Cytological Assessment of Doubled Haploids in Summer Squash (Cucurbita Pepo L.)

Домблидес, Е. А.; Кан, Л. Ю.; Химич, Г. А.; Коротцева, И. Б.; Домблидес, А. С.

doi:10.18619/2072-9146-2018-6-3-7

Cited by 4 publications

(1 citation statement)

References 7 publications

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“…ovifera (L.) Decker относятся к семейству Cucurbitaceae, роду Cucurbita, виду Cucurbita реро L. Семейство Cucurbitaceae, по разным оценкам, состоит из 98-118 родов и 825-975 видов, в основном распространенных в тропических и субтропических регионах, что говорит о огромном видовом морфологическом разнообразии (9,10). Так, например, если для Cucumis sativus число хромосом равно 14 (2x=2n=14), для Citrullus vulgaris число хромосом равно 22 (2x=2n=22), то для Cucurbita число хромосом уже будет равно 40 (2x=2n=40) [11,12].…”

Section: Introductionunclassified

The genetics of fruit color in Cucurbita pepo L.

Korottseva,

Ermolaev,

Khimich

2023

Ovoŝi Rossii

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Species Cucurbita pepo L., which includes marrow, squash, hard-barked pumpkin and a number of other crops, has a high polymorphism both in plant structure and in shape, size, color of the bark and fruit pulp. The color of the bark of the fruits of this species can be white, cream, yellow, green, orange with or without a pattern. Appearance can greatly influence the marketing success of new hybrids among the target audience. This is explained by the fact that there are strong regional consumer preferences regarding the color of fruits, although most often, on the shelves of chain stores, in fresh or canned form, you can find fruits of bright, saturated colors and shades. At the same time, brightly colored fruits are more often used for canning, while light-colored ones are used for processing into caviar [1]. Paris H.S. and Brown R.N. summarized the identification of more than 80 gene loci that affect fruit color in Cucurbita pepo L., some of which are multi-allelic [2]. The color of the fruit in hard-barked pumpkin depends on the presence and interaction between the loci responsible for this trait, and many other factors, including growing conditions [3,4]. In cucurbits, in different phenophases, different genes are responsible for color [5–7]. All this makes the selection of summer squash and scallop on the basis of the color of the fruit bark very difficult. Knowing the genetics of coloration can make this task easier. In this article, we tried to summarize the results of studies presented in foreign literature sources on the study of Cucurbita pepo L. genes responsible for the color of the surface of the fruit and pulp, its intensity, the presence or absence of a pattern of various colors and configurations. No domestic studies on this topic could be found.

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Section: Introductionunclassified

The genetics of fruit color in Cucurbita pepo L.

Korottseva,

Ermolaev,

Khimich

2023

Ovoŝi Rossii

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Advances in Summer Squash (Cucurbita pepo L.) Molecular Breeding Strategies

Ibrahim

Salem

Abdein

et al. 2023

Smart Plant Breeding for Vegetable Crops in Post-Genomics Era

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DEVELOPMENT OF ELEMENTS OF TECHNOLOGY FOR PLANTING MATERIAL OF LETTUCE (<i>LACTUCA SATIVA L.</i>) ON VIRUS-FREE BASIS USING METHODS OF BIOTECHNOLOGY

et al. 2019

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The article presents the results of research on the production in vitro of regenerated plants from the seeds of cultivars of lettuce (Lactuca sativa L.) Emerald, Bouquet, Chameleon (FSBSI Federal Scientific Vegetable Center), susceptible of aspermia tomato (Tomato aspermy cucumovirus) – AsTV. Seeds of strongly susceptible to AsTV varieties of salad Chameleon and Bouquet were subjected to thermotherapy at different temperatures (37°C, 38°C, 40°C) for a different time interval (1, 3, 5, 7, 10 days) in dry form and when moistened. Marked varietal specificity during germination of seeds after thermotherapy. Thus, the greatest number of seedlings in the emerald variety was obtained after 5 days of thermotherapy (10.0±0), while the Bouquet variety had the best results after 3 days of thermotherapy (9.3±1.2) with moisture. After thermotherapy of dry seeds by 40°C plant material of cultivar Emerald was planted on solid and liquid culture media. The conditions of step sterilization of lettuce seeds for introduction into the culture in vitro were chosen: washing in 96% ethanol, then in 50% aqueous solution of "Whiteness" with the addition of Twin-20, after in sterile distilled water. The nutrient medium for germination of lettuce seeds was used: Gamborg B5 (2% sucrose, 7.0 g/l agar), and the liquid nutrient medium was of that composition. The obtained seedlings were cutted and transferred to medium MS (2% sucrose, 0.1 mg/l ha and 1 mg/l BAP, 3.0 g/l phytogel). The formed shoots for rooting were transferred to the MS medium (2% sucrose, 3.0 g/l phytogel). In the future, lettuce plants will be adapted in vivo and tested for the presence of tomato aspermia virus in the planting material.

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Cytological Assessment of Doubled Haploids in Summer Squash (Cucurbita Pepo L.)

Cited by 4 publications

References 7 publications

The genetics of fruit color in Cucurbita pepo L.

The genetics of fruit color in Cucurbita pepo L.

Advances in Summer Squash (Cucurbita pepo L.) Molecular Breeding Strategies

DEVELOPMENT OF ELEMENTS OF TECHNOLOGY FOR PLANTING MATERIAL OF LETTUCE (<i>LACTUCA SATIVA L.</i>) ON VIRUS-FREE BASIS USING METHODS OF BIOTECHNOLOGY

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