Control of Thousand-Grain Weight by OsMADS56 in Rice

Zuo, Zhentao; Zhang, Zhenhua; Huang, Detian; Fan, Ye‐Yang; Yu, Sibin; Zhuang, Jie‐Yun; Yingguo, Zhu

doi:10.3390/ijms23010125

Cited by 17 publications

(7 citation statements)

References 49 publications

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“…Most important agronomic traits are controlled by a few major QTLs and many minor QTLs. Only a small number of genes underlying minor QTLs have been isolated in rice [ 5 , 13 , 23 , 48 , 49 ]. In our previous study, minor QTL qGS1-35.2 was fine-mapped using NIL populations derived from a cross between ZS97 and MY46.…”

Section: Discussionmentioning

confidence: 99%

“…Overexpression and knockout/knockdown of a same gene usually causes opposite effects [ 10 , 11 , 13 , 15 , 17 , 20 , 23 , 25 , 27 , 28 , 31 , 34 ]. However, we surprisingly discovered that overexpressing and knockout OsPUB3 MY46 both decreased GW.…”

Section: Discussionmentioning

confidence: 99%

“…At least 25 QTLs for grain shape and size in rice have been cloned. Fifteen of them mainly regulated grain length, including qTGW1.2b [ 5 ], GS2 / GL2 [ 6 , 7 ], OsLG3 [ 8 ], OsLG3b / qLGY3 [ 9 , 10 ], GS3.1 [ 11 ], GS3 [ 12 ], SG3 [ 13 ], GL3.1 / qGL3 [ 14 , 15 ], qTGW3 [ 16 ], qGL5 [ 17 ], TGW6 [ 18 ], GW6a [ 19 ], GL6 [ 20 ], GLW7 [ 21 ] and GL10 / OsMADS56 [ 22 , 23 ]. Seven other QTLs mainly regulated grain width, including GW2 [ 24 ], TGW2 [ 25 ], GS5 [ 26 ], GSE5 [ 27 ], GW6 [ 28 ], GW8 [ 29 ] and GW10 [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

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Control of Grain Shape and Size in Rice by Two Functional Alleles of OsPUB3 in Varied Genetic Background

Wang

Yingguo

et al. 2022

Plants

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Grain shape and size are key determinants of grain appearance quality and yield in rice. In our previous study, a grain shape QTL, qGS1-35.2, was fine-mapped using near-isogenic lines (NILs) derived from a cross between Zhenshan 97 (ZS97) and Milyang 46 (MY46). One annotated gene, OsPUB3, was found to be the most likely candidate gene. Here, knockout and overexpression experiments were performed to investigate the effects of OsPUB3 on grain shape and size. Four traits were tested, including grain length, grain width, grain weight, and the ratio of grain length to width. Knockout of OsPUB3 in NILZS97, NILMY46, and another rice cultivar carrying the OsPUB3MY46 allele all caused decreases in grain width and weight and increases in the ratio of grain length to width. Results also showed that the magnitude of the mutational effects varied depending on the target allele and the genetic background. Moreover, it was found that NILZS97 and NILMY46 carried different functional alleles of OsPUB3, causing differences in grain shape rather than grain weight. In the overexpression experiment, significant differences between transgenic-positive and transgenic-negative plants were detected in all four traits. These results indicate that OsPUB3 regulates grain shape and size through a complex mechanism and is a good target for deciphering the regulatory network of grain shape. This gene could be used to improve grain appearance quality through molecular breeding as well.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Control of Grain Shape and Size in Rice by Two Functional Alleles of OsPUB3 in Varied Genetic Background

Wang

Yingguo

et al. 2022

Plants

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“…Grain weight is an important component trait for increased yield. Zuo et al [ 6 ] validated a candidate gene, OsMADS56, encoding a MADS-box transcription factor. This was identified as the most important candidate gene in the grain weight QTL qTGW10-20.8 region in an earlier study.…”

Section: Introductionmentioning

confidence: 99%

Molecular Research in Rice

Subudhi

2023

IJMS

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“…Followed are three genes on two chromosomes, including GW2 (Song et al 2007 ), GS2 / GL2 / GLW2 (Hu et al 2015 ; Che et al 2016 ; Li et al 2016 ) and TGW2 (Ruan et al 2020 ) on chromosome 2, and GS5 (Li et al 2011 ), GW5 / GSE5 (Duan et al 2017 ; Liu et al 2017 ) and qGL5 (Qiao et al 2021 ) on chromosome 5. Then are two genes on two chromosomes, including GLW7 (Si et al 2016 ) and GL7 / GW7 (Wang et al 2015a , 2015b ) on chromosome 7, and GW10 (Zhan et al 2021 ) and GL10 / OsMADS56 (Zhan et al 2022 ; Zuo et al 2022 ) on chromosome 10. The remaining three are qTGW1.2b (Chan et al 2021 ), GW8 (Wang et al 2012 ) and GS9 (Zhao et al 2018 ) on chromosomes 1, 8 and 9, respectively.…”

Section: Introductionmentioning

confidence: 99%

Control of Grain Weight and Size in Rice (Oryza sativa L.) by OsPUB3 Encoding a U-Box E3 Ubiquitin Ligase

Wang

Zhang

Fan

et al. 2022

Rice

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Grain weight and size, mostly determined by grain length, width and thickness, are crucial traits affecting grain quality and yield in rice. A quantitative trait locus controlling grain length and width in rice, qGS1-35.2, was previously fine-mapped in a 57.7-kb region on the long arm of chromosome 1. In this study, OsPUB3, a gene encoding a U-box E3 ubiquitin ligase, was validated as the causal gene for qGS1-35.2. The effects were confirmed firstly by using CRISPR/Cas9-based mutagenesis and then through transgenic complementation of a Cas9-free knock-out (KO) mutant. Two homozygous KO lines were produced, each having a 1-bp insertion in OsPUB3 which caused frameshift mutation and premature termination. Compared with the recipient and a transgenic-negative control, both mutants showed significant decreases in grain weight and size. In transgenic complementation populations derived from four independent T0 plants, grain weight of transgenic-positive plants was significantly higher than transgenic-negative plants, coming with increased grain length and a less significant decrease in grain width. Based on data documented in RiceVarMap V2.0, eight haplotypes were classified according to six single-nucleotide polymorphisms (SNPs) found in the OsPUB3 coding region of 4695 rice accessions. Significant differences on grain size traits were detected between the three major haplotypes, Hap1, Hap2 and Hap3 that jointly occupy 98.6% of the accessions. Hap3 having the largest grain weight and grain length but intermediate grain width exhibits a potential for simultaneously improving grain yield and quality. In another set of 257 indica rice cultivars tested in our study, Hap1 and Hap2 remained to be the two largest groups. Their differences on grain weight and size were significant in the background of non-functional gse5, but non-significant in the background of functional GSE5, indicating a genetic interaction between OsPUB3 and GSE5. Cloning of OsPUB3 provides a new gene resource for investigating the regulation of grain weight and size.

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Control of Thousand-Grain Weight by OsMADS56 in Rice

Cited by 17 publications

References 49 publications

Control of Grain Shape and Size in Rice by Two Functional Alleles of OsPUB3 in Varied Genetic Background

Control of Grain Shape and Size in Rice by Two Functional Alleles of OsPUB3 in Varied Genetic Background

Molecular Research in Rice

Control of Grain Weight and Size in Rice (Oryza sativa L.) by OsPUB3 Encoding a U-Box E3 Ubiquitin Ligase

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