Cyclin A1 is required for meiosis in the male mouse

Liu, Dong; Matzuk, Martin M.; Sung, Weng Kong; Guo, Qiu; Wang, Pei; Wolgemuth, Debra J.

doi:10.1038/3855

Cited by 343 publications

(307 citation statements)

References 23 publications

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“…Many of these genes encode proteins specifically involved in the control of the meiotic cell cycle, such as cyclin A1 (Table 2) (Liu et al, 1998), cdk4-inhibitors p18 and p19 (Table 2) (Zindy et al, 2001), A-myb (Table 2) (Toscani et al, 1997), Nek2 (Table 9) (Di Agostino et al, 2002), but in many cases their expression reflects meiotic accumulation of transcripts destined to be translated later during spermiogenesis, such as testis-specific lactate dehydrogenase (Table 7) (Li et al, 1998), testis-specific poly(A) polymerase b (Table 8) Kashiwabara et al, 2002), calmegin (Table 9) (Ikawa et al, 1997), preproacrosin (Table 5) (Kremling et al, 1991), fertilin b (Table 5) (Cho et al, 1998), Trf2 (Table 3) (Martianov et al, 2001), MSJ-1 (Table 5) (Berruti and Martegani, 2001), Tpx1 (Table 5) (Kasahara et al, 1989), Tekt1 (Table 5) (Larsson et al, 2000), Tesp1 (Table 5) (Kohno et al, 1998) and so on. It is noteworthy that the spermatocyte-specific expression of a large number of genes encoding enzymes is involved in glycolysis and gluconeogenesis, beside that of Pgk2, encoding a well known meiotic isoform of phosphoglycerate kinase (Boer et al, 1987) (Table 7).…”

Section: Resultsmentioning

confidence: 99%

Analysis of the gene expression profile of mouse male meiotic germ cells

Rossi

Dolci

Sette

et al. 2004

Gene Expression Patterns

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Wide genome analysis of difference in gene expression between spermatogonial populations from 7-day-old mice and pachytene spermatocytes from 18-day-old mice was performed using Affymetrix gene chips representing , 12,500 mouse known genes or EST sequences, spanning approximately 1/3rd of the mouse genome. To delineate differences in the profile of gene expression between mitotic and meiotic stages of male germ cell differentiation, expressed genes were grouped in functional clusters. The analysis confirmed the previously described pre-meiotic or meiotic expression for several genes, in particular for those involved in the regulation of the mitotic and meiotic cell cycle, and for those whose transcripts are accumulated during the meiotic stages to be translated later in post-meiotic stages. Differential expression of several additional genes was discovered. In few cases (pro-apoptotic factors Bak, Bad and Bax), data were in conflict with the previously published stage-dependent expression of genes already known to be expressed in male germ cells. Northern blot analysis of selected genes confirmed the results obtained with the microarray chips. Six of these were novel genes specifically expressed in pachytene spermatocytes: a chromatin remodeling factor (chrac1/YCL1), a homeobox gene (hmx1), a novel G-coupled receptor for an unknown ligand (Gpr19), a glycoprotein of the intestinal epithelium (mucin 3), a novel RAS activator (Ranbp9), and the A630056B21Rik gene (predicted to encode a novel zinc finger protein). These studies will help to delineate the global patterns of gene expression characterizing male germ cell differentiation for a better understanding of regulation of spermatogenesis in mammals. q

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Section: Resultsmentioning

confidence: 99%

Analysis of the gene expression profile of mouse male meiotic germ cells

Rossi

Dolci

Sette

et al. 2004

Gene Expression Patterns

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“…Gene disruption revealed that cyclin A1 is required for the meiotic progression of male germ cells in mice (Liu et al, 1998). Recent findings also suggest that, in normal meiotic male germ cells in mice, cyclin A1 participates in the regulation of protein kinases such as cdc25s or phosphatases, critical to the G2-M transition, probably through involvement in the initial amplification of MPF by means of the activation of cdc25-phosphatase phosphorylation (Liu et al, 2000).…”

Section: Discussionmentioning

confidence: 98%

“…Studies of A-type cyclins in mammalian gametogenesis indicate that cyclin A2 is expressed from the spermatogonium stage to the preleptotene spermatocyte stage and that cyclin A1 is expressed only from the pachytene spermatocyte stage to the end of meiotic division (Sweeney et al, 1996). Furthermore, cyclin A1 is required for the entry of spermatocytes into the first meiotic division in male mice (Liu et al, 1998). Although it is known that two distinct A-type cyclins (A1 and A2) are present, information on the activities of these two cyclins during gametogenesis is scarce, and, with respect to spermatogenesis, it is generally limited to mice.…”

Section: Introductionmentioning

confidence: 99%

Cloning of cDNAs and the differential expression of A‐type cyclins and Dmc1 during spermatogenesis in the Japanese eel, a teleost fish

