Cultivation of Chlorella vulgaris on dairy waste using vision imaging for biomass growth monitoring

Peter, Angela Paul; Chew, Kit Wayne; Koyande, Apurav Krishna; Sia, Yuk-Heng; Ting, H. Y.; Rajendran, Saravanan; Munawaroh, Heli Siti Halimatul; Show, Pau Loke

doi:10.1016/j.biortech.2021.125892

Cited by 21 publications

(9 citation statements)

References 39 publications

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“…For analysis of protein, a UV-Visible spectrophotometer was used at 595 nm. Bovine serum albumin was used to create a calibration curve with values ranging from 0 to 10 mg/mL [32]. Blank samples were prepared for all measurements with 1000 µL deionized water and 250 µL of Bradford reagent.…”

Section: Microalgae Protein Extraction From Dried Biomassmentioning

confidence: 99%

Strategy Development for Microalgae Spirulina platensis Biomass Cultivation in a Bubble Photobioreactor to Promote High Carbohydrate Content

et al. 2022

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As a counter to climate change, energy crises, and global warming, microalgal biomass has gained a lot of interest as a sustainable and environmentally favorable biofuel feedstock. Microalgal carbohydrate is considered one of the promising feedstocks for biofuel produced via the bioconversion route under a biorefinery system. However, the present culture technique, which uses a commercial medium, has poor biomass and carbohydrate productivity, creating a bottleneck for long-term microalgal-carbohydrate-based biofuel generation. This current investigation aims toward the simultaneous increase in biomass and carbohydrate accumulation of Spirulina platensis by formulating an optimal growth condition under different concentrations of nitrogen and phosphorous in flasks and a bubble photobioreactor. For this purpose, the lack of nitrogen (NaNO3) and phosphorous (K2HPO4) in the culture medium resulted in an enhanced Spirulina platensis biomass and total carbohydrate 0.93 ± 0.00 g/L and 74.44% (w/w), respectively. This research is a significant step in defining culture conditions that might be used to tune the carbohydrate content of Spirulina.

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Section: Microalgae Protein Extraction From Dried Biomassmentioning

confidence: 99%

Strategy Development for Microalgae Spirulina platensis Biomass Cultivation in a Bubble Photobioreactor to Promote High Carbohydrate Content

et al. 2022

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“…The composition and proportion of the culture medium have an important impact on microbial cell growth and product synthesis (Peter et al 2021 ). Previous reports showed that the production of aromatic compounds by nonconventional yeast fermentation was improved by adding tequila vinasses into the medium (Rodriguez-Romero et al 2020 ).…”

Section: Resultsmentioning

confidence: 99%

Production of pyruvic acid with Candida glabrata using self-fermenting spent yeast cell dry powder as a seed nitrogen source

Shan

Zeng

et al. 2022

Bioresour. Bioprocess.

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Pyruvic acid is an important organic acid and a key industrial raw material. It is widely used in the chemical, agricultural, and food fields. Candida glabrata is the preferred strain for pyruvic acid production. The waste yeast cell for pyruvic acid fermentation with C. glabrata are rich in protein, amino acid, nucleic acid, and vitamins, as potential and cost-effective nitrogen source raw material. In this study, the potential of C. glabrata to produce pyruvic acid using spent yeast cell dry powder was evaluated. When 30 g/L of spray-dried spent yeast cell powder was used as the seed nitrogen source, a high titer of pyruvic acid was obtained. The pyruvic acid production reached 63.4 g/L with a yield of 0.59 g/g in a 5 L bioreactor. After scale-up to a 50 L bioreactor using the fermented spent yeast cell dry powder as a seed nitrogen source, 65.1 g/L of pyruvic acid was harvested, with a yield of 0.61 g/g. This study proposes a promisingapproach for increasing the pyruvic acid titer and reducing the costs. Graphical Abstract

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“…A técnica já foi aplicada na quantificação de curcuminóides em cúrcuma, no qual os resultados obtidos foram muito próximos, não havendo diferenças significativas quando comparado com os resultados obtidos via espectrofotometria (Supannarach e Thanapatay, 2008). A mesma metodologia, aplicada a variação e intensidade de cor foi utilizada no monitoramento do crescimento da alga Chlorella vulgaris (Peter et al, 2021).…”

Section: Discussionunclassified

Use of RGB as a Quantitative Method Applied to the Teaching of Analytical Chemistry

VELOSO¹,

SACRAMENTO²,

ROYO³

2022

PTQ

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Background: The use of alternatives for teaching analytical chemistry in undergraduate studies has been a challenge for teachers since most methods require high-cost equipment and functional analytical skills. This work presents the use of RGB, an additive color system n digital media, as a method of quantifying of a given analyte in samples. Aim: To analyze different samples containing different concentrations of coffee, comparing them using two techniques: 1) spectrophotometry and 2) RGB, and then perform the calculations for the determination of an unknown concentration. Methods: Eight samples were prepared, six with defined concentrations of coffee, one in white, and one without a defined concentration. Method 1 used the spectrophotometer to read the absorbance at 420 nm wavelength for melanoidin reading, in method 2 the RGB was used, positioning buckets in front of a digital screen emitting light at the corresponding wavelength. Microsoft Powerpoint was used to collect RGB and Microsoft Excel data for data processing. Results: The data obtained through the analytical equipment, as well as those obtained via RGB, were able to estimate the unknown concentration of coffee, both techniques estimated a concentration between 3 and 4%, both with the equation of the line and r² capable of estimating values corresponding to the concentrations of the samples. Discussion: The RGB technique has been studied in recent decades, mainly for colorimetric analyses, since it makes the study or laboratory experiments available in an accessible way. Bringing the student closer to the spectrophotometric technique, promoting scientific development, and elaborating the hypothesis about using technology as an analysis tool. Conclusions: Experimental practices promote the development of research, which guide both teachers and students in the use of new technologies and in the development of new activities aimed at teaching, this work in question opens a range for the use of tools and methods of easy access and low cost.

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Cultivation of Chlorella vulgaris on dairy waste using vision imaging for biomass growth monitoring

Cited by 21 publications

References 39 publications

Strategy Development for Microalgae Spirulina platensis Biomass Cultivation in a Bubble Photobioreactor to Promote High Carbohydrate Content

Strategy Development for Microalgae Spirulina platensis Biomass Cultivation in a Bubble Photobioreactor to Promote High Carbohydrate Content

Production of pyruvic acid with Candida glabrata using self-fermenting spent yeast cell dry powder as a seed nitrogen source

Use of RGB as a Quantitative Method Applied to the Teaching of Analytical Chemistry

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