Cytohistological studies on the action of vitamin D3 and stigmasterol on Phaseolus vulgaris roots growing in vitro

Effect of Vitamins on <i>In Vitro</i> Organogenesis of Plant

“…In a control treatment without any vitamins 43.75% of the roots were longer than 14 mm, while vitamin D 3 addition achieved 78.75%. The effect of vitamin D 3 shown by Boland et al [22] at 10 -9 M, on root growth was associated with an uptake of calcium ions, an increase in cell elongation in root zone at 0.5 -1 mm from the apex, and stimulation of mitotic division of meristematic cells.…”

“…Vitamin D 3 stimulates rooting of Phaseolus vulgaris L. in culture [22]. In a control treatment without any vitamins 43.75% of the roots were longer than 14 mm, while vitamin D 3 addition achieved 78.75%.…”

Presence of Estrogens and Estrogen Receptor-like Proteins in Solanum glaucophyllum

“…Various steroids derived from plant and animal sources have been shown to affect in vitro cell growth and tissue differentiation in algae and vascular plant systems (1,(5)(6)(7)(8). However, the mechanism of action of endogenously synthesized steroids in plant cells is still poorly understood.…”

Presence of vitamin D3 receptor (VDR)‐like proteins in Solanum glaucophyllum

“…The biological effects of 1α,25(OH) 2 ‐vitamin D 3 [1α,25(OH) 2 D 3 ] are mediated by a specific vitamin D receptor (VDR) protein, completely cloned several years ago (McDonell et al 1987, Baker et al 1988). Several types of bacteria, fungi, algae, and vascular plants in general, contain steroids (Wasserman et al 1976, Geuns 1978, Buchala and Schmid 1979, Grunwald 1980, Mudd 1980, Mandava et al 1982, Feldman et al 1982, Boland 1986, Weissenberg et al 1989, Agarwal 1993, Curino et al 1998, Skliar et al 2000) and have been shown to undergo physiologic changes in response to these compounds, including vitamin D 3 , 25(OH)‐vitamin D 3 [25(OH)D 3 ] and 1α,25(OH) 2 D 3 (Geuns 1978, Buchala and Schmid 1979, Vega et al 1988, Talmon et al 1989, Agarwal 1993). The presence of vitamin D 3 and its hydroxylated derivatives, in particular 1α,25(OH) 2 D 3 , has been detected in an appreciable number of flowering plants, including Solanum glaucophyllum Desf.…”

“…The biological effects of 1a,25(OH) 2vitamin D 3 [1a,25(OH) 2 D 3 ] are mediated by a specific vitamin D receptor (VDR) protein, completely cloned several years ago (McDonell et al 1987, Baker et al 1988. Several types of bacteria, fungi, algae, and vascular plants in general, contain steroids (Wasserman et al 1976, Geuns 1978, Buchala and Schmid 1979, Grunwald 1980, Mudd 1980, Mandava et al 1982, Feldman et al 1982, Boland 1986, Weissenberg et al 1989, Agarwal 1993, Curino et al 1998, Skliar et al 2000 and have been Abbreviations -1a,25(OH) 2 D 3 , 1a,25(OH) 2 -vitamin D 3 ; BSA, bovine serunm albumin; 2,4-D, 2,4-dichlorophenoxyacetic acid; DAPI, 4#,6-diamidino-2-phenylindole dihydrochloride; DTT, dithiothreitol; ECL, enhanced chemiluminescence; HRE, hormone response element; MAPK, mitogen-activated protein kinase; PBS, phosphate-buffered saline; PBS-T, phosphate-buffered saline containing 0.1% Tween-20; PCV, packed cell volume; PI, propidium iodide; PMSF, phenylmethylsulfonyl fluoride; PVDF, polyvinylidene fluoride; SDS, sodium dodecyl sulfate; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; VDR, vitamin D receptor. shown to undergo physiologic changes in response to these compounds, including vitamin D 3 , 25(OH)-vitamin D 3 [25(OH)D 3 ] and 1a,25(OH) 2 D 3 (Geuns 1978, Buchala and Schmid 1979, Talmon et al 1989, Agarwal 1993. The presence of vitamin D 3 and its hydroxylated derivatives, in particular 1a,25(OH) 2 D 3 , has been detected in an appreciable number of flowering plants, including Solanum glaucophyllum Desf.…”