Complexity of microRNA function and the role of isomiRs in lipid homeostasis

Vickers, Kasey C.; Sethupathy, Praveen; Baran-Gale, Jeanette; Remaley, Alan T.

doi:10.1194/jlr.r034801

Cited by 48 publications

(34 citation statements)

References 140 publications

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“…3b). This finding may reflect the stability of TrkC-miR1-5p versus its -3p counterpart which is consistent with similar report [42]. Relatively higher expression of TrkC-miR1 in glioma samples candidate it as a diagnostic marker against other brain tumors upon confirmation of its statistical significance.…”

Section: Detection Of Endogenous Trkc-mir1 In Human Tissues and Cell supporting

confidence: 91%

Experimental verification of a conserved intronic microRNA located in the human TrkC gene with a cell type-dependent apoptotic function

Dokanehiifard

Soltani

Parsi

et al. 2015

Cell. Mol. Life Sci.

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Tropomyosin receptor kinase C (TrkC) is involved in cell survival, apoptosis induction and tumorigenesis. We hypothesized that, similar to p75(NTR) receptor, some of the diverse functions of TrkC could be mediated by a microRNA (miRNA) embedded within the gene. Here, we experimentally verified the expression and processing of two bioinformatically predicted miRNAs named TrkC-miR1-5p and TrkC-miR1-3p. Transfecting a DNA fragment corresponding to the TrkC-premir1 sequence in HEK293t cells caused ~300-fold elevation in the level of mature TrkC-miR1 and also a significant downregulation of its predicted target genes. Furthermore, endogenous TrkC-miR1 was detected in several cell lines and brain tumors confirming its endogenous generation. Furthermore, its orthologous miRNA was detected in developing rat brain. Accordingly, TrkC-miR1 expression was increased during the course of neural differentiation of NT2 cell, whereas its suppression attenuated NT2 differentiation. Consistent with opposite functions of TrkC, TrkC-miR1 overexpression promoted survival and apoptosis in U87 and HEK293t cell lines, respectively. In conclusion, our data report the discovery of a new miRNA with overlapping function to TrkC.

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Section: Detection Of Endogenous Trkc-mir1 In Human Tissues and Cell supporting

confidence: 91%

Experimental verification of a conserved intronic microRNA located in the human TrkC gene with a cell type-dependent apoptotic function

Dokanehiifard

Soltani

Parsi

et al. 2015

Cell. Mol. Life Sci.

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“…This heterogeneity could lead to differential target repression and distinct biological activities. IsomiR preference can vary by cell type and isomiR switching has been detected in disease[52, 55, 56]. The molecular mechanism for isomiR switching is unclear.…”

Section: The Canonical Mirna Biogenesis Pathwaymentioning

confidence: 99%

An overview of microRNAs

Hammond

2015

Advanced Drug Delivery Reviews

1,184

886

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The discovery of the first microRNA (miRNA) over 20 years ago has ushered in a new era in molecular biology. There are now over 2000 miRNAs that have been discovered in humans and it is believed that they collectively regulate one third of the genes in the genome. miRNAs have been linked to many human diseases and are being pursued as clinical diagnostics and as therapeutic targets. This review presents an overview of the miRNA pathway, including biogenesis routes, biological roles, and clinical approaches.

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“…Molecular regulation of miRNAs & potential biomarkers in the progression of hepatic steatosis to NASH Review the new function of miR-33, including miR-33a and miR-33b over lipid metabolism and fatty acid oxidation through regulation of sterol regulatory elementbinding protein. miR-33 is a family of miRNA precursors, which are processed by the Dicer enzyme to give mature miRNAs and have recently been shown to regulate lipid homeostasis in concert with their host genes [32,33]. The sequence of miR-33 is identical, and the stem-loop of the pre-miRNA is highly conserved in mammals and regulate liver steatosis through its target of Sterol regulatory element-binding transcription factor (SREBP) [34,35].…”

Section: General Progress Of Nafldmentioning

confidence: 99%

Molecular Regulation of Mirnas and Potential Biomarkers in the Progression of Hepatic Steatosis to NASH

et al. 2015

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Increasing evidence suggests that microRNAs regulate diverse biological functions in the liver and play a very important function in metabolic-related disorders such as nonalcoholic fatty liver disease via regulating their target genes expression. In this review, we summarized the most recent progress in identification of miRNAs involving in the progression of liver steatosis and discussed the possible mechanisms by which miRNAs contribute to the diverse pathogenic liver injuries. We provide insights into the functional network of miRNAs by connecting miRNAs, their targets and biological pathways associated to hepatic steatosis and fibrosis, with important implications for our understanding of phenotypic-based disease pathogenesis. We also discuss the possible roles and challenges of miRNAs as biomarkers for drug-induced liver injury.

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Complexity of microRNA function and the role of isomiRs in lipid homeostasis

Cited by 48 publications

References 140 publications

Experimental verification of a conserved intronic microRNA located in the human TrkC gene with a cell type-dependent apoptotic function

Experimental verification of a conserved intronic microRNA located in the human TrkC gene with a cell type-dependent apoptotic function

An overview of microRNAs

Molecular Regulation of Mirnas and Potential Biomarkers in the Progression of Hepatic Steatosis to NASH

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