Daya Antibakteri Filtrat Asam Laktat dan Bakteriosin Lactobacillus bulgaricus KS1 dalam Menghambat Pertumbuhan Klebsiella pneumoniae Strain ATCC 700603, CT1538, dan S941

Fauziah, Prima Nanda; Nurhajati, Jetty; Chrysanti,

doi:10.15395/mkb.v47n1.395

Cited by 7 publications

(8 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, previous studies showed that the highest growth was also at pH 7 [10]. However, some showed that the best LAB growth was at pH 6.2 [5]. In this study, LAB which grew at pH 6 also showed quite optimal results and was not significantly different from treatment at pH 7.…”

Section: Growth Of Lab Strain Pr 43lmentioning

confidence: 41%

“…LAB grows naturally in traditional fermented food products such as peda fish, and some obtained are Lactobacillus, Pediococcus, Enterococcus, Weisella, and Leuconostoc species [3]. In addition, it has the potential of being substituted as antibiotics and biopreservatives in traditional fermented foods [4], and its inappropriate consumption can cause biological resistance [5]. Therefore, the LAB is chosen as one of the alternatives to limit the use of antibiotics and not cause side effects on human health.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Effect of Temperatures and pH on Bacteriocin Activity of Lactic Acid Bacteria Strain Pr 4.3L From Peda Fish

Amarantini¹,

Prakasita²,

Cahyani³

2021

Advances in Social Science, Education and Humanities Research

View full text Add to dashboard Cite

Lactic acid bacteria (LAB) produce bacteriocin, which is potentially administered as biopreservative. It is important to study bacteriocin continuously from an applied perspective. However, isolate LAB strain Pr 4.3L obtained from peda fish are known to produce antimicrobial compounds such as bacteriocin. Therefore, it is important to optimize the production of bacteriocin from these strains by examining the effect of incubation temperatures and pH on the activity of LAB strain Pr 4.3L. The biomass of lactic acid bacteria strain Pr 4.3L was grown in MRS broth medium with pH conditions of 4-8 which was adjusted using 1 N HCl and NaOH. Furthermore, the culture was incubated at 30 and 37°C for 36 hours, and the bacteriocin activity was tested for its inhibition against pathogenic bacteria (S.aureus ATCC 25923, S.typhi BPE 122.4 CCA and S.typhi NCTC 786) using agar well diffusion method. The treatment at 30°C on MRS broth medium with a pH of 7 is the best growth condition for bacteriocin production. Under these conditions, antimicrobial and bacteriocin activities are more inhibited than other treatments. This inhibition showed a broad-spectrum against indicator bacteria (S.aureus ATCC 25923, S.typhi BPE 122.4 CCA and S.typhi NCTC 786). Therefore, the results provided useful information on the potential of bacteriosinogenic LAB as a natural preservative.

show abstract

Section: Growth Of Lab Strain Pr 43lmentioning

confidence: 41%

Section: Introductionmentioning

confidence: 99%

The Effect of Temperatures and pH on Bacteriocin Activity of Lactic Acid Bacteria Strain Pr 4.3L From Peda Fish

Amarantini¹,

Prakasita²,

Cahyani³

2021

Advances in Social Science, Education and Humanities Research

View full text Add to dashboard Cite

show abstract

“…Bakteriosin adalah zat antimikroba yang berupa polipeptida, atau senyawa mirip protein. Umumnya bakteriosin dapat menyebabkan kebocoran sel yang dapat mengakibatkan kematian sel bakteri (Fauziah et al, 2014). Akan tetapi karena dalam penelitian ini tidak dilakukan pengujian untuk deteksi produksi peroksida atau bakteriosin maka belum dapat dipastikan senyawa non asam dengan aktivitas antibakteri yang dihasilkan ke-tiga isolat BAL apakah peroksida atau bakteriosin atau senyawa lain.…”

Section: Hasil Dan Pembahasanunclassified

Aktifitas Antibakteri Isolat Bakteri Asam Laktat Saluran Pencernaan Kuda Laut (Hippocampus kuda Bleeker, 1852) Terhadap Vibrio harveyi

