Comparison of Curet and Paper Point Sampling of Subgingival Bacteria as Analyzed by Real‐Time Polymerase Chain Reaction

Jervøe‐Storm, Pia‐Merete; AlAhdab, Hazem; Koltzscher, Max; Fimmers, Rolf; Jepsen, Søren

doi:10.1902/jop.2007.060218

Cited by 67 publications

(64 citation statements)

References 17 publications

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“…A possible explanation for these findings may be the absorbent properties of the PP making them more prone to absorb crevicular fluid, desquamated cells, lysed leukocytes and human DNA debris. Outcomes of the present study are in line with those reported by Jervøe-Storm et al (2007), who evaluated by qPCR the amount of bacterial DNA in samples collected by PP and curettes from sites with PD N 5 mm in ChP subjects. The authors showed that curettes recovered significantly more total bacteria DNA than PP.…”

supporting

confidence: 89%

Evaluation of human and microbial DNA content in subgingival plaque samples collected by paper points or curette

Pérez-Chaparro

Duarte

Pannuti

et al. 2015

Journal of Microbiological Methods

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supporting

confidence: 89%

Evaluation of human and microbial DNA content in subgingival plaque samples collected by paper points or curette

Pérez-Chaparro

Duarte

Pannuti

et al. 2015

Journal of Microbiological Methods

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“…However, the present findings contradict those of studies reporting a positive correlation of number of P. gingivalis with PPD and PAL (39,40). There are a number of possible explanations for this inconsistency, including differences in disease status at the sampled site and the method used to detect and estimate the copy numbers of pathogens present (41). Loomer reported that analysis of the overall oral microbiota profile of a patient is more representative when a greater number of sites is sampled (42).…”

Section: Discussioncontrasting

confidence: 97%

Comparison of nonsurgical periodontal therapy with oral hygiene instruction alone for chronic periodontitis

et al. 2017

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We evaluated changes in clinical variablesand microbiological profiles of periodontopathogens among 56 patients with moderate to severe CP who were randomly assigned to oral hygiene instruction (OHI; n = 28) or nonsurgical periodontal treatment (NSPT; n = 28). Periodontal variables were assessed and subgingival plaque samples were obtained from deep pockets (≥5 mm) at baseline and 3 months after treatment. Real-time polymerase chain reaction was used to quantify Actinobacillus actinomycetemcomitans, Tannerella forsythia, Porphyromonas gingivalis, and Prevotella intermedia. All clinical variables significantly improved in both groups. Improvements in gingival bleeding index (GBI), probing pocket depth (PPD), and periodontal attachment loss (PAL) were significantly greater at 3 months after treatment in the NSPT group. At baseline, the prevalences of all pathogens were high. Significant reductions in microbial count were observed for A. actinomycetemcomitans and T. forsythia (P ≤ 0.05) in the NSPT group. None of the improvements in clinical variables was associated with changes in microbiological profiles. At 3 months after treatment, NSPT was associated with significantly greater improvements in GBI, PPD, and PAL as compared with OHI. A. actinomycetemcomitans and T. forsythia counts were significantly lower in the NSPT group.

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“…In comparison to paper points, curettes collect plaque from the entire pocket lining. [17] Polymerase chain reaction (PCR) based methods are more specific and sensitive as they are based on detection of gene specific DNA sequences. Hence, it represents a valuable method to determine the viral load in diseased and healthy sites.…”

Section: International Journal Of Dental Researchmentioning

confidence: 99%

Role of herpes simplex-1, epstein barr and human cytomegalo viruses in aggressive periodontitis

Dani¹,

Dwarakanath

Alampalli

et al. 2013

International Journal of Dental Research

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Objectives: Aggressive periodontitis is characterized rapid and severe destruction of the tooth supporting tissues with a complex and unclear etiopathogenesis. The present study was designed to identify the role of herpesviruses in pathogenesis of aggressive periodontitis. Material and methods: Study included 15 subjects diagnosed with aggressive periodontitis (group A) and 15 periodontally healthy subjects (group B). Subgingival plaque was collected from the deepest periodontal pocket and gingival tissue biopsy from the adjacent interdental papilla. Results analyzed by polymerase chain reaction. Results: EBV detected in 67% plaque and 73% tissue samples in group A, 7% plaque and none in the tissue samples in group B. HCMV identified in 53% plaque and 20% of tissue samples in group A, 20% plaque and 7% tissue samples in group B. HSV-1 found in 47% plaque and 13% tissue samples in group A, 13% plaque and 47% tissue samples in group B. Conclusion: An increased prevalence of EBV, HCMV and HSV-1 in group A in comparison to healthy controls was observed. Only EBV showed significant difference between both groups. Despite similarity in pathogenic traits between herpesvirus diseases and Periodontitis, delineating the exact role that viruses play in the etiopathogenesis of aggressive periodontitis is difficult.

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Comparison of Curet and Paper Point Sampling of Subgingival Bacteria as Analyzed by Real‐Time Polymerase Chain Reaction

Abstract: Overall, there was a relatively good agreement for the results of paper point and curet sampling. Thus, both techniques seem to be suitable for microbiological diagnostics.

Cited by 67 publications

References 17 publications

Evaluation of human and microbial DNA content in subgingival plaque samples collected by paper points or curette

Evaluation of human and microbial DNA content in subgingival plaque samples collected by paper points or curette

Comparison of nonsurgical periodontal therapy with oral hygiene instruction alone for chronic periodontitis

Role of herpes simplex-1, epstein barr and human cytomegalo viruses in aggressive periodontitis

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