Comparison of ELISA and PCR vis-à-vis cultural methods for detecting Aeromonas spp. in foods of animal origin

Electrochemical detection of foodborne pathogen Aeromonas hydrophila by DNA hybridization biosensor

“…doi:10.1016/j.bios.2010.08.030 classical methods of effective control of the pathogen in food still require development (EPA, 2006). The procedures based on the use of immunoassays or polymerase chain reaction (PCR) was already proposed (Kingombe et al, 2004;Arora et al, 2006). The nucleic acid-based detection of A. hydrophila a DNA piezoelectric biosensor was also presented (Tombelli et al, 2000).…”

Detection of toxigenic strains of Aeromonas species in foods by a multiplex PCR assay

“…Numerous methods, both the conventional as well as rapid systems including PCRs have been tried for the detection of foodborne pathogens [13,16,18]. However, in the case of Aeromonas, the PCR has mostly been used for the characterization of isolates either for their virulence properties or for the phylogenetic position [11,23].…”

“…Several virulence-associated genes have been targeted for the detection of potentially pathogenic aeromonads by PCR assays that include either cytolysin or hemolysin or both of these genes [11][12][13][14][15]. Recently Chu and Lu [14] reported a multiplex PCR (mPCR) to rapidly and specifi cally detect pathogenic A. hydrophila from symptomatic and asymptomatic fi sh targeting aerolysin and 16S rDNA genes.…”

Prevalence of different outer membrane proteins in isolates of Aeromonas species

“…An Omp based indirect plate ELISA and a duplex-PCR were developed for the detection of Aeromonas from food sample (Arora et al 2006). This study is a continuation of our earlier work where we reported OmpTS to be a good vaccine candidate (Khushiramani et al 2007a, b).…”