Correlation of serum endothelin-1 with relapses of surgical treatment of diabetic maculopathy in type 2 diabetes mellitus

Mogilevskyy, S.; Panchenko, Yu.O.; Зябліцев, С. В.

doi:10.30978/cees-2019-3-34

Cited by 3 publications

(1 citation statement)

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“…Відомо, що надекспресія ET-1 обґрунтовує посилені ефекти, які визначають основні ушкодження судин при ЦД. Величезна кількість джерел присвячена пошуку асоціацій поліморфізмів генів з розвитком ЦД та ДР [11,12]. В тому числі доведена роль поліморфізмів генів ендотелінових рецепторів, які мають зв'язок з розвитком ЦД2: для rs6842241 гена EDNRA підвищення ризику було асоційовано з мінорною алеллю А; для rs5351 гена EDNRВ -з предковою алеллю С. Наявність цих алелей сприяло достеменно більш високому вмісту у крові ЕТ1 та більший вираженості ендотеліальної дисфункції [13].…”

Section: матеріали та методиunclassified

The Content of Endothelin-1 in the Blood Plasma of Patients With Diabetic Retinopathy on the Background of Type 2 Diabetes Depending on the Polymorphic Variants of the Mthfr, MTRR and MTR Genes

Rykov,

Prokopenko

2023

Med. Sci. of Ukr.

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Backround. The vascular and extravascular microcirculation of the eye is a rich source of endothelin-1 (ET-1), which can contribute to abnormal retinal hemodynamics in diabetic retinopathy. In patients with type 2 diabetes mellitus (T2DM), an increase in the level of circulating ET-1 was found, and a positive correlation between its levels in the blood was found and degree of microangiopathy. Strengthens the development of endothelial dysfunction and microvascular complications, a high level of homocysteine, which occurs due to a genetically determined deficiency of enzymes of the folate cycle, determines in the body what, because homocysteine causes a violation of the structure of endothelial cells. Aim: to study the ET-1 content in the blood plasma of patients with diabetic retinopathy against the background of type 2 diabetes, depending on the polymorphic variants of the MTHFR, MTRR and MTR genes, as an important pathogenetic pathway for the development of endothelial dysfunction. Materials and methods. The study included 83 patients (83 eyes) with T2DM, in whom non-proliferative and proliferative DR were found according to the results of an ophthalmological examination using the ETDRS scale. The control group (CG) included 35 people without diabetes, who were matched with patients by gender, age, and body mass index. Gene polymorphism was determined using real-time PCR on the automatic amplifier Gene Amp® PCR System 7500, the content of ET-1 was determined in blood plasma by the ELISA method. Conclusion. The SS genotype of the rs1801133 gene, the GG genotype of the rs1805087 gene, the AS polymorphism, and the SS genotype of the rs1801131 gene can be considered potential risk factors for the development of DR on the background of type 2 diabetes. The SS genotype of the rs1801133 gene was accompanied by a maximum 14-fold increase in ET-1 in patients with DR. The minor GG genotype of the rs1805087 gene was found only in patients with DR, and was characterized by the maximum content of ET-1. In the carriers of AS polymorphism of the rs1801131 gene, an 8-fold increase in ET-1 was found during the development of DR. The minor GG genotype of the rs1805087 gene was found only in patients with DR, and was characterized by the maximum content of ET-1. In the carriers of AS polymorphism of the rs1801131 gene, an 8-fold increase in ET-1 was found during the development of DR. The minor SS genotype of this gene was twice as common in patients, and the ET-1 content increased 5 times with the development of DR. The presence of ST polymorphism of the rs1801133 gene and the AA genotype of rs1801131 are probably factors that prevent the development of DR. The ST gene rs1801133 polymorphism was accompanied by the lowest ET-1 content. The AA genotype of the rs1801131 gene was 1.3 times less frequent, the ET-1 content in these individuals was the lowest and practically did not change during the development of DR.

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Section: матеріали та методиunclassified

The Content of Endothelin-1 in the Blood Plasma of Patients With Diabetic Retinopathy on the Background of Type 2 Diabetes Depending on the Polymorphic Variants of the Mthfr, MTRR and MTR Genes

Rykov,

Prokopenko

2023

Med. Sci. of Ukr.

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State of endothelial function, lipid spectrum and features of coronary vessels structure of rats with obesity and insulin resistance under iodine deficiency conditions

Todoriv¹,

Bagriy²,

Voronych-Semchenko³

2021

Fiziol Zh

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The aim of the study was to investigate the changes of endothelin-1 content, blood lipid spectrum parameters, structural features of coronary vessels of rats with insulin resistance and obesity under conditions of adequate iodine supply and iodine deficiency. For the modeling of insulin resistance, rats were kept on high-fructose, obesity – high-calorie, iodine deficiency – iodine deficiency diets. It was found that the development of insulin resistance, obesity and iodine deficiency was accompanied by an increase of endothelin-1 level in 2.41, 2.31 times and at 80.17% in blood serum, relative to the data in intact animals. Insulin resistance and obesity under conditions of limited iodine supply leads to the significant changes in endothelial dysfunction (increase in the level of endothelin-1 in 3.02 and 2.50 times relative to control and at 67.38 and 39.40% – relative to mono iodine deficiency) and dyslipidemia (increase in the atherogenic factor at 48.08% – 4.20 times relative to isolated insulin resistance, obesity and iodine deficiency). Such changes were consistent with the structural violations. In insulin-resistant animals focal unevenness of the outer and inner contours, their uneven thickness, areas of homogeneous enlightenment were observed under the conditions of iodine deficiency in the arterioles and minor arteries of the myocardium. In obese animals under the conditions of iodine deficiency, the vessels of the microcirculatory bed were dilated and overflown with erythrocytes. Endotheliocytes with nuclei elongated along the wall, in some places there is a swelling of the cytoplasm of endotheliocytes. In the wall of minor arteries there are transparent vacuoles, areas of homogeneous eosinophilia, which are caused by the accumulation of glycoproteins. Thus, the development of insulin resistance and obesity in iodine deficiency is accompanied by more significant changes in endothelial function and an increase in proatherogenic fractions in the blood lipid spectrum, as evidenced by changes in the structural organization of myocardial vessels than with proper iodine supply.

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Early Persistent Relapses of Diabetic Maculpathy After Surgical Treatment and Endotelin-1 Level in Patients With Type 2 Diabetes Mellitus

Panchenko

2020

VPBM

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Correlation of serum endothelin-1 with relapses of surgical treatment of diabetic maculopathy in type 2 diabetes mellitus

Cited by 3 publications

References 0 publications

The Content of Endothelin-1 in the Blood Plasma of Patients With Diabetic Retinopathy on the Background of Type 2 Diabetes Depending on the Polymorphic Variants of the Mthfr, MTRR and MTR Genes

The Content of Endothelin-1 in the Blood Plasma of Patients With Diabetic Retinopathy on the Background of Type 2 Diabetes Depending on the Polymorphic Variants of the Mthfr, MTRR and MTR Genes

State of endothelial function, lipid spectrum and features of coronary vessels structure of rats with obesity and insulin resistance under iodine deficiency conditions

Early Persistent Relapses of Diabetic Maculpathy After Surgical Treatment and Endotelin-1 Level in Patients With Type 2 Diabetes Mellitus

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