Daptomycin Eluted From Calcium Sulfate Appears Effective Against Staphylococcus

Webb, Nathan D.; McCanless, Jonathan D.; Courtney, Harry S.; Bumgardner, Joel D.; Haggard, Warren O.

doi:10.1007/s11999-008-0245-0

Cited by 43 publications

(38 citation statements)

References 17 publications

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“…Like with previously researched sourced CaSO 4 pellets, all pellets exhibited a bolus release of antibiotics on Day 1 [21,26]. However, the total percent release of daptomycin from the naturally sourced CaSO 4 pellets was higher and the concentration of daptomycin released on Day 1 was lower than previously reported (Table 3) [22,27]. The initial daptomycin released from the naturally sourced and synthetic calcium sulfate pellets is closer to initial antibiotics eluted from PMMA beads (Table 3) [13,14,19].…”

Section: Cumulative Daptomycin Mass Released Elution Time (Days)supporting

confidence: 77%

“…The 5 wt% was previously determined using prior research on naturally sourced CaSO 4 pellets [22,27]. Throughout our study, we used aseptic techniques in the fabrication of the pellets.…”

Section: Methodsmentioning

confidence: 99%

“…The synthetic CaSO 4 pellets were made with and without 2.5 mL of sterilized 3% K 2 SO 4 solution, whereas the naturally sourced CaSO 4 pellets were only made with 2.5 mL of 3% K 2 SO 4 solution. Previous research has determined that a concentration of 3% K 2 SO 4 is necessary for the naturally sourced CaSO 4 pellets because the pellets will not retain their shape without the catalyst [27]. The 3% K 2 SO 4 solution was substituted with 1.7 mL of sterile phosphate-buffered saline for the synthetic CaSO 4 pellets with 0% K 2 SO 4 .…”

Section: Methodsmentioning

confidence: 99%

“…We carried out all experiments in four replications (n = 4). Pellets were cast using 3-mm OSTEOSET1 Resorbable Mini-Bead Kits (Wright Medical Technology; Catalog 8400-0511; Lot LB04-0028-27) and following the mixing method used by Wright Medical Technology described in previous research [22,27].…”

Section: Methodsmentioning

confidence: 99%

“…Daptomycin is a semisynthetic antibiotic with activity against Grampositive bacteria and has demonstrated activity against MRSA [6,15]. Daptomycin has been previously researched combined with a calcium sulfate (CaSO 4 ) carrier, and daptomycin reportedly elutes similarly to tobramycin in vitro [22,27]. However, like with many antibiotics used for infected wound treatment, bacterial resistance to daptomycin has been recently reported [26].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Two Sources of Calcium Sulfate for a Local Drug Delivery System: A Pilot Study

Parker

Smith

Courtney

et al. 2011

Clinical Orthopaedics &Amp; Related Research

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Background Local drug delivery has substantial potential to prevent infections compared with systemic delivery. Although calcium sulfate (CaSO 4 ) has been studied for local drug delivery and two types are commercially available, it is unknown whether they differentially release antibiotics. Questions/purposes We determined the differences between two sources of CaSO 4 and the K 2 SO 4 catalyst's presence on the degradation, daptomycin elution, and activity against Staphylococcus aureus. Methods We formed pellets from synthetic and naturally sourced (from gypsum) CaSO 4 and loaded with 5% daptomycin and 3% or 0% K 2 SO 4 . We used in vitro experiments to determine the daptomycin concentration and degradation profiles over 10 days. Turbidity assays were used to evaluate the activity of the daptomycin eluates against S. aureus.

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Section: Cumulative Daptomycin Mass Released Elution Time (Days)supporting

confidence: 77%

“…The 5 wt% was previously determined using prior research on naturally sourced CaSO 4 pellets [22,27]. Throughout our study, we used aseptic techniques in the fabrication of the pellets.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Evaluation of Two Sources of Calcium Sulfate for a Local Drug Delivery System: A Pilot Study

Parker

Smith

Courtney

et al. 2011

Clinical Orthopaedics &Amp; Related Research

Self Cite

View full text Add to dashboard Cite

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Calcium sulfate: Properties and clinical applications

2008

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Calcium sulfate (CS) has enjoyed a longer history of clinical use than most currently available biomaterials. It is well-tolerated when used to fill bone defects and undergoes rapid and complete resorption without eliciting a significant inflammatory response. The raw material from which it is made is relatively inexpensive and abundant. In addition, CS can be used as a vehicle to deliver antibiotics, pharmacologic agents, and growth factors. It has found wide use in orthopedics and dentistry, and has been used in a variety of clinical applications, including the periodontal defect repair, the treatment of osteomyelitis, sinus augmentation, and as an adjunct to dental implant placement. Despite these advantages, the material has not enjoyed the popularity of many other regenerative materials, although there has been a recent resurgence of interest in the material. This review examines the properties and clinical applications of CS, with an emphasis on dental applications of the material. Limitations of the material are discussed as well as suggestions for future research.

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