Comparison of the Effects of the 1975 Japanese Diet and the Modern Mediterranean Diet on Lipid Metabolism in Mice

Mizowaki, Yui; Sugawara, Saeko; Yamamoto, Kazushi; Sakamoto, Yusuke; Iwagaki, Yui; Kawakami, Yuki; Igarashi, Miki; Tsuduki, Tsuyoshi

doi:10.5650/jos.ess16241

Cited by 18 publications

(13 citation statements)

References 39 publications

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“…To determine the nutritional composition of the test diets, each of the test foods was freeze‐dried in a vacuum freeze dryer (FD‐550 R; Tokyo Rikakikai, Tokyo, Japan), and then a grinder‐mixer was used to homogenize them. Next, the actual values of the nutritional components (fat, protein, moisture, ash, carbohydrate, and energy) were measured as previously described . The amount of fat was measured using the acid hydrolysis method, and protein levels were measured using the Duma method (Sumigraph NC Analyzer NC‐220 F; Sumika Chemical Analysis Service, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

Abdominal Fat in Individuals with Overweight Reduced by Consumption of a 1975 Japanese Diet: A Randomized Controlled Trial

Asano

Kushida

Yamamoto

et al. 2019

Obesity

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Objective: This study aimed to investigate whether the intake of the 1975 Japanese diet (JD) could reduce the amount of abdominal fat in people with overweight. Methods: Using a single-blind randomized controlled trial, the modern diet (MD) was compared with the 1975-type JD, which is based on the MD but includes five characteristics of the 1975 JD in an enhanced form. Overweight people were randomly assigned to an MD group (n = 30) and a JD group (n = 30). The participants consumed test diets that were provided three times a day for 28 days. Body composition measurements and blood biochemical examinations were performed before and after the test diet intake, and the proportions of change were compared. Results: Those in the JD group had significantly decreased BMI, fat mass, and levels of low-density lipoprotein cholesterol, glycated hemoglobin, and C-reactive protein (P = 0.002, 0.015, 0.014, 0.012, and 0.039, respectively) and significantly increased high-density lipoprotein cholesterol levels compared with those in the MD group (P = 0.020). Conclusions: The intake of a diet with the characteristics of the 1975 JD may have beneficial effects on lipid metabolism in people with overweight and reduce the onset risk of metabolism-related disorders, such as obesity and diabetes. Abdominal Fat and 1975 Japanese Diet Asano et al.

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Section: Methodsmentioning

confidence: 99%

Abdominal Fat in Individuals with Overweight Reduced by Consumption of a 1975 Japanese Diet: A Randomized Controlled Trial

Asano

Kushida

Yamamoto

et al. 2019

Obesity

Self Cite

View full text Add to dashboard Cite

show abstract

“…Serum insulin was determined using an ELISA kit (Morinaga Institute of Biological Science, Yokohama, Japan). Homeostatic model assessment of insulin resistance (HOMA‐IR) was calculated from the glucose and insulin levels . Serum and liver thiobarbituric acid reactive substances (TBARS) were measured as described previously .…”

Section: Methodsmentioning

confidence: 99%

“…For histological analysis, liver and epididymal adipose tissue were fixed in 10% formalin and embedded in paraffin . Vertical sections (5 μm) were cut, mounted on a glass slide, stained with hematoxylin and eosin (HE), and observed using a microscope (BZ‐9000; Keyence, Osaka, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…Vertical sections (5 μm) were cut, mounted on a glass slide, stained with hematoxylin and eosin (HE), and observed using a microscope (BZ‐9000; Keyence, Osaka, Japan). The mean area of adipocytes was calculated in each group …”

Section: Methodsmentioning

confidence: 99%

“…For real‐time quantitative reverse transcriptase PCR (qRT‐PCR), total RNA was isolated from liver using an RNeasy Mini Kit (Qiagen, Valencia, CA, USA), eluted with 40 μL of RNase‐free water, and stored at −80 °C until use. To quantify expression levels of genes, the mRNA levels for actin beta, cytoplasmic ( Actb ), acyl‐coenzyme A oxidase 1, palmitoyl ( Aco ), carnitine palmitoyl transferase 1β ( Cpt1 ), carnitine palmitoyltransferase 2 ( Cpt2 ), fatty acid synthase ( Fas ), glucose‐6‐phosphate dehydrogenase X‐linked ( G6pdx ), malic enzyme ( Me ), peroxisome proliferator‐activated receptor alpha ( Pparα ), and sterol regulatory element‐binding protein/factor 1 ( Srebp‐1c ) in liver were determined with a Thermal Cycler Dice Real Time System (Takara Bio, Otsu, Japan).…”

Section: Methodsmentioning

confidence: 99%

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