Construction of higher-order cellular microstructures by a self-wrapping co-culture strategy using a redox-responsive hydrogel

Ramadhan, Wahyu; Kagawa, Genki; Moriyama, Kosei; Wakabayashi, Rie; Minamihata, Kosuke; Goto, Masahiro; Kamiya, Noriho

doi:10.1038/s41598-020-63362-4

Cited by 12 publications

(11 citation statements)

References 75 publications

(99 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[71][72][73] Cell sheets co-cultured with endothelial cells are highly vascularized to offer sufficient oxygen and nutrients and show high transplantation efficiency. 74,75 The results indicate the critical role of vascularization in successful transplantation of highly functional multilayered and 3D cell sheets.…”

Section: Vascularized Cell Sheetmentioning

confidence: 88%

Tailoring cell sheets for biomedical applications

Chen,

Nie,

Gan

et al. 2024

Smart Medicine

View full text Add to dashboard Cite

Cell sheet technology has emerged as a novel scaffold‐free approach for cell‐based therapies in regenerative medicine. Techniques for harvesting cell sheets are essential to preserve the integrity of living cell sheets. This review provides an overview of fundamental technologies to fabricate cell sheets and recent advances in cell sheet‐based tissue engineering. In addition to the commonly used temperature‐responsive systems, we introduce alternative approaches, such as ROS‐induced, magnetic‐controlled, and light‐induced cell sheet technologies. Moreover, we discuss the modification of the cell sheet to improve its function, including stacking, genetic modification, and vascularization. With the significant advances in cell sheet technology, cell sheets have been widely applied in various tissues and organs, including but not limited to the lung, cornea, cartilage, periodontium, heart, and liver. This review further describes both the preclinical and clinical applications of cell sheets. We believe that the progress in cell sheet technology would further propel its biomedical applications.

show abstract

Section: Vascularized Cell Sheetmentioning

confidence: 88%

Tailoring cell sheets for biomedical applications

Chen,

Nie,

Gan

et al. 2024

Smart Medicine

View full text Add to dashboard Cite

show abstract

Section: Engineering the Tme Using Protein‐based Hydrogelsmentioning

confidence: 99%

“…Moreover, these MTs improved the cellular function of hepatocytes when compared with cellular aggregates without the cell layer coating it. [ 215 ]…”

Section: Engineering the Tme Using Protein‐based Hydrogelsmentioning

confidence: 99%

Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models

et al. 2021

View full text Add to dashboard Cite

The establishment of tumor microenvironment using biomimetic in vitro models that recapitulate key tumor hallmarks including the tumor supporting extracellular matrix (ECM) is in high demand for accelerating the discovery and preclinical validation of more effective anticancer therapeutics. To date, ECM‐mimetic hydrogels have been widely explored for 3D in vitro disease modeling owing to their bioactive properties that can be further adapted to the biochemical and biophysical properties of native tumors. Gathering on this momentum, herein the current landscape of intrinsically bioactive protein and peptide hydrogels that have been employed for 3D tumor modeling are discussed. Initially, the importance of recreating such microenvironment and the main considerations for generating ECM‐mimetic 3D hydrogel in vitro tumor models are showcased. A comprehensive discussion focusing protein, peptide, or hybrid ECM‐mimetic platforms employed for modeling cancer cells/stroma cross‐talk and for the preclinical evaluation of candidate anticancer therapies is also provided. Further development of tumor‐tunable, proteinaceous or peptide 3D microtesting platforms with microenvironment‐specific biophysical and biomolecular cues will contribute to better mimic the in vivo scenario, and improve the predictability of preclinical screening of generalized or personalized therapeutics.

show abstract

“…However, there still exist some problems associated with the incomplete adherence of the spheroid on the substrate and difficulties in regulating the cellular movement and cell density on the outer membrane of the encapsulated cells [89]. Ramadhan et al, (2020) suggested a redox-responsive hydrogel that enables self-wrapping co-culture [39]. Its mechanism involves decomposition of hydrogels under mild reductive microenvironments, and the peeling-off of a monolayer of cells cultured on a redox-responsive hydrogel surface that self-folds to wrap other cell lines.…”

Section: Hydrogel-based Platformsmentioning

confidence: 99%

Recent Advances in Multicellular Tumor Spheroid Generation for Drug Screening

Lee

Kim

2021

Biosensors

View full text Add to dashboard Cite

Multicellular tumor spheroids (MCTs) have been employed in biomedical fields owing to their advantage in designing a three-dimensional (3D) solid tumor model. For controlling multicellular cancer spheroids, mimicking the tumor extracellular matrix (ECM) microenvironment is important to understand cell–cell and cell–matrix interactions. In drug cytotoxicity assessments, MCTs provide better mimicry of conventional solid tumors that can precisely represent anticancer drug candidates’ effects. To generate incubate multicellular spheroids, researchers have developed several 3D multicellular spheroid culture technologies to establish a research background and a platform using tumor modelingvia advanced materials science, and biosensing techniques for drug-screening. In application, drug screening was performed in both invasive and non-invasive manners, according to their impact on the spheroids. Here, we review the trend of 3D spheroid culture technology and culture platforms, and their combination with various biosensing techniques for drug screening in the biomedical field.

show abstract

Construction of higher-order cellular microstructures by a self-wrapping co-culture strategy using a redox-responsive hydrogel

Cited by 12 publications

References 75 publications

Tailoring cell sheets for biomedical applications

Tailoring cell sheets for biomedical applications

Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models

Recent Advances in Multicellular Tumor Spheroid Generation for Drug Screening

Contact Info

Product

Resources

About