Cytotoxicity and apoptosis enhancement in breast and cervical cancer cells upon coadministration of mitomycin C and essential oils in nanoemulsion formulations

Al-Otaibi, Waad A.; Alkhatib, Mayson H.; Wali, Abdulwahab Noor

doi:10.1016/j.biopha.2018.07.041

Cited by 58 publications

(25 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, it had been confirmed by in vitro studies that the incorporation of the essential oils into NE had potentiated their inhibitory actions against the cancer cells growth. 14,15 Cells are protected against the overproduction of reactive oxygen species by natural antioxidants. Several studies reported that SAL possessed a powerful antioxidant activity which was explained by the SAL protective effect on the DNA through its antioxidant activity.…”

Section: Discussionmentioning

confidence: 99%

“…13 It has been demonstrated recently that the incorporation of different essential oils into the NEs may induce apoptosis and cytotoxicity in breast cancer cell line (MCF-7) and cervical cancer cell line (HeLa). 14,15 Several studies have demonstrated the antitumor potential of the essential oils against a broad spectrum of cancers. 16 Recently, a large number of research studies have reported the potent effect of salvia (SAL) plant as anticancer, antimicrobial, antimutagenic, antioxidant, and anti-inflammatory.…”

Section: Biochemical Analysis Serum and Urine Analysismentioning

confidence: 99%

See 1 more Smart Citation

Incorporating ifosfamide into salvia oil-based nanoemulsion diminishes its nephrotoxicity in mice inoculated with tumor

2019

Self Cite

View full text Add to dashboard Cite

Introduction: Nephrotoxicity is one of the major side effects of the chemotherapeutic drug, ifosfamide (IFO). In this study, IFO was solubilized in nanoemulsion (NE) containing salvia (SAL) essential oil to investigate its adverse side effects in mice. Methods: One hundred female Swiss albino mice (n = 20/group) were split into five groups. Group I (Normal) received saline solution (0.9% (w/v) NaCl) while groups II-V were intraperitoneally (I.P.) injected with 2.5 × 106 Ehrlich ascetic carcinoma (EAC) cells/mouse. Group II (EAC) represented the untreated EAC-bearing mice. Group III (IFO) was treated with IFO at a dose of 60 mg/kg/d (I.P. 0.3 mL/mouse). Group IV (SAL) was treated with 0.3 mL blank NE-based SAL oil/mouse. Group V (SAL-IFO) was treated with IFO, loaded in 0.3 mL of blank SAL-NE, at a dose of 60 mg/kg/d (I.P. 0.3 mL/mouse). Groups III-V were treated for three consecutive days. Results: There was a double increase in the survival percentage of the SAL-IFO group (60%) relative to the IFO group (30%). Renal damage with the presence of Fanconi syndrome was indicated in the IFO group through a significant elevation in the levels of serum creatinine, blood urea nitrogen, urine bicarbonate, and phosphate in addition to a reduced level of glucose compared to the normal group. On the other hand, the administration of SAL-IFO into the mice reversed this effect. Additionally, the oxidative stress in the kidney tissues of the SAL-IFO group was ameliorated when compared to the IFO group. Conclusion: Incorporating IFO into SAL-NE has protected the kidneys from the damage induced by IFO.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Biochemical Analysis Serum and Urine Analysismentioning

confidence: 99%

Incorporating ifosfamide into salvia oil-based nanoemulsion diminishes its nephrotoxicity in mice inoculated with tumor

2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…In this study, B-NE was successfully formulated by the sonication technique and was loaded with a combination of DTX and TQ. Blank B-NE consisted of the suspended nanodroplets with average diameters of 56.04 ± 4.00 nm with a narrow size distribution, whereas the nanodroplets of (DTX + TQ) B-NE formulation had become significantly swollen in size with 235.00 ± 10.00 nm, indicating the entrapment of the loaded drugs by dispersion within the B-NE matrix 32 . Additionally, the zeta potential values of either blank or drugs loaded B-NEs formulas were approximately below -1 mV with a low net negative charge.…”

