Cytokine Analysis in Lesions Refractory to Endodontic Treatment

Henriques, Luiz Carlos Feitosa; Brito, Luciana Carla Neves de; Tavares, Warley Luciano Fonseca; Vieira, Leda Quércia; Sobrinho, Antônio Paulino Ribeiro

doi:10.1016/j.joen.2011.08.007

Cited by 59 publications

(59 citation statements)

References 37 publications

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“…Cleaning and shaping of the root canals were completed by using ProTaper nickel-titanium files (Dentsply Maillefer, Ballaigues, Switzerland) in conjunction with 5.2% sodium hypochlorite. The clinical procedures were performed as previously described (11). Briefly, the samples were collected immediately after root canal cleaning to characterize the mRNA expression profiles of cytokines, chemokines, and T-cell genes.…”

Section: Sample Collectionmentioning

confidence: 99%

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T-Lymphocyte and Cytokine Expression in Human Inflammatory Periapical Lesions

Brito

Teles²,

Teles³

et al. 2012

Journal of Endodontics

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Section: Sample Collectionmentioning

confidence: 99%

“…Total RNA was extracted from each sample with TRIzol reagent (GIBCO/BRL Laboratories, Grand Island, NY) as described elsewhere (11). RNA was then stored at À70 C.…”

Section: Sample Preparationmentioning

confidence: 99%

T-Lymphocyte and Cytokine Expression in Human Inflammatory Periapical Lesions

Brito

Teles²,

Teles³

et al. 2012

Journal of Endodontics

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“…3,4,10 Regarding IL-17A, all the granuloma lesions analyzed in the present study were positive for this cytokine, which is in agreement with other studies. 4,10,11,12 The percentage of labeling area was, however, lower than that found for abscesses.…”

Section: Discussionmentioning

confidence: 58%

“…8,9 Some studies have investigated the Th17 phenotype in periapical cysts and granulomas. 4,10,11,12 An association between neutrophil infiltrate and IL-17A expression has been partially detected in symptomatic periapical granulomas. 4 but IL-17A expression is not known to occur in periapical abscesses.…”

Section: Declaration Of Interestsmentioning

confidence: 99%

Analysis of Interleukin 17A in periapical abscess and granuloma lesions

2016

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Interleukin 17A (IL-17A) is a proinflammatory cytokine responsible for the initiation and propagation of inflammation. One of its actions is the recruitment of neutrophils to the site of infection. The aim of this study was to investigate whether there is association between IL-17A expression and neutrophil infiltration in periapical abscesses and periapical granulomas, as well as to find which type of T lymphocyte effector (CD4 + or CD8 + ) expresses IL-17A in these lesions. Elastase, CD4, CD8, and IL-17A were analyzed by immunohistochemistry and immunofluorescence, in the biopsies of periapical lesions. Abscess lesions exhibited the highest labeling area for IL-17A (p = 0.011). During double immunofluorescence staining, there were significantly more CD4+ cells compared to CD8 + /IL-17A + cells, both in the abscesses (p = 0.025) and granulomas (p = 0.011). In conclusion, IL-17A was intensively expressed in periapical abscesses rich in neutrophils. The high percentage of IL-17A in these cases suggests the participation of this cytokine particularly in the acute stages of the inflammatory process of the periapical lesions.

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“…In addition, high levels of TNF-α were produced in refractory lesions, as well as in cases of chronic lesions and exudate, compared to healthy teeth (Henriques et al, 2011). In this regard, the presence/persistence of microorganisms in the pulp and periapical area corresponds to the maintenance of an exacerbated immune response that leads to the start of periradicular bone resorption and its perpetuation (Siqueira et al, 2001).…”

Section: Pulpal and Periapical Infectionsmentioning

confidence: 99%