procedures [22] , long digestion times [20] , [21] , [29] , a neutralization step [23] , the requirement for separate gelatinisation with KOH [26] .…”

Determination of the Depolymerisation Kinetics of Amylose, Amylopectin and Maltodextrin by Aspergillus niger Glucoamylase Using a 2-p-toluidinylnaphthalene-6-sulfonate/Flow-injection Analysis System with Fluorimetric Detection

“…The increased reducing power is generally determined by the Somogyi-Nelson method (Fujii and Kawamura, 1985;Norman, 1982) or the Bernfeld method (Franco et al, 1989;Miranda et al, 1991;Pastrana et al, 1998). Other methodologies are based on the specific determination of the released glucose, after a given incubation time, using the glucose oxidase-peroxidase method (Abe et al, 1988;Kozloski et al, 1999;). An alternative methodology for the determination of amylolitic activities, when acting on their natural substrates, is the use of a flow-injection analysis (FIA) system with 2-p-toluidinylnaphthalene-6-sulfonate (2,6-TNS) as the fluorescent probe and fluorimetric detection.…”