Continuous production of gibberellic acid in a fixed-bed reactor by immobilized mycelia of Gibberella fujikuroi in calcium alginate beads

Saucedo, J. E. Nava; Barbotin, Jean‐Noël; Thomas, Daniel

doi:10.1007/bf00256209

Cited by 18 publications

(12 citation statements)

References 27 publications

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Section: Fungimentioning

confidence: 96%

“…Itaconic, citric and lactic acid have been produced by immobilised fungal cells (Horitsu et al, 1988). Rainina et al, 1994Vorobevaetal., 1977O'Brien et al, 1990Kinoshitaetal., 1975Keller and Lingens, 1984Khareetal., 1994Vaijaetal., 1982Eikmeieretal., 1984;Horitsu et al, 1984;Honeckeretal., 1989Eikmeieretal., 1984Vieth and Venkatasubramanian, 1979Horitsu et al, 1985Stottmeister, 1980Berger and Langhammer, 1980Kautolaetal., 1991Rymowicz, 1993Nava Saucedo et al, 1989aNava Saucedo et al, 1989bRao and Panda, 1994Moresietal., 1995Kolot, 1981Tramper et al, 1983Nadkarni and D'Souza, 1981Kautolaetal., 1985Horitsu et al, 1983 …”

Section: Production Of Amino Acidsmentioning

confidence: 99%

“…gibberellins, carotens and bikaverins) for a given type of culture (Nava Saucedo et al, 1989b;. Changes in the growth development, morphological appearance, mycelial viability under starvation conditions and induction of resting forms in postimmobilisation cultures using alginate as support under different conditions, have been observed (Nava Saucedo et al, 1989b). Significant ultrastructural differences between free and Ca-alginate immobilised mycelia have been found: the presence of large glyoxisome-like bodies and active vesicle-generating systems, the formation of endocells resulting in hyphae with up to three cell walls and the concomitant accumulation of secondary metabolites (mainly pigments) in peripheral cell compartments, the progressive development of autophagic vacuoles involved in the turnover of cell constituents (Nava Saucedo et al, 1989c).…”

Section: Fungimentioning

confidence: 99%

See 2 more Smart Citations

Gel Entrapment and Micro-Encapsulation: Methods, Applications and Engineering Principles

Willaert¹,

Baron²

1996

Reviews in Chemical Engineering

113

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Section: Fungimentioning

confidence: 96%

Section: Production Of Amino Acidsmentioning

confidence: 99%

Section: Fungimentioning

confidence: 99%

See 1 more Smart Citation

Gel Entrapment and Micro-Encapsulation: Methods, Applications and Engineering Principles

Willaert¹,

Baron²

1996

Reviews in Chemical Engineering

113

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“…A scanning densitometer (DESAGA CD 60) was used to determine the amounts ABA (Saucedo et al 1989;Yürekli et al 2001). Pure ABA (Sigma, A-7383) was used as standard.…”

Section: Analysis Of Abamentioning

confidence: 99%

Changes in antioxidant activity, total phenolic and abscisic acid constituents in the aquatic plants Myriophyllum spicatum L. and Myriophyllum triphyllum Orchard exposed to cadmium

Sivaci¹,

Sivaci²,

Sökmen³

2007

Ecotoxicology

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Changes in antioxidant activity, total phenolic and abscisic acid (ABA) constituents of Myriophyllum spicatum L. and Myriophyllum triphyllum Orchard, cadmium (Cd) aqueous macrophytes, were investigated exposed to 0, 2, 4, 6, 8, 16 mg l(-1) Cd concentrations. M. triphyllum exhibited strong antioxidant activity but not M. spicatum before and after exposure. Free radical scavenging activity of M. triphyllum was significantly affected from the Cd concentrations and a significant increase was observed at 6 mgl(-1) Cd concentration. Total phenolic constituent and ABA concentration of M. triphyllum is higher than that of M. spicatum with or without heavy metal exposure (P < 0.05). While total phenolic constituents of both species were not significantly affected from Cd concentrations except for 6 mgl(-1) Cd concentration ABA contents did. ABA content of M. triphyllum increased from 1.81 +/- 0.10 microg g(-1 )(control) to 5.13 +/- 0.15 microg g(-1) at 16 mg l(-1) Cd concentration and increase was from 0.59 +/- 0.08 microg g(-1) (control) to 2.05 +/- 0.10 microg g(-1) for M. spicatum at the same Cd concentration. Both species accumulated ABA indicating submerge plants can also accumulate ABA and its concentration increase with increasing Cd concentration. Such studies as this one may be important for evaluation of the metabolic variations of toxic metal tolerant macrophytes that grown in polluted aqueous ecosystem.

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“…Gibberellic acid is the most important and its effects on higher plants are: marked stem elongation, reversal of dwarfism, promotion of fruit setting, breaking of dormancy, acceleration of seed fermentation, among others (Brü ckner and Blechschmidt 1991;Tudzynski 1999). Currently, gibberellic acid is microbiologically produced in a submerged culture (SmF) fashion but another fermentation techniques such as solid sate fermentation or with immobilized mycelium are also reported (Heinrich and Rehm 1981;Jones and Pharis 1987;Lonsane 1987, 1988;Nava Saucedo et al 1989;Escamilla et al 2000;Gelmi et al 2000Gelmi et al , 2002. Nevertheless stirred tank bioreactors with or without a fed-batch scheme have been the most employed in gibberellic acid production.…”

Section: Introductionmentioning

confidence: 96%

Hydrodynamics, mass transfer and rheological studies of gibberellic acid production in an airlift bioreactor

Chávez-Parga

González‐Ortega

Negrete-Rodríguez

et al. 2006

World J Microbiol Biotechnol

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Although a lot of research has been done into modelling microbial processes, the applicability of these concepts to problems specific for bioreactor design and optimization of process conditions is limited. This is partly due to the tendency to separate the two essential factors of bioreactor modelling, i.e. physical transport processes and microbial kinetics. The deficiencies of these models become especially evident in industrial production processes where O 2 supply is likely to become the limiting factor, e.g. production of gibberellic acid and other organic acids. Hydrodynamics, mass transfer and rheology of gibberellic acid production by Gibberella fujikuroi in an airlift bioreactor is presented in this work. Important hydrodynamic parameters such as gas holdup, liquid velocity in the riser and in the downcomer, and mixing time were determined and correlated with superficial gas velocity in the riser. Mass transfer was studied evaluating the volumetric mass transfer coefficient, which was determined as a function of superficial gas velocity in the riser and as a function of fermentation time. Culture medium rheology was studied through fermentation time and allowed to explain the volumetric mass transfer coefficient behaviour. Rheological behaviour was explained in terms of changes in the morphology of the fungus. Finally, rheological studies let us obtain correlations for gas holdup and volumetric mass transfer coefficient estimation using the superficial gas velocity in the riser and the culture medium apparent viscosity.

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Continuous production of gibberellic acid in a fixed-bed reactor by immobilized mycelia of Gibberella fujikuroi in calcium alginate beads

Cited by 18 publications

References 27 publications

Gel Entrapment and Micro-Encapsulation: Methods, Applications and Engineering Principles

Gel Entrapment and Micro-Encapsulation: Methods, Applications and Engineering Principles

Changes in antioxidant activity, total phenolic and abscisic acid constituents in the aquatic plants Myriophyllum spicatum L. and Myriophyllum triphyllum Orchard exposed to cadmium

Hydrodynamics, mass transfer and rheological studies of gibberellic acid production in an airlift bioreactor

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