Sandi

Subagiyo

2022

J. Kel. Trop.

View full text Add to dashboard Cite

Seahorses (Hippocampus kuda) have high economic value in global trade. Therefore, to meet market demand, cultivation efforts have been carried out. Vibriosis caused by Vibrio harveyi is a disease that is often reported to affect seahorses. In this study, lactic acid bacteria were isolated from the digestive tract of seahorses, and were selected based on their antibacterial activity against V, harveyi. Antibacterial activity test was carried out by agar diffusion method. Isolates with antibacterial activity were identified based on their morphological and biochemical characteristics. The results of this study obtained 3 (three) LAB isolates that were active against V. harveyi from a total of 45 isolates, namely SPKL 34, SPKL 08 and SPKL 58. The SPKL 34 isolate had the highest antibacterial activity. The three isolates were identified as lactobacillus sp Kuda laut (Hippocampus kuda) memiliki nilai ekonomi tinggi sebagai salah satu jenis komoditas perdagangan global. Oleh karena itu untuk memenuhi permintaan pasar diantaranya telah dilakukan upaya budidaya. Vibriosis yang disebabkan oleh Vibrio harveyi merupakan penyakit yang sering dilaporkan menyerang Kuda Laut. Pada penelitian telah dilakukan pencarian jenis-jenis bakteri asam laktat (BAL) yang berasal dari saluran pencernaan Kuda Laut yang memiliki aktivitas antibakteri terhadap V. harveyi. Uji aktivitas antibakteri dilakukan dengan metode difusi agar. Isolat yang memiliki aktivitas antibakteri diidentifikasi berdasarkan karakteristik morfologi dan biokimia. Hasil penelitian ini memperoleh 3 (tiga) isolat BAL yang aktif terhadap V. harveyi dari total 45 isolat yaitu SPKL 34, SPKL 08 dan SPKL 58. Isolat SPKL 34 memiliki aktivitas yang paling tinggi yaitu dengan rerata luas zona hambat sebesar 4,14±0,60 mm. Hasil identifikasi menunjukkan ketiga isolat merupakan Lactobacillus sp. dengan tingkat kemiripan >80%.

show abstract

“…Sulistiani (2017) Bakteriosin yaitu peptida dan aktif melawan bakteri lain yang disintesis di ribosom, berspektrum luas dan berspektrum sempit. Bakteriosin adalah protein dan bersifat antibakteri serta mampu dalam penghambatan pertumbuhan bakteri patogen yang diekskresikan oleh bakteri probiotik (Fauziah et al, 2014). Mekanisme bakterisidal bakteriosin bervariasi, dapat pembentukan lubang, terdegradasinya DNA sel, atau sintesis peptidoglikan yang terhambat (Todorov et al, 2011), sedangkan menurut Yi et al, (2016) mekanisme bakteriosin dapat melalui penghambatan replikasi DNA serta pengaruhnya terhadap permeabilitas membran sel.…”

Section: Pembahasanunclassified

Uji Aktivitas Antibakteri Isolat Bakteri yang Diisolasi dari Umbi Porang (Amorphophallus muelleri) Terhadap Escherichia coli FNCC 0091 dan Staphylococcus aureus FNCC 0047

Savira

Trimulyono²

2021

LenteraBio

View full text Add to dashboard Cite

Escherichia coli dan Staphylococcus aureus termasuk dalam bakteri patogen. Escherichia coli merupakan penyebab infeksi penyakit diare serta Staphylococcus aureus dapat menyebabkan infeksi kulit pada manusia. Oleh sebab itu, diperlukan pengendalian menggunakan bahan alami, salah satunya isolat bakteri dari umbi porang (Amorphophallus muelleri) yang bersifat antibakteri. Penelitian dilakukan dengan mengisolasi bakteri pada umbi porang (Amorphophallus muelleri) yang memiliki aktivitas antibakteri terhadap penghambatan pertumbuhan Escherichia coli FNCC 0091 serta Staphylococcus aureus FNCC 0047. Umbi porang sebelum perlakuan difermentasikan selama 5 hari di udara terbuka. Proses isolasi menggunakan metode pour plate dilanjutkan dengan purifikasi koloni menggunakan metode streak plate. Uji aktivitas antibakteri dilakukan dengan 3 perlakuan yaitu kontrol negatif (akuades steril), isolat bakteri umbi porang, dan kontrol positif (kloramfenikol) dengan 3 pengulangan menggunakan metode sumuran. Isolat yang didapat berjumlah 9 isolat yang enam di antaranya berpotensi sebagai antibakteri terhadap pertumbuhan E. coli dan S. aureus. Isolat P2 menunjukkan aktivitas antibakteri tertinggi terhadap E. coli sedangkan P5 terhadap S. aureus diperoleh zona hambat secara berurutan yaitu 2,43 ± 0,40 cm dan 3,66 ± 0,57 cm.

show abstract

Daya Antibakteri Filtrat Asam Laktat dan Bakteriosin Lactobacillus bulgaricus KS1 dalam Menghambat Pertumbuhan Klebsiella pneumoniae Strain ATCC 700603, CT1538, dan S941

Abstract: AbstrakLactobacillus bulgaricus menghasilkan asam laktat dan bakteriosin yang memiliki efek farmakologik, di antaranya sebagai antibakteri. Klebsiella pneumoniae penyebab pneumonia masih merupakan masalah kesehatan masyarakat di negara tropis.

Cited by 7 publications

References 12 publications

The Effect of Temperatures and pH on Bacteriocin Activity of Lactic Acid Bacteria Strain Pr 4.3L From Peda Fish

The Effect of Temperatures and pH on Bacteriocin Activity of Lactic Acid Bacteria Strain Pr 4.3L From Peda Fish

Aktifitas Antibakteri Isolat Bakteri Asam Laktat Saluran Pencernaan Kuda Laut (Hippocampus kuda Bleeker, 1852) Terhadap Vibrio harveyi

Uji Aktivitas Antibakteri Isolat Bakteri yang Diisolasi dari Umbi Porang (Amorphophallus muelleri) Terhadap Escherichia coli FNCC 0091 dan Staphylococcus aureus FNCC 0047

Contact Info

Product

Resources

About