Cytogenetic Biomonitoring in Buccal Mucosal Cells from Municipal Solid Waste Collectors

Abstract

Waste collectors collect, transport, and process the garbage produced by people living in the city. Nowadays, this activity requires special attention due to the environmental impact of garbage and its potential consequences on human health. The aim of this study was to evaluate potential cytotoxic and mutagenic effects of garbage collection on waste collectors. For this purpose, a total of 47 male waste collectors aged from 24 to 53 years were included in the experimental group. A total of 30 men matched by age were used as the control group. Cytotoxicity and mutagenicity were analyzed by micronucleus test in buccal mucosaI cells. No statistically significant difference (p>0.05) in the frequency of micronuclei was detected in the waste collectors when compared to controls. Nevertheless, higher frequencies of karyolysis and pyknosis (p<0.05) were detected in buccal mucosaI cells from waste collectors when compared to matched controls. Taken together, our results indicate that waste collectors comprise an at-risk group as a result of increased cytotoxicity apparent from buccal mucosa cells.

Genomic Instability in Peripheral Blood and Buccal Mucosal Cells of Marijuana Smokers: The Impact of Tobacco Smoke

“…Among them, micronucleus and single cell gel comet assays are simple, low cost, reproducible and non-invasive methodologies for investigating cytogenetic damage and DNA strand breaks in eukaryotic cells, respectively (Tice et al, 2000;Bonassi et al, 2011). Previous studies conducted by our research group have demonstrated that micronucleus and single cell gel comet assays are important tools for biomonitoring people Editorial Process: Submission:02/26/2019 Acceptance:11/10/2019 exposed to chemical agents and/or conditions considered to be suspect (Guilheiro et al, 2014;Souza et al, 2016;Andrade et al, 2017;Da Silva et al, 2018).…”

Genomic Instability in Buccal Mucosal Cells of Municipal Street Sweepers as Evaluated by Micronucleus Test

“…In order to monitor cytotoxic effects, the frequencies of karyorrhexis, karyolysis and pyknosis were performed in this study. In an earlier study conducted by our research group, it was demonstrated that waste collectors increased metanuclear alterations indicative of cytotoxicity (23). Such findings motivated us to investigate if, and to what extent, street sweepers possess elevated cytotoxicity in buccal mucosa cells as well.…”

Association between occupational exposure to domestic solid waste and dental caries: a cross-sectional study

“…Therefore, direct occupational contact with domestic solid waste was not associated with dental caries. However, waste collectors are exposed daily to a variety of occupational hazards, including infectious pathogens, that can enter the host through direct contact with waste or inhalation, resulting in a wide range of other health effects (Ivens et al 1998;Kuijer et al 2010;Andrade et al 2017;Brina et al 2018;Da Silva et al 2018;Zolnikov et al 2018). The exposition to virus, fungi and bacteria from the domestic waste may cause a dysbiosis of the oral microbiome, driving the polymicrobial communities towards pathogenicity with a particular emphasis on cariogenic biofilm development, which will be able to produce acid and cause the demineralization of the tooth surface.…”

“…There is increasing evidence that waste management work has been associated with respiratory disorders (Bünger et al 2007), gastrointestinal disorders, musculoskeletal disorders, dermatological problems, hearing loss, and a high prevalence of viral infections (Kuijer et al 2010;Zolnikov et al 2018). Adverse oral health effects were also identified, including tooth damage, toothache, as well as increased cytotoxicity in buccal mucosa cells (Ivens et al 1998;Andrade et al 2017;Brina et al 2018;Da Silva et al 2018;Zolnikov et al 2018). Of particular importance, strong evidence is available to show that biological agents' exposure exceeded generally accepted recommendations in waste collecting (Kuijer et al 2010).…”