Corticotropin-releasing factor peptide antagonists: Design, characterization and potential clinical relevance

Rivier, Jean; Rivier, Catherine

doi:10.1016/j.yfrne.2013.10.006

Cited by 31 publications

(25 citation statements)

References 121 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These doses were selected from research demonstrating that the 25 ng dose infused into the BNST significantly decreased stress-induced cocaine reinstatement in rats (Erb and Stewart, 1999). For the VTA, astressin B, a newer, and slightly longer-acting nonselective CRF R1/R2 antagonist (Rivier and River, 2014) was dissolved in sterile saline and centrally infused in doses of 0.0, 0.25, 0.5 and 1.0 μg /0.5μl). Note that both drugs have been shown to be potent antagonists at the CRF-1 and CRF-2 receptor sites (Rivier and Rivier, 2014).…”

Section: Methodsmentioning

confidence: 99%

CRF antagonism within the ventral tegmental area but not the extended amygdala attenuates the anxiogenic effects of cocaine in rats

Ettenberg

Cotten

Brito

et al. 2015

Pharmacology Biochemistry and Behavior

View full text Add to dashboard Cite

In addition to its initial rewarding effects, cocaine has been shown to produce profound negative/anxiogenic actions. Recent work on the anxiogenic effects of cocaine has examined the role of corticotropin releasing factor (CRF), with particular attention paid to the CRF cell bodies resident to the extended amygdala (i.e, the central nucleus of the amygdala [CeA] and the bed nucleus of the stria terminalis [BNST]) and the interconnections within and projections outside the region (e.g., to the ventral tegmental area [VTA]). In the current study, localized CRF receptor antagonism was produced by intra-BNST, intra-CeA or intra-VTA application of the CRF antagonists, D-Phe CRF (12-41) or astressin-B. The effect of these treatments were examined in a runway model of i.v. cocaine self-administration that has been shown to be sensitive to both the initial rewarding and delayed anxiogenic effects of the drug in the same animal on the same trial. These dual actions of cocaine are reflected in the development of an approach-avoidance conflict (“retreat behaviors”) about goal box entry that stems from the mixed associations that subjects form about the goal. CRF antagonism within the VTA, but not the CeA or BNST, significantly reduced the frequency of approach-avoidance retreat behaviors while leaving start latencies (an index of the positive incentive properties of cocaine) unaffected. These results suggest that the critical CRF receptors contributing to the anxiogenic state associated with acute cocaine administration may lie outside the extended amygdala, and likely involve CRF projections to the VTA.

show abstract

Section: Methodsmentioning

confidence: 99%

CRF antagonism within the ventral tegmental area but not the extended amygdala attenuates the anxiogenic effects of cocaine in rats

Ettenberg

Cotten

Brito

et al. 2015

Pharmacology Biochemistry and Behavior

View full text Add to dashboard Cite

show abstract

“…It also plays a key role in endocrine, behavioral (anxiety/depression), autonomic (sympathetic activation) and immune responses to stress in the brain[3,4]. …”

Section: Introductionmentioning

confidence: 99%

Corticotropin-releasing factor stimulates colonic motilityviamuscarinic receptors in the rat

Kim¹,

Kim²,

Yoon³

et al. 2017

WJG

View full text Add to dashboard Cite

AIMTo measure exogenous corticotropin-releasing factor (CRF)-induced motility of the isolated rat colon and to demonstrate the effect of pharmacologic inhibition on CRF-induced motility.METHODSThe isolated vascularly-perfused rat colon was used. Luminal pressure was monitored via microtip catheter pressure transducers in the proximal and distal colon. At first, exogenous CRF was administered in a stepwise manner and the concentration of CRF yielding maximal colonic motility was selected. After recording basal colonic motility, hexamethonium, phentolamine, propranolol, atropine and tetrodotoxin were infused into the isolated colon. Initially, only the test drug was infused; then, CRF was added. The motility index was expressed as percentage change over basal level.RESULTSAdministration of 1.4, 14.4, 144 and 288 pmol/L CRF progressively increased colonic motility in the proximal and distal colon. Infusion of atropine or tetrodotoxin reduced CRF-induced motility of both the proximal and distal colon, whereas hexamethonium, phentolamine and propranolol had no effect.CONCLUSIONCRF-induced colonic motility appears to be mediated by local cholinergic signaling via muscarinic receptors. Muscarinic receptors are potential targets for counteracting CRF-induced colonic hypermotility.

