Contribution of regulatory T�cells to immune tolerance and association of microRNA‑210 and Foxp3 in preeclampsia

Abstract

Increasing evidence suggests that an exaggerated maternal systemic inflammatofrery response may play a central role in the pathogenesis of preeclampsia (PE). Considering the growing evidence on microRNAs (miRNAs) and tissue-specific regulators of gene expression, we investigated the potential association of miR-210 and forkhead box p3 (Foxp3) in preeclamptic patients. Serum levels of the cytokines interleukin (IL)-6, IL-10, IL-17, and transforming grown factor-β1 were detected with ELISA. Reverse-transcription-quantitative polymerase chain reaction was performed to detect mRNA expression for maternal placenta retinoic acid-related orphan receptor C, Foxp3 and miRNA (miR)-210. Foxp3 protein expression was evaluated by western blot analysis. Serum levels of cytokines IL-10 were significantly lower in preeclamptic patients than in normal pregnant women. mRNA expression of Foxp3 was significantly lower in placenta of PE. mRNA expression of miR-210 was significantly increased in PE. Results of western blot analysis indicated that Foxp3 protein expression was lower in PE than in normal pregnant women. Our data suggest that PE manifests as a decreased number of regulatory T cells (Tregs), which regulate maternal tolerance of the fetus. In placenta from women with PE, compared with normal pregnant women, mRNA expression of Foxp3 was significantly decreased, and expression of miR-210 was significantly increased.

The Role of Epigenetics in Placental Development and the Etiology of Preeclampsia

“…It is possible that complications during implantation trigger an immune response which would create a pro-inflammatory environment, activating NFκ-B signaling, causing aberrant expression of miR-210 and all consequent downstream cascades. Recently, Chen and collaborator (2019) analysed the inflammatory profile of preeclamptic women, compared to patients which experienced healthy pregnancie [274]. The concentrations of proinflammatory cytokines (IL-6, IL-17) were higher in plasma samples from peripheral blood in the preeclampsia group.…”

Histological changes in placental rat apoptosis via FasL and cytochrome c by the nano-herbal Zanthoxylum acanthopodium

“…Based on placental histology, FasL expression was lower than cytochrome c expression. Moreover, FasL expression on the surface was lower in T cell PE than in normal pregnancies ( Chen et al, 2019 ). Decreased FasL expression was observed with the administration of EVOO ( Fig.…”

T lymphocytes and preeclampsia: The potential role of T‐cell subsets and related MicroRNAs in the pathogenesis of preeclampsia

“…Additionally, the overexpression of miRNA‐210 can suppress the expression of Foxp3 and impair Treg cell functions 106 . The expression of miR‐210, which targets Foxp3 and inhibits Treg cell functions, is high in the PE placenta 107 …”