Section: Discussionmentioning

confidence: 99%

Incorporation of docetaxel and thymoquinone in borage nanoemulsion potentiates their antineoplastic activity in breast cancer cells

Alkhatib

Bawadud

Gashlan

2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

Combining more than one anticancer agent in a nanocarrier is beneficial in producing a formula with a low dose and limited adverse side effects. The current study aimed to formulate docetaxel (DTX) and thymoquinone (TQ) in borage oil-based nanoemulsion (B-NE) and evaluate its potential in impeding the growth of breast cancer cells. The formulated B-NE and the combination (DTX + TQ) B-NE were prepared by the ultra-sonication method and physically characterized by the dynamic light scattering techniques. The cytotoxicity analyses of (DTX + TQ) B-NE in MCF-7 and MDA-MB-231 cells were evaluated in vitro by using the SRB assay. Cell death mechanisms were investigated in terms of apoptosis and autophagy pathways by flow cytometry. The optimum mean droplet sizes formulated for blank B-NE and the (DTX + TQ) B-NE were 56.04 ± 4.00 nm and 235.00 ± 10.00 nm, respectively. The determined values of the half-maximal inhibitory concentration (IC50) of mixing one-half amounts of DTX and TQ in B-NE were 1.15 ± 0.097 µM and 0.47 ± 0.091 µM in MCF-7 and MDA-MB-231 cells, respectively, which were similar to the IC50 values of the full amount of free DTX in both tested cell lines. The treatment with (DTX + TQ) B-NE resulted in a synergistic effect on both tested cells. (DTX + TQ) B-NE induced apoptosis that was integrated with the stimulation of autophagy. The produced formulation enhances the DTX efficacy against human breast cancer cells by reducing its effective dose, and thus it could have the potential to minimize the associated toxicity.

show abstract

“…In the treatment, HeLa cells were treated at concentrations of 12. [35]. When treated at a dose of 10 µg/mL for 24 h, Fr-MMC endured the nuclear apoptosis in HeLa cells at a lower concentration compared to Gi-MMC.…”

Section: Compoundsmentioning

confidence: 92%

Therapeutic Potential of Natural Products in Treatment of Cervical Cancer: A Review

et al. 2021

View full text Add to dashboard Cite

Cervical cancer is the fourth most common cancer among women worldwide. Though several natural products have been reported regarding their efficacies against cervical cancer, there has been no review article that categorized them according to their anti-cancer mechanisms. In this study, anti-cancerous natural products against cervical cancer were collected using Pubmed (including Medline) and google scholar, published within three years. Their mechanisms were categorized as induction of apoptosis, inhibition of angiogenesis, inhibition of metastasis, reduction of resistance, and regulation of miRNAs. A total of 64 natural products suppressed cervical cancer. Among them, Penicillium sclerotiorum extracts from Cassia fistula L., ethanol extracts from Bauhinia variegate candida, thymoquinone obtained from Nigella sativa, lipid-soluble extracts of Pinellia pedatisecta Schott., and 1′S-1′-acetoxychavicol extracted from Alpinia conchigera have been shown to have multi-effects against cervical cancer. In conclusion, natural products could be attractive candidates for novel anti-cancer drugs.

show abstract

Cytotoxicity and apoptosis enhancement in breast and cervical cancer cells upon coadministration of mitomycin C and essential oils in nanoemulsion formulations

Cited by 58 publications

References 20 publications

Incorporating ifosfamide into salvia oil-based nanoemulsion diminishes its nephrotoxicity in mice inoculated with tumor

Incorporating ifosfamide into salvia oil-based nanoemulsion diminishes its nephrotoxicity in mice inoculated with tumor

Incorporation of docetaxel and thymoquinone in borage nanoemulsion potentiates their antineoplastic activity in breast cancer cells

Therapeutic Potential of Natural Products in Treatment of Cervical Cancer: A Review

Contact Info

Product

Resources

About