Complete genome sequence and structural characterization of a novel iflavirus isolated from Opsiphanes invirae (Lepidoptera: Nymphalidae)

Silva, Leonardo A.; Ardisson-Araújo, Daniel Mendes Pereira; Tinôco, Ricardo Salles; Melo, Fernando Lucas; Ribeiro, Bergmann Morais

doi:10.1016/j.jip.2015.08.001

Cited by 21 publications

(16 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Herein, a novel virus named H. armigera iflavirus ( Carrillo-Tripp et al, 2014;Silva et al, 2015;van Oers, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…(1) non-enveloped icosahedral particles measuring 30 nm in diameter; (2) genome translation into a polyprotein; (3) the viral coat proteins containing three jelly-roll domains; (4) a three-domain containing a superfamily III helicase, a (cysteine) proteinase with a chymotrypsin-like fold and an RNA-dependent RNA polymerase (RdRp) (Le Gall et al, 2008 (Lanzi et al, 2006), Ectropis obliqua virus , Infectious flacherie virus (Isawa et al, 1998), Lygus lineolaris virus 1 (Perera et al, 2012), Nilaparvata lugens honeydew virus 1 (Murakami et al, 2013), Perina nuda virus (Wu et al, 2002), Sacbrood virus (Ghosh et al, 1999), Slow bee paralysis virus (de Miranda et al, 2010), and Varroa destructor virus 1 (Ongus et al, 2004), although other iflaviruses have been reported (Silva et al, 2015;Suzuki et al, 2015). Herein, we report the nucleotide sequence, genome organization, phylogeny, transmission and tissue distribution of HaIV.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of a novel member of genus Iflavirus in Helicoverpa armigera

Yang

et al. 2017

Journal of Invertebrate Pathology

View full text Add to dashboard Cite

The cotton bollworm, Helicoverpa armigera, is one of the most important agricultural pests of many economic crops worldwide. Herein, we found a novel single-strand RNA virus by RNA-Seq and Polymerase Chain Reaction (PCR) method in H. armigera named Helicoverpa armigera iflavirus (HaIV), which possessed a genome with 10,017 nucleotides in length and contained a single large open reading frame (ORF) encoding a putative polyprotein of 3,021 amino acids with a predicted molecular mass of 344.16 kDa and a theoretical isoelectric point (pI) of 6.45. The deduced amino acid sequence showed highest similarity (61.0%) with the protein of Lymantria dispar Iflavirus 1. Phylogenetic analysis with putative RdRp amino acid sequences indicated that the virus clustered with members of the genus Iflavirus. The virus was mainly distributed in the fat body of its host and was found to be capable of both horizontal and vertical transmission. The efficiency of perorally horizontal transmission was dose dependent (100% infection rate with a viral dose of 10 8 copies /μl) while vertical transmission efficiency was found to be relatively low (< 28.57%).These results suggest that we have found a novel member of genus Iflavirus in H.armigera.

show abstract

“…Herein, a novel virus named H. armigera iflavirus ( Carrillo-Tripp et al, 2014;Silva et al, 2015;van Oers, 2010).…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of a novel member of genus Iflavirus in Helicoverpa armigera

Yang

et al. 2017

Journal of Invertebrate Pathology

View full text Add to dashboard Cite

show abstract

“…We used TEM to directly confirm the presence of virus particles, first in a sample purified from the in vitro supernatant ( Figure 3B). Iflavirus capsids have been reported to have a diameter of around 30 nanometers (Silva et al, 2015) , and the regular size and shape of viral particles help them stand out by TEM. Indeed, a uranyl acetate negative stain of viral particles collected by ultracentrifugation from the extracellular fraction showed an abundance of symmetric ~30 nm objects, consistent with our expectations for the iflaviridae (Fig 3B).…”

Section: Evidence Of Intracellular and Extracellular Virus In E Muscmentioning

confidence: 99%

Entomophthovirus: An insect-derived iflavirus that infects a behavior manipulating fungal pathogen of dipterans

Bronski

et al. 2018

Preprint

View full text Add to dashboard Cite

We discovered a virus infecting Entomophthora muscae , a behavior-manipulating fungal pathogen of dipterans. The virus, which we name Entomophthovirus, is a capsid-forming, positive-strand RNA virus in the viral family iflaviridae, whose known members almost exclusively infect insects. We show that the virus RNA is expressed at high levels in fungal cells in vitro and during in vivo infections of Drosophila melanogaster , and that virus particles are present in E. muscae . Two close relatives of the virus had been previously described as insect viruses based on the presence of viral genomes in transcriptomes assembled from RNA extracted from wild dipterans. By analyzing sequencing data from these earlier reports, we show that both dipteran samples were co-infected with E. muscae . We also find the virus in RNA sequencing data from samples of two other species of dipterans, Musca domestica and Delia radicum , known to be infected with E. muscae . These data establish that Entomophthovirus is widely, and seemingly obligately, associated with E. muscae . As other members of the iflaviridae cause behavioral changes in insects, we speculate on the possibility that Entomophthovirus plays a role in E. muscae involved host manipulation.

show abstract

“…The iflavirus infection process is still not well understood and seems to differ depending on the host type. In some iflavirus hosts, such as the silkworm Bombyx mori (Himeno et al, 1974), the oil palm tree pest Opsiphanes invirae (Silva et al, 2015), and the honey bee Apis mellifera (Yue et al, 2007), signs of infection are quite evident depending on the virus species and host condition. For example, in silkworm larvae, the pathology of infectious flacherie virus (the type species of iflavirus) infection includes a brown color or other discoloration of the posterior and middle parts of the insect body and flaccidity (Inoue and Ayuzawa, 1972).…”

Section: Introductionmentioning

confidence: 99%

An iflavirus found in stink bugs (Hemiptera: Pentatomidae) of four different species

et al. 2019

Self Cite

View full text Add to dashboard Cite

An analysis of transcriptomes from the antennae of the three South American stink bugs (Euschistus heros, Chinavia ubica, and Dichelops melacanthus) revealed the presence of picorna-like virus genome-length RNAs with high sequence identity to the genome of Halyomorpha halys virus (HhV), originally discovered in the transcriptome of the brown marmorated stink bug, Halyomorpha halys. Features of the genome, phylogenetic relationships to other viruses, and the appearances of virus-like particles isolated from host stink bugs all confirm that these viruses are iflaviruses and isolates of an undescribed species. Iflavirus RNAs were present at high levels (40%-90% of transcriptome reads) in the stink bug antennal transcriptomes. In whole-insect transcriptomes of H. halys, HhV reads were > 500-fold more abundant in adults than in nymphs. We identified from field population a subject of species E. heros infected by this iflavirus. The results of the analysis suggest that these iflaviruses are able to produce large quantities of their RNAs without causing any obvious pathology to their hosts.

show abstract

Complete genome sequence and structural characterization of a novel iflavirus isolated from Opsiphanes invirae (Lepidoptera: Nymphalidae)

Cited by 21 publications

References 35 publications

Characterization of a novel member of genus Iflavirus in Helicoverpa armigera

Characterization of a novel member of genus Iflavirus in Helicoverpa armigera

Entomophthovirus: An insect-derived iflavirus that infects a behavior manipulating fungal pathogen of dipterans

An iflavirus found in stink bugs (Hemiptera: Pentatomidae) of four different species

Contact Info

Product

Resources

About