Correlates of Photoreceptor Rescue by Transplantation of Human Fetal RPE in the RCS Rat

Little, Caroline; Cox, Christopher; Wyatt, Jeffrey D.; Cerro, Coca del; Cerro, Manuel del

doi:10.1006/exnr.1997.6642

Cited by 46 publications

(28 citation statements)

References 23 publications

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“…This again poses problems when the goal is to compare visual function between the two eyes of a given animal. This is especially important, given that numerous animal studies evaluating therapeutic interventions for retinal disease, use only one eye as the treatment eye, and maintain the fellow eye as a control Kwan et al, 1999;Lawrence et al, 2000;Little et al, 1998;Lund et al, 2001b;Sagdullaev et al, 2003;Whiteley et al, 2001;Woch et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

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Optokinetic test to evaluate visual acuity of each eye independently

Thomas

Seiler

Sadda

et al. 2004

Journal of Neuroscience Methods

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A previously described optokinetic testing apparatus [Nat. Neurosci. 5 (2002) 53] was modified to measure vision in each eye separately for evaluation of monocular treatments. This apparatus consists also of a striped rotating drum. Ca. 170• of the drum are illuminated from outside and ca. 190• of the drum move behind a stationary black wall. The rat sits unrestrained in the drum center in a tube so that one eye is unexposed to the rotating stripes. Normal pigmented and retinal degenerate transgenic S334ter-3 rats were tested with the original and the modified apparatus. The usefulness of this method was tested in retinal degenerate rats with retinal transplants in one eye. In retinal degenerate animals, the amount of time (seconds) spent for head-tracking tended to be higher with the original method, possibly due to simultaneous stimulation of both eyes. In rats with retinal transplants, visual responses were significantly preserved in transplanted eyes at late stages of retinal degeneration. In conclusion, contributions from the fellow eye to the optokinetic tracking response can be limited by this testing modification, which is useful for evaluation of treatment effects to one eye.

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Section: Discussionmentioning

confidence: 99%

“…2000), as well as more complex light discrimination and maze tests Kwan et al, 1999;Little et al, 1998;Prusky et al, 2000). Another particularly effective test of visual performance measures an animal's ability to track moving stimuli.…”

Section: Introductionmentioning

confidence: 99%

Optokinetic test to evaluate visual acuity of each eye independently

Thomas

Seiler

Sadda

et al. 2004

Journal of Neuroscience Methods

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“…Furthermore, the impracticality of performing full safety testing on primary cultures from every donor further limits their wide-scale clinical use. A second is that little attention has been given to functional parameters in the systematic assessment of graft efficacy (13). Neither levels of photoreceptor rescue (4,(6)(7)(8) nor electroretinogram measurements (5) are indicators of how much vision is restored after intervention.…”

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confidence: 99%

Subretinal transplantation of genetically modified human cell lines attenuates loss of visual function in dystrophic rats

Lund

Adamson

Sauvé

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

179

118

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“…Various dissociated cells and tissue microaggregates were transplanted via subretinal and intravitreal injection and showed disorganized or incorrectly localized grafts [26][27][28][29]. Studies of retinal pigment epithelial (RPE) cells showed the importance of cellular continuity and polarity in the success of transplantation [30][31][32]. Following this, biodegradable polymer scaffolds were used to obtain organized sheets of RPE with distinct apical/basal characteristics [33].…”

Section: Polymer Scaffoldsmentioning

confidence: 99%

Synthetic Polymer Scaffolds for Stem Cell Transplantation in Retinal Tissue Engineering

2011

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Age-related macular degeneration and retinitis pigmentosa are two leading causes of irreversible blindness characterized by photoreceptor loss. Cell transplantation may be one of the most promising approaches of retinal repair. However, several problems hinder the success of retinal regeneration, including cell delivery and survival, limited cell integration and incomplete cell differentiation. Recent studies show that polymer scaffolds can address these three problems. This article reviews the current literature on synthetic polymer scaffolds used for stem cell transplantation, especially retinal progenitor cells. The advantages and disadvantages of different polymer scaffolds, the role of different surface modifications on cell attachment and differentiation, and controlled drug delivery are discussed. The development of material and surface modification techniques is vital in making cell transplantation a clinical success.

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Correlates of Photoreceptor Rescue by Transplantation of Human Fetal RPE in the RCS Rat

Cited by 46 publications

References 23 publications

Optokinetic test to evaluate visual acuity of each eye independently

Optokinetic test to evaluate visual acuity of each eye independently

Subretinal transplantation of genetically modified human cell lines attenuates loss of visual function in dystrophic rats

Synthetic Polymer Scaffolds for Stem Cell Transplantation in Retinal Tissue Engineering

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