Comparison of seed and vegetative propagation methods for red squill

Nodules of Charybdis numidica maintained in liquid Murashige and Skoog (MS) medium with 20 lmol BA in the dark were subjected to different treatments under continuous light for shoot regeneration. A high regeneration rate without hyperhydration of the shoots was observed on semisolid basal MS medium with 1% sucrose. The use of liquid MS medium (1% sucrose, no growth regulators) resulted in a significantly lower amount of shoots per gramme of nodules under both submerged and temporary immersion (TI) conditions. Shoot hyperhydration was lowest in a TI system with one 5 min immersion every 24 h. When compared on a per container base, large amounts of shoots could be produced in the TI system with less labour input than in the system with semisolid medium.

show abstract

“…However, the vegetative multiplication by bulb offsets is very low (van Horn and Domingo, 1950). In vitro propagation of Charybdis sp.…”

Section: Introductionmentioning

confidence: 99%

Shoot regeneration from nodules of Charybdis sp.: a comparison of semisolid, liquid and temporary immersion culture systems

Wawrosch

Kongbangkerd

K�pf³

et al. 2005

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

Shoot regeneration from nodules of Charybdis sp.: A comparison of semisolid, liquid and temporary immersion culture systems

Wawrosch

Kongbangkerd

Köpf

et al.

Liquid Culture Systems for in Vitro Plant Propagation

View full text Add to dashboard Cite

In vitro propagation of red squill, Urginea maritima Baker

Grari

Backhaus

1987

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Red squill (Urginea maritima Baker), a plant in the Liliaceae of potential use as a rodenticide, was successfully propagated in vitro. Bulblets were induced from red squill bulb-scales cultured in the dark on a medium containing Murashige and Skoog [6] salts supplemented with a combination of 0.5 or 1.6 #M naphthaleneacetic acid and 0.4 or 1.3/~M 6-benzylaminopurine. Bulblets induced in vitro were rooted in medium containing 0.5 or 1.6/tM naphthaleneacetic acid and planted in vermiculite. Bulb-scale explants could be returned to fresh medium regenerate new bulblets, which were easily rooted. Alternatively. bulblets could be subcultured to regenerate adventitious shoots in medium containing 4.4 or 13.2 pM 6-benzylaminopurine. Shoots regenerated in this manner proved difficult to root by a number of treatments tested. Regenerated bulblets were chilled at 5 C for 3 to 4 weeks to induce leaf emergence prior to transfer to the greenhouse.

show abstract

Comparison of seed and vegetative propagation methods for red squill

Cited by 5 publications

References 1 publication

Shoot regeneration from nodules of Charybdis sp.: a comparison of semisolid, liquid and temporary immersion culture systems

Shoot regeneration from nodules of Charybdis sp.: a comparison of semisolid, liquid and temporary immersion culture systems

Shoot regeneration from nodules of Charybdis sp.: A comparison of semisolid, liquid and temporary immersion culture systems

In vitro propagation of red squill, Urginea maritima Baker

Contact Info

Product

Resources

About