Comparison of the cytotoxic and inflammatory responses of titanium particles with different methods for endotoxin removal in RAW264.7 macrophages

Ding, Huifeng; Zhu, Zhenan; Tang, Tingting; Yu, Degang; Yu, Bo; Dai, Kerong

doi:10.1007/s10856-012-4574-x

Cited by 18 publications

(18 citation statements)

References 45 publications

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“…A previous study showed that cytokine production and osteoclast differentiation are stimulated by endotoxins that adhere to wear particles [34] . Furthermore, LPS treatment of titanium particles increased TNF-α and IL-1β production in THP-1 cells [35] .…”

Section: Discussionmentioning

confidence: 99%

Potential proinflammatory and osteogenic effects of dicalcium silicate particles in vitro

Chen¹,

Zhu²,

Liu³

et al. 2015

Journal of the Mechanical Behavior of Biomedical Materials

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Section: Discussionmentioning

confidence: 99%

Potential proinflammatory and osteogenic effects of dicalcium silicate particles in vitro

Chen¹,

Zhu²,

Liu³

et al. 2015

Journal of the Mechanical Behavior of Biomedical Materials

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“…Wear particles have been known to have high affinity for endotoxin [70,71] which may allow systemic endotoxin to bind to generated wear particles. Furthermore, endotoxin in subclinical concentrations of bacteria have been demonstrated to be present on implants and wear particles [43].…”

Section: Discussionmentioning

confidence: 99%

Integrin-directed modulation of macrophage responses to biomaterials

et al. 2014

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Macrophages are the primary mediator of chronic inflammatory responses to implanted biomaterials, in cases when the material is either in particulate or bulk form. Chronic inflammation limits the performance and functional life of numerous implanted medical devices, and modulating macrophage interactions with biomaterials to mitigate this response would be beneficial. The integrin family of cell surface receptors mediates cell adhesion through binding to adhesive proteins nonspecifically adsorbed onto biomaterial surfaces. In this work, the roles of integrin Mac-1 (αMβ2) and RGD-binding integrins were investigated using model systems for both particulate and bulk biomaterials. Specifically, the macrophage functions of phagocytosis and inflammatory cytokine secretion in response to a model particulate material, polystyrene microparticles were investigated. Opsonizing proteins modulated microparticle uptake, and integrin Mac-1 and RGD-binding integrins were found to control microparticle uptake in an opsonin-dependent manner. The presence of adsorbed endotoxin did not affect microparticle uptake levels, but was required for the production of inflammatory cytokines in response to microparticles. Furthermore, it was demonstrated that integrin Mac-1 and RGD-binding integrins influence the in vivo foreign body response to a bulk biomaterial, subcutaneously implanted polyethylene terephthalate. A thinner foreign body capsule was formed when integrin Mac-1 was absent (~30% thinner) or when RGD-binding integrins were blocked by controlled release of a blocking peptide (~45% thinner). These findings indicate integrin Mac-1 and RGD-binding integrins are involved and may serve as therapeutic targets to mitigate macrophage inflammatory responses to both particulate and bulk biomaterials.

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“…Previous studies have shown that cytokine production and osteoclast differentiation are stimulated by adherent endotoxins on wear particles [37]. Therefore, our discs were disinfected using autoclave and tested for endotoxins to ensure that the discs were free of contamination.…”

Section: Discussionmentioning

confidence: 99%

Exposure of the murine RAW 264.7 macrophage cell line to dicalcium silicate coating: assessment of cytotoxicity and pro-inflammatory effects

Chen

Liu

Wei

et al. 2016

J Mater Sci: Mater Med

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Inflammatory effects are significant elements of the immune response to biomaterials. Previously, we reported inflammatory effects in response to dicalcium silicate (Ca2SiO4, C2S) particles. However, the immunological effects of C2S coatings have not been studied. C2S often used as coatings materials in orthopedic and dentistry applications. It may have different effect from C2S particles. Further, it remains unclear whether C2S coating is equally biocompatible as 45S5 coating. The aim of this study was to test the cytotoxicity and pro-inflammatory effects of C2S coating on RAW 264.7 macrophages. C2S and 45S5 coatings were characterized using scanning electron microscopy (SEM), atomic force microscopy (AFM), energy dispersive analysis (EDS) and X-ray diffraction (XRD). inductively coupled plasma optical emission spectroscopy (ICP-OES) was used to detect ionic concentrations after soaking coated discs in medium. The cytotoxicity of C2S and 45S5 coatings against RAW 264.7 macrophages was measured using the LDH Cytotoxicity Assay Kit, Cell Counting Kit-8 (CCK-8) assays and flow cytometry for apoptosis assays. The gene and protein expression of TNF-α, IL-6 and IL-1β were detected using RT-q PCR and ELISA, respectively. The tested coating materials are not cytotoxic to macrophages. The C2S-coated surface stimulated macrophages to express pro-inflammatory mediators, such as TNF-α, IL-6 and IL-1β, and C2S coating caused less IL-6 but greater IL-1β production than the 45S5 coating. C2S coating have no cytotoxicity when directly cultured with macrophages. C2S and 45S5 coatings both have the potential to induce pro-inflammatory effects, and the biocompatibility of C2S is similar to that of 45S5.

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Comparison of the cytotoxic and inflammatory responses of titanium particles with different methods for endotoxin removal in RAW264.7 macrophages

Cited by 18 publications

References 45 publications

Potential proinflammatory and osteogenic effects of dicalcium silicate particles in vitro

Potential proinflammatory and osteogenic effects of dicalcium silicate particles in vitro

Integrin-directed modulation of macrophage responses to biomaterials

Exposure of the murine RAW 264.7 macrophage cell line to dicalcium silicate coating: assessment of cytotoxicity and pro-inflammatory effects

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