Cytometric bead array: a multiplexed assay platform with applications in various areas of biology

Morgan, Edward T.; Varró, R; Sepulveda, Homero; Ember, Julia A.; Apgar, John R.; Wilson, Jerry D.; Lowe, Larry; Chen, Roy; Shivraj, Lalita; Agadir, Anissa; Campos, Roberto; Ernst, David; Gaur, Amitabh

doi:10.1016/j.clim.2003.11.017

Cited by 455 publications

(308 citation statements)

References 48 publications

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“…During monitoring, subjects were lying on a bed and remained at rest. The frequency-domain parameters of heart rate variability such as low-frequency power Bead Array kit (CBA, BO Bioscience, San Jose, CA, USA), and measurements were made using FACSCalibur flow-cytometry (BO Bioscience) according to the manufacturer's instructions (31)(32). Samples that showed less than the lower limitation of analytical methods for cytokines and Igs, 1/10 the value of minimum values among whole measurable samples, were substituted instead of using "0" or the designation "unmeasurable".…”

Section: Biological Assessmentsmentioning

confidence: 99%

Two Weeks of Permanence in Negatively-Charged Air Conditions Causes Alteration of Natural Killer Cell Function

Takahashi

Otsuki

Mase

et al. 2009

Int J Immunopathol Pharmacol

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The effects of negatively-charged air conditions were analyzed as one of the approaches to improve health and quality of life. We previously reported that the use of a charcoal coating and application of an electric voltage yielded predominantly negatively-charged particles in an experimental room, and that 2.5 hours of living in these conditions caused a slight activation of the immune system (slight elevation of serum interleukin (IL)-2), regulated blood flow, and stabilized the autonomic nervous system when compared with control conditions (no dominance of negatively-charged particles). In this study, we expanded the previous study and placed 15 subjects in negatively-charged air conditions for two weeks during the night and analyzed various biological parameters. Although individual biological reactions differed from subject to subject, natural killer (NK) cell activity increased significantly following living in negatively-charged air conditions. Taken together, the results of the previous investigation and those ofthis study show that repeated elevation ofIL-2 (although it immediately returned to the baseline level) causes chronic and recurrent stimulation to NK cells and results in the steady activation of NK cells. Negatively-charged air particles may be a good tool to improve health and quality of life.The health effects of indoor-air have recently received attention, and some of the adverse effects of indoor-air include sick-building syndrome (SBS) (1-4) and multiple chemical sensitivity syndrome (MCS) (5-8). These diseases are mainly caused by indoor-air aldehydes and volatile organic compounds (VOCs) that affect the human psycho-neuro-endocrineimmune (PNEI) network (9-13). The concept of the PNEI network (PNEI-NW) has evolved in the past few decades and is thought to include a close relationship between immunoregulation and brain functions. Particular emphasis is given to circuits involving immune cell products, the hypothalamuspituitary-adrenal axis, and the sympathetic nervous system (9-13). There is increasing evidence that brain cytokines play an important role in brain

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Section: Biological Assessmentsmentioning

confidence: 99%

Two Weeks of Permanence in Negatively-Charged Air Conditions Causes Alteration of Natural Killer Cell Function

Takahashi

Otsuki

Mase

et al. 2009

Int J Immunopathol Pharmacol

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“…TNFα, interferon-γ, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, and MCP-1 levels in BAL fluid were measured with cytometric bead array kits according to the manufacturer's protocols (BD Biosciences) (30). Specific capture beads for cytokines and chemokines were mixed with 50 μl of BAL fluid or standards, and multiple phycoerythrin-conjugated detection antibodies were added.…”

Section: Cytometric Bead Arraymentioning

confidence: 99%

Cytosolic Phospholipase A2-α Is Necessary for Platelet-activating Factor Biosynthesis, Efficient Neutrophil-mediated Bacterial Killing, and the Innate Immune Response to Pulmonary Infection

