Complexity of the cold acclimation response in Drosophila melanogaster

Rako, Lea; Hoffmann, Ary A.

doi:10.1016/j.jinsphys.2005.09.007

Cited by 147 publications

(161 citation statements)

References 31 publications

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“…Such variation has also been described by other authors (e.g. Tucic, 1979;Rako and Hoffmann, 2006;Jensen et al, 2007), and may be of similar origin. Heino and Lumme (1989) show that there is variation in low temperature tolerance between sexes of adult D. virilis and D. lummei at the same age.…”

Section: Discussionsupporting

confidence: 85%

“…To our knowledge, this is the first study to show that RCH applies to both acute and chronic cold exposure, in spite of the postulated differences between the damage caused by these two stressors (Sinclair and Roberts, 2005;Rako and Hoffmann, 2006). Acute cold injury is hypothesised to be mediated via cell membrane conformational changes, with changes in membrane fluidity the proposed local mode of action for RCH (e.g.…”

Section: Rapid Cold-hardening and Heat Shockmentioning

confidence: 99%

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Short-term hardening effects on survival of acute and chronic cold exposure by Drosophila melanogaster larvae

Rajamohan

Sinclair

2008

Journal of Insect Physiology

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We quantified the variation and plasticity in cold tolerance among four larval stages of four laboratory strains of Drosophila melanogaster in response to both acute (<2 hours of cold exposure) and chronic (∼7 hours of cold exposure) cold exposure. We observed significant differences in basal cold tolerance between the strains and among larval stages. Early larval instars were generally more tolerant of acute cold exposures than 3 rd instar larvae. However, wandering larvae were more tolerant of chronic cold exposures than the other stages. Early stages also displayed a more pronounced rapid cold-hardening response than the later stages. Heat pre-treatment did not confer a significant increase in cold tolerance to any of the strains at any stage, pointing to different mechanisms being involved in resolving heat-and cold-elicited damage. However, when heat pre-treatment was combined with rapid cold-hardening as sequential pre-treatments, both positive (heat first) and negative (heat second) effects on cold tolerance were observed. We discuss possible mechanisms underlying coldhardening and the effects of acute and chronic cold exposures.

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Section: Discussionsupporting

confidence: 85%

Section: Rapid Cold-hardening and Heat Shockmentioning

confidence: 99%

Short-term hardening effects on survival of acute and chronic cold exposure by Drosophila melanogaster larvae

Rajamohan

Sinclair

2008

Journal of Insect Physiology

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“…Such phenomenon is called acclimation and is observed as an improved survival rate in a cold temperature (say ~0°C) after experiencing a mildly lower temperature than the usual living temperature (Salt, 1961). Rako and Hoffmann (2006) classified this type of plastic response into three categories: "rearing acclimation" as a continuous response to a lower temperature than the optimal living temperature during the course of development (e.g., Ohtsu et al, 1999), "cold acclimation" as a response to a moderately low temperature for a few days to several weeks (e.g., Goto, 2000;Bubliy et al, 2002) and "rapid cold hardening," as a rapid response to a sub-lethal temperature for a very short time from a few minutes to hours (e.g., Czajka and Lee, 1990). Since the seasonal temperature fluctuation is relatively mild in the temperate regions, the cold acclimation is likely to be an effective strategy for insects like Drosophila to adapt to temperature changes in their habitats.…”

Section: Introductionsupporting

confidence: 92%

“…After each period of 1°C exposure, the air temperature was programed to become 20°C in approximately 25 minutes. Then, we counted the number of survivors after 48 hours, following Rako and Hoffmann (2006). If a fly stood on its legs and walk, we identified the fly as a survivor.…”

Section: Measurement Of Cold Tolerancementioning

confidence: 99%

Cold tolerance and metabolic rate increased by cold acclimation in <i>Drosophila albomicans</i> from natural populations

2013

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Cold acclimation is one of the important factors in temperature adaptation for insects needing to make rapid adjustment to the seasonal temperature changes in their living environment. In a fruit fly species, Drosophila albomicans, which has a tropical origin and currently has a wide geographic distribution extended into Asian temperate regions, cold tolerance in terms of survival time at 1°C of adult flies reared at 25°C was substantially improved by a cold acclimation at 20°C for several days. Examining 29 isofemale lines from widely distributed natural populations, we observed a substantial variation in their acclimation response. However, the acclimation response was not necessarily stronger in the strains from the recently colonized temperate regions. A significantly stronger acclimation response was detected in male flies of the temperate strains when compared to those of the tropical strains. D. albomicans also showed stronger cold tolerance compared to its closely related species belonging to the D. nasuta subgroup. Among these strains, we detected a strong positive correlation between the cold tolerance change and the metabolic rate change upon the cold acclimation, suggesting their strong physiological association regulated by common genetic factors, which may have been the target of natural selection for the temperature adaptation. The response to deacclimation and reacclimation suggested that a systematic change in gene expressions is the main molecular mechanism for the cold acclimation to have effects on the cold tolerance and metabolic rate changes.

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“…As previously noted in D. melanogaster, the increased cold stress period was associated with accumulation of chilling injuries and the time needed for recovery increased (Rako and Hoffmann, 2006). Therefore, we collected the flies from their natural habitat and allowed them to become accustomed to the laboratory conditions, so that they could recover.…”

Section: Resultsmentioning

confidence: 99%

Downregulation of hsp22 gene expression in Drosophila melanogaster from sites located near chemical plants

Magdalena

Coipan²,

Vladimirescu³

et al. 2012

Genet. Mol. Res.

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ABSTRACT.A common physiological response of organisms to environmental conditions is variation in gene expression, especially true for genes encoding for heat shock proteins. In insects, this process has been examined for induced heat or cold stress. The putative longterm imprinted/acquired heat shock protein response due to unfriendly environmental conditions has been far less studied. The Drosophila melanogaster hsp22 gene, which has been extensively reviewed as being sensitive to different changing life conditions, was examined by qRT-PCR, using carboxy-X-rhodamine. In the present study, we focused on the detection of hsp22 level of transcription in three D. melanogaster isolates, collected from sites located near different chemical plants in Romania and subjected to one-year adaptation to laboratory conditions. In all isolates, the hsp22 gene expression was determined using the housekeeping genes Gapdh1 and UbcD10 as internal controls. According to our experimental results, the D. melanogaster hsp22 gene was significantly downregulated compared to the same gene in w 1118 iso, used as a calibrator. We showed that hsp22 could play an important role in relation to stress resistance and adaptation. This study highlights the importance of in vivo studies to demonstrate genome plasticity to overcome different damages induced by any presumed source of stress.

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Complexity of the cold acclimation response in Drosophila melanogaster

Cited by 147 publications

References 31 publications

Short-term hardening effects on survival of acute and chronic cold exposure by Drosophila melanogaster larvae

Short-term hardening effects on survival of acute and chronic cold exposure by Drosophila melanogaster larvae

Cold tolerance and metabolic rate increased by cold acclimation in <i>Drosophila albomicans</i> from natural populations

Downregulation of hsp22 gene expression in Drosophila melanogaster from sites located near chemical plants

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