show abstract

“…In addition, and in view of the lack of thoroughly validated CRF receptor antisera for immunohistochemical studies, radioligands have provided valuable means of localizing sites of functional receptor binding in situ. Peptides that have been used in this way include analogs of ovine CRF (Perrin et al, 1986), rat Ucn 1 (Perrin et al, 1999), and PS-Svg (Chen et al, 2005) as well as the antagonists, astressin (Gulyas et al, 1995;Rivier and Rivier, 2014) and antisauvagine-30 (Ruhmann et al, 1998) ]-PS-Svg, which bind both CRF receptors with relatively high affinity, have come to be most widely used in pharmacological and functional studies (Grigoriadis et al, 1996;Primus et al, 1997;Rominger et al, 1998;Ruhmann et al, 1998;Ardati et al, 1999;Sanchez et al, 1999;Skelton et al, 2000;Bakshi et al, 2002;Lawrence et al, 2002;Maillot et al, 2003;Wigger et al, 2004;Gehlert et al, 2005;Lim et al, 2005;Waser et al, 2006;Silberstein et al, 2009). Recently, a new CRF-related peptide was isolated from a different frog species, Pachymedusa dacnicolor, and termed PD-Svg (Zhou et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Pachymedusa dacnicolor–Sauvagine Analog as a New High-Affinity Radioligand for Corticotropin-Releasing Factor Receptor Studies

Perrin

Tan

Vaughan

et al. 2015

J Pharmacol Exp Ther

Self Cite

View full text Add to dashboard Cite

The corticotropin-releasing factor (CRF) peptide family comprises the mammalian peptides CRF and the urocortins as well as frog skin sauvagine and fish urophyseal urotensin. Advances in understanding the roles of the CRF ligand family and associated receptors have often relied on radioreceptor assays using labeled CRF ligands. These assays depend on stable, high-affinity CRF analogs that can be labeled, purified, and chemically characterized. Analogs of several of the native peptides have been used in this context, most prominently including sauvagine from the frog Phyllomedusa sauvageii (PS-Svg). Because each of these affords both advantages and disadvantages, new analogs with superior properties would be welcome. We find that a sauvagine-like peptide recently isolated from a different frog species, Pachymedusa dacnicolor (PD-Svg), is a high-affinity agonist whose radioiodinated analog, [ 125 ITyr 0 -Glu 1 , Nle 17 ]-PD-Svg, exhibits improved biochemical properties over those of earlier iodinated agonists. Specifically, the PD-Svg radioligand binds both CRF receptors with comparably high affinity as its PS-Svg counterpart, but detects a greater number of sites on both type 1 and type 2 receptors. PD-Svg is also ∼10 times more potent at stimulating cAMP accumulation in cells expressing the native receptors. Autoradiographic localization using the PD-Svg radioligand shows robust specific binding to rodent brain and peripheral tissues that identifies consensus CRF receptor-expressing sites in a greater number and/or with greater sensitivity than its PS-Svg counterpart. We suggest that labeled analogs of PD-Svg may be useful tools for biochemical, structural, pharmacological, and anatomic studies of CRF receptors.

show abstract

Corticotropin-releasing factor peptide antagonists: Design, characterization and potential clinical relevance

Cited by 31 publications

References 121 publications

CRF antagonism within the ventral tegmental area but not the extended amygdala attenuates the anxiogenic effects of cocaine in rats

CRF antagonism within the ventral tegmental area but not the extended amygdala attenuates the anxiogenic effects of cocaine in rats

Corticotropin-releasing factor stimulates colonic motilityviamuscarinic receptors in the rat

Characterization of a Pachymedusa dacnicolor–Sauvagine Analog as a New High-Affinity Radioligand for Corticotropin-Releasing Factor Receptor Studies

Contact Info

Product

Resources

About