Rubin

Downey

Koh

et al. 2005

Journal of Biological Chemistry

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The role of a cytosolic phospholipase A 2 -α (cPLA 2 -α) in neutrophil arachidonic acid release, plateletactivating factor (PAF) biosynthesis, NADPH oxidase activation, and bacterial killing in vitro, and the innate immune response to bacterial infection in vivo was examined. cPLA 2 -α activity was blocked with the specific cPLA 2 -α inhibitor, Pyrrolidine-1 (human cells), or by cPLA 2 -α gene disruption (mice). cPLA 2 -α inhibition or gene disruption led to complete suppression of neutrophil arachidonate release and PAF biosynthesis but had no effect on neutrophil NADPH oxidase activation, FcγII/III or CD11b surface expression, primary or secondary granule secretion, or phagocytosis of Escherichia coli in vitro. In contrast, cPLA 2 -α inhibition or gene disruption diminished neutrophil-mediated E. coli killing in vitro, which was partially rescued by exogenous arachidonic acid or PAF but not leukotriene B 4 . Following intratracheal inoculation with live E. coli in vivo, pulmonary PAF biosynthesis, inflammatory cell infiltration, and clearance of E. coli were attenuated in cPLA 2 -α (−/−) mice compared with wild type littermates. These studies identify a novel * This work was supported by grants from The Physicians of Ontario through The P.S.I. Foundation (Grant 01-12 (to B. B. R.) and 98-049

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“…The fields of application include techniques used in microbiology, like immunoassays of bacterial (9) or viral (10) antigens, or analysis of antiviral/ antibacterial antibodies (7,11), both groups of molecules can be a diagnostic aid for such infections. While this approach matches conventional methods in sensitivity, reproducibility, and simplicity, it seems to have significant advantages by virtue of the possibility for multiplex analysis (5,6,9,(11)(12)(13)(14)(15). Furthermore, sequence-specific capture of PCR-amplified genomic or cDNA sequences allows the detection of single nucleotide polymorphisms (16)(17)(18), and also turns this platform into a possible alternative to microarrays on chips to be used for the characterization of gene expression profiles (19).…”

Section: Introductionmentioning

confidence: 99%

Biological microbeads for flow‐cytometric immunoassays, enzyme titrations, and quantitative PCR

Pataki

Szabó²,

Lantos

et al. 2005

Cytometry Pt A

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BackgroundIntroduction of microbeads into flow‐cytometry has created a new scenario, making quantitative measurement of molecules dispersed in a homogeneous phase, with an extremely wide realm of already realized and potential applications possible. Development of this field has lead to specialized instrumentation and microbead arrays, dedicated to certain applications.MethodsFormaldehyde‐fixed yeast and bacterial cells were conjugated with avidin and applied as microbeads, to establish a simple, convenient, flexible, and inexpensive flow‐cytometric platform for various immunological and biochemical assays.ResultsWe have tested these “biological microbeads” for the simultaneous titration of human α‐fetoprotein (AFP) and human Chorionic Gonadotropin (βhCG) hormone levels, for the titration of proteolytic and nucleolytic (restriction) enzymes, and for quantitative PCR, using biotinylated and fluorescent primers.ConclusionsThe use of biological microbeads for various immunological and biochemical assays has been demonstrated. The flow‐cytometric methods proved to be at least as sensitive as the standard biochemical or immunological tests. For proteinase K activity measurements, a single enzyme molecule in the sample could be detected. The sensitivity, versatility, and low cost of the assays may advance flow‐cytometry to become a central methodological platform in most laboratories. The biological microbeads offer virtually unlimited possibilities for fluorescent labeling (addressing), conjugation of ligand binding molecules, and they are easy to handle and perform well in a multiplex format. © 2005 International Society for Analytical Cytology

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Cytometric bead array: a multiplexed assay platform with applications in various areas of biology

Cited by 455 publications

References 48 publications

Two Weeks of Permanence in Negatively-Charged Air Conditions Causes Alteration of Natural Killer Cell Function

Two Weeks of Permanence in Negatively-Charged Air Conditions Causes Alteration of Natural Killer Cell Function

Cytosolic Phospholipase A2-α Is Necessary for Platelet-activating Factor Biosynthesis, Efficient Neutrophil-mediated Bacterial Killing, and the Innate Immune Response to Pulmonary Infection

Biological microbeads for flow‐cytometric immunoassays, enzyme titrations, and quantitative PCR

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