Complete nucleotide sequences of a distinct bipartite begomovirus, bitter gourd yellow vein virus, infecting Momordica charantia

Abstract

Momordica charantia (Cucurbitaceae), a vegetable crop commonly cultivated throughout Pakistan, and begomoviruses, a serious threat to crop plants, are natives of tropical and subtropical regions of the world. Leaf samples of M. charantia with yellow vein symptoms typical of begomovirus infections and samples from apparently healthy plants were collected from areas around Lahore in 2004. Full-length clones of a bipartite begomovirus were isolated from symptomatic samples. The complete nucleotide sequences of the components of one isolate were determined, and these showed the arrangement of genes typical of Old World begomoviruses. The complete nucleotides sequence of DNA A showed the highest nucleotide sequence identity (86.9%) to an isolate of Tomato leaf curl New Delhi virus (ToLCNDV), confirming it to belong to a distinct species of begomovirus, for which the name Bitter gourd yellow vein virus (BGYVV) is proposed. Sequence comparisons showed that BGYVV likely emerged as a result of inter-specific recombination between ToLCNDV and tomato leaf curl Bangladesh virus (ToLCBDV). The complete nucleotide sequence of DNA B showed 97.2% nucleotide sequence identity to that of an Indian strain of Squash leaf curl China virus.

Investigation on disease incidence, distribution and diversity of Begomoviruses infecting cucurbitaceous crops in North Bengal, India

“…Present isolate BGYVV also formed group, showing closeness to Indian and Bangladesh isolates. Bitter gourd yellow vein virus (BGYVV) have been reported from several countries like Sri Lanka (Bandaranayake et al, 2014), Pakistan (Tahir et al, 2010a) and India (Mohanan and Sharma, 2020). Sequence comparisons revealed that the inter-specific recombination between Tomato leaf curl New Delhi virus and Tomato leaf curl PCR of the total DNA extracted from 153 samples were done.…”

Ageratum enation virus—A Begomovirus of Weeds with the Potential to Infect Crops

“…For isolates SOL and ACL primer pair WTGF/WTGR [ 20 ] were used to PCR amplify an approximately 1500 bp fragment spanning all of the intergenic region and most of the Rep gene (data not shown). A specific pair of abutting primers BGAF/BGAR [ 21 ] containing a unique restriction enzyme ( Apa 1) site, to PCR-amplify the complete begomovirus genome. The full-length begomovirus from isolate ABF was PCR-amplified with primer pair BF/BR [ 22 ].…”

Molecular characterization of a first begomovirus associated with lentil (Lens culinaris) from India

“…The ORFs AC4 and AC5 however, presented some interesting comparisons. The AC4 ORF was 294 nt long and coded a putative protein of 97 aa similar to that in ToLCNDV (FN645905) and BGYVV (KJ862841), but it was only 177 nt long and encoded a product of 58 aa in all other viruses used for comparison (data not shown) except BGYVV (AM491590) in which it is truncated being only 126 nt long and encoding a protein with only 41 aa (Tahir et al, 2010).…”

“…Ln1A has 96.3% similarity with another sequence KJ862841, available in data base under the name of BGYVV from Bangladesh. Interestingly, Tahir et al (2010) while analyzing the complete nt sequence of DNA-A (AM491590) of the begomovirus infecting bitter gourd (Momordica charantia), found it to have 94.4% similarity with ToLCNDV (FN645905) and named it along with latter as BGYVV. The existence of BGYVV as a distinct begomovirus species has however been questioned and deleted from the list of begomovirus species since it had 91% nt identity with ToLCNDV (AM747291) (http://talk.ictvonline.org/files/proposals/taxonomy_pro-posals_plant1/m/plant01/5307.aspx).…”

“…Revision of begomovirus species demarcation criterion (Brown et al, 2015) has resulted in the regrouping/renaming of many isolates of different species. BGYVV (Tahir et al, 2010) is one such begomovirus species which Brown et al (2015) based on their extensive analysis using different statistical tools, have shown it to be an isolate of ToLCNDV rather than a new begomovirus species. This is the first report of the association of a begomovirus with lentil from India.…”