Kajiura-Kobayashi

Kobayashi

Nagahama

2005

Developmental Dynamics

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We cloned A-type cyclins (cyclins A1 and A2) and Dmc1 cDNAs from the eel testis. Cyclin A1 mRNA was predominantly expressed in the livers, ovaries, and testes of the eels. In contrast to cyclin A1 mRNA, a very high expression of cyclin A2 mRNA was observed in the brains, livers, kidneys, spleens, ovaries, and testes of the eels. Dmc1 mRNA was predominantly expressed in the testes and ovaries; expression in the brain was also detected. In the eel testis, a few type-A spermatogonia incorporating 5-bromo-2 -deoxyuridine (BrdU) were seen before the initiation of spermatogenesis by hormonal induction. On day 1 after hormonal induction, the number of BrdU-labeled spermatogonia increased remarkably, and after 3 and 6 days, many labeled type-B spermatogonia were also observed. The expression of cyclin A2 increased 1 day after the induction of spermatogenesis and reached a plateau after 6 days, when many type-B spermatogonia with high proliferative activity were found. In contrast, the expression of cyclin A1 mRNA was detected after 9 days, coincident with the first appearance of spermatocytes. Cyclin A1 mRNA was localized in germ cells of all stages, from primary spermatocytes to round spermatids, whereas cyclin A2 mRNA was specifically localized in spermatogonia, secondary spermatocytes, round spermatids, and testicular somatic cells, including Sertoli cells. Dmc1 was localized only in the earlier stages of primary spermatocytes; before this stage, cyclin A1 mRNA was not detectable. Overall, cyclin A2, Dmc1, and cyclin A1 are expressed in spermatogenic cells sequentially before and during meiosis in the eel testis. Developmental Dynamics 232: 1115-1123, 2005.

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“…21 We detected meiotic induction of cyclin A1 and CDK2, which are essential for spermatogenesis but not for mitosis. [9][10][11][12] CDKN2c (p18 INK4c ) and CDKN2d (p19 INK4d ), whose expression also markedly increased, conferred infertility to double-knockout mice. 19 In contrast, CDKN1b (p27 KIP1 ) expression was almost absent in our analysis (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…We created transgenic mice that expressed both b-galactosidase and EGFP under the control of the murine and the human cyclin A1 promoter, respectively. 12,14,26 Expression of the reporter genes was then analyzed in adjacent testis sections prepared from mice at postnatal days 1,4,8,11,14,18,21,26 and 34 by X-gal staining (b-galactosidase) and fluorescence microscopy (EGFP; Fig. 6a).…”

Section: Human and Murine Cyclin A1 Promoter Activity In Testis Develmentioning

confidence: 99%

Expression patterns of mitotic and meiotic cell cycle regulators in testicular cancer and development

Diederichs

Bäumer

Schultz

et al. 2005

Intl Journal of Cancer

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Mitotic and meiotic cell cycle regulation is essential for normal development and tumor prevention. The underlying molecular mechanisms are not completely characterized. The aim of our analysis was to derive a global expression map of cell cycle regulators in mitosis and meiosis. First, the expression of cyclins, CDKs and CDK inhibitors was determined during postnatal testis maturation in mice using microarrays and quantitative RT-PCR. The abundance of cyclins A1, B2, K, M4, CDK2, all CDKLs, CDKN2c, CDKN2d and INCA1 increased during testis maturation. In contrast, cyclins A2, B1, D2, G1, G2, CDK1, CDK4 and CDK2AP1 showed a maturation-associated decrease. Gene expression profiles of isolated germ cells and testicular somatic cells confirmed these results. Second, we determined cyclin expression patterns in human normal and malignant testis samples (n 5 36) modeling the reciprocal difference between meiosis and mitosis. Testicular tumors strictly expressed cell cycle regulators identified in mitotically dividing germ cells. Expression of several transcripts was histology-specific in testicular tumors, providing novel molecular markers and potential therapeutic targets. Taken together, our data provide a comprehensive expression map of cell cycle regulators at the switch between mitosis and meiosis in testis development and in cancerogenesis. ' 2005 Wiley-Liss, Inc.Key words: cyclin; testicular tumorigenesis; meiosis; mitosis Multicellular organisms rely on a strict regulation of cell division and proliferation. Dysregulation of the mitotic cell cycle has been recognized as a hallmark of the development of malignant diseases. 1 Therefore, a deeper insight into the molecular mechanisms regulating mitosis contributes to the understanding of the tumorigenic process.The molecular machinery functioning in meiotic and postmeiotic germ cells is highly divergent from that used by germ cells in the mitotic cell cycle. 2 The germ line is unique compared to other cell lineages since strict regulation of both, the mitotic and the meiotic cell cycle, is indispensable for the generation of gametes. The seminiferous tubules in the testes of newborn mice only contain undifferentiated spermatogonia and immature Sertoli cells, which both exert mitotic proliferation. 3 The postnatal development is divided in three main stages: mitotic proliferation of spermatogonial stem cells; meiotic differentiation from primary spermatids to haploid early round spermatids; and spermiogenesis in which spermatids are reorganized to mature spermatozoa. The meiotic cell cycle starts in mice around postnatal day (P) 11, with the first appearance of haploid spermatids between P17 and P21. 4 With increasing age, the undifferentiated spermatogonia develop into differentiating spermatogonia of A and B types and subsequently undergo meiosis and spermiogenesis until fertility is reached at 35-37 days of age.The molecular mechanisms governing the stages of mitotic and meiotic cell cycles remain incompletely understood. Several groups have analyzed the impact...

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Cyclin A1 is required for meiosis in the male mouse

Cited by 343 publications

References 23 publications

Analysis of the gene expression profile of mouse male meiotic germ cells

Analysis of the gene expression profile of mouse male meiotic germ cells

Cloning of cDNAs and the differential expression of A‐type cyclins and Dmc1 during spermatogenesis in the Japanese eel, a teleost fish

Expression patterns of mitotic and meiotic cell cycle regulators in testicular cancer and development

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