Cytoprotective Properties of Antioxidant Protein from Curry leaves (Murraya koenigii L.) against Oxidative Stress Induced Damage in Human Erythrocytes

Abstract

Oxidative stress induced by reactive oxygen species (ROS) cause lipid peroxidation at the human erythrocyte membrane with subsequent alterations in the ATPases function. In this study, potential cytoprotective activities of previously reported "Antioxidant Protein from Curry leaves (APC)'' in erythrocytes against reactive oxygen (ROS) species generated by pro-oxidants in vitro. The APC prevented red blood cell lysis induced by pro-oxidants; Fe: As (2:20 μmole), Hydrogen peroxide (0.2 mM), and tertiary butyl hydroperoxide (1 mM) upto 97.5, 82.5 and 63%, respectively. Further, APC prevented Fe:As induced K + leakage in red blood cells up to 95%. The inhibition offered by APC on K + leakage was comparable to inhibition offered by quinine sulphate, a known K + channel blocker. Interestingly, the APC at dose dependently restored Na + K + ATPase and Ca 2+ Mg 2+ ATPase activities of erythrocyte membrane when altered by ROS. The restoration of ATPase activity by APC was two times more than standard antioxidants BHA and α-tocopherol. In conclusion, Curry leaves protein antioxidant is an effective antioxidant in preventing membrane damage and associated functions mediated by reactive oxygen species. It can be further developed as an effective bioprotective antioxidant agent to cellular components.

Antibacterial Effect of Silver Nanoparticles Synthesized Using Murraya koenigii (L.) against Multidrug-Resistant Pathogens

“…The stem bark extract of M. koenigii containing carbazole alkaloids and benzoisofuranone derivatives has potent antibacterial activity against Aspergillus niger , Bacillus subtilis , S. aureus , E. coli , Proteus vulgaris , and Candida albicans [24]. The antioxidant protein from curry leaves have a potent ameliorative effect of DNA damage on human erythrocytes caused by oxidative stress [25].…”

التأثيرات المحتملة للأوراق الکاري والجوز على الفئران المصابة بالسکر المستحث بالألوکسان

“…The elevated level of MDA, serving as a biomarker for lipid peroxidation, found in diabetic control rats is indicative of the inadequacy of antioxidant defenses in combating damage caused by ROS (reactive oxygen species), as indicated by Jelodar et al [51] . Ningappa et al [66] reported that treating diabetic rats with curry leaves can mitigate the adverse effects of oxidative stress, prevent diabetic complications and enhance the heart's mitochondrial function. This is owing to the powerful antioxidant properties of curry leaves, which are beneficial in reducing membrane damage and related activities mediated by ROS.…”

Zinc Oxide Nanoparticles Fabricated and Characterized from Mesophilic Bacteria (Escherichia coli) inhibits the growth of pathological microbes

“…6). The biologically active compounds such as phenolic and flavonoid compounds are main agents for the antioxidant activity they can donate hydrogen to free radicals and break the lipid oxidation chain reaction (Ningappa and Srinivas, 2008;Zahin et al, 2013;Ningappa et al, 2016). The green synthesis of ZnO nanoparticles from the source of mesophilic bacteria (E. coli) and characterized by several techniques such as UV spectroscopy, DLS, zeta potential, FTIR, XRD and SEM for conforming ZnONPs.…”