Daya Hambat Minyak Hati Ikan Hiu Terhadap Pertumbuhan Bakteri Porphyromonas gingivalis

Agustina, Felisitas Maria; Mulawarmanti, Dian; Wedarti, Yoifah Rizka

doi:10.30649/denta.v9i2.9

Cited by 3 publications

(4 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…They were both tested on both gramnegative bacteria, for example; Escherichia coli and Pseudomonas aeruginosa, in addition to gram-positive bacteria such as Staphylococcus aureus and Streptococcus pneumoniae. Similarly, another investigation undertaken by Agustina (2015) 14 showed that shark liver oil has inhibitory properties in relation to Porphyromonas gingivalis bacteria. Alkylglycerol can bind to phospholipid cell membrane changing its structure to membrane fluidity and antioxidants and protecting the structure and function of membranes in white blood cells and macrophages.…”

Section: Discussionmentioning

confidence: 85%

“…12 Centrophorus moluccensis is a type of shark whose liver oil is commonly extracted for subsequent use for medicinal purposes. 13 Previous research conducted by Agustina (2015) 14 indicated that shark liver oil has inhibitory properties in relation to Porphyromonas gingivalis bacteria. The alkylglycerol contained in sharks can even protect the structure and function of white blood cell membranes and macrophages.…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

The effects of shark liver oil on fibroblasts and collagen density in the periodontal ligaments of Wistar rats induced with Porphyromonas gingivalis

et al. 2019

Self Cite

View full text Add to dashboard Cite

Background: Periodontitis is an infection in tooth-supporting tissues caused by a specific microorganism, Porphyromonas Gingivalis (PG), which can trigger collagen destruction. Generally, periodontal therapy employs a combination of mechanical (scaling root planning/SRP) and chemical (antibiotics) remedies, the latter of which can cause bacterial resistance. On the other hand, shark liver oil contains active natural ingredients such as alkylglycerols, squalene, squalamine, and omega-3, which have antibacterial and antioxidant effects. Purpose: This study aims to determine the impact of shark liver oil on fibroblasts and collagen density in the periodontal ligament of Wistar rats induced with PG. Methods: This study represents a laboratory experiment with post-test only control group design. The research subjects consisted of 35 Wistar rats divided into five groups, namely; a negative control group (K-); a positive control group with PG induction (K+); and three treatment groups induced with PG and shark liver oil once a day for seven days at varying doses of 0.2 g/gBB (P1), 0.3 g/gBB (P2), and 0.4 g/gBB (P3). Following treatment, the subjects were euthanized. The number of fibroblasts was then histologically examined with Hematoxylin Eosin (HE). Meanwhile, the collagen density was histologically analyzed with Masson’s Tricrome. Fibroblast cells were observed through a microscope at 400x magnification. Data was statistically analyzed with a one-way ANOVA and post hoc LSD. Collagen Density scoring was then performed. The results were analyzed with a non-parametric Kruskal-Wallis test (p=0.05), and subsequently with a Mann-Whitney U test (p<0.05). Results: The number of fibroblasts in the periodontal ligament areas of each group were 18.6 ± 1.21 for K-; 12 ± 1.26 for K;16.8 ± 1.72 for P1; 17.1 ± 1.94 for P2; and 23.16 ± 2.78 for P3. The results also indicated that there were significant differences between K- with K+ and P3, K+ with P1, P2, and P3, as well as P3 with P1 and P2. However, there was no significant difference between K- and P1 and P2 or P1 and P2. The results showed that collagen density in the negative control group did not significantly decrease compared to that in the positive control group in which PG was induced. Meanwhile, collagen density in all three treatment groups following doses of 0.2 g/gBB, 0.3 g/gBB, and 0.4 g/gBB being administered significantly increased compared to that in the negative control group and the positive control group subjected to PG induction. Conclusion: Shark liver oil can significantly increase fibroblast cells and collagen density in the periodontal ligament of Wistar rats induced with PG.

show abstract

Section: Discussionmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 98%

The effects of shark liver oil on fibroblasts and collagen density in the periodontal ligaments of Wistar rats induced with Porphyromonas gingivalis

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…Oleh karena itu, diperlukan terapi alternatif untuk mengobati penyakit periodontal tanpa efek samping. [6] Kemampuan daya hambat bakteri pada biji kelor sudah banyak dilaporkan, salah satunya dari penelitian Christian dkk (2015) menyimpulkan bahwa terdapat efek antibateri ekstrak daun dan biji kelor terhadap bakteri Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. [7] Bakteri lain yang ada di dalam rongga mulut adalah Porphyromonas gingivalis.…”

Section: Pendahuluanunclassified

“…[10], [11] Menurut Sendy dkk (2014) menyatakan bahwa terdapat daya antibakteri ekstrak daun sirih merah (Pipper crocatum) terhadap Porphyromonas gingivalis. Kartasapoetra (1992), menyatakan dari hasil kromatogram, diketahui daun sirih merah mengandung minyak atsiri yang sepertiga bagiannya terdiri atas fenol-fenol yang khas yang disebut betelfenol atau aseptosol, alkaloid, flavonoid dan polevenolad sedangkan kavikol yang khasiatnya bakterisidal yang merupakan eugenol methylester diperkirakan khasiatnya sama dengan eugenol sebagai bahan antiseptik, [15], [16] Moringa stenopetala adalah tanaman asli dari Kenya dan Ethiopia. Moringa stenopetala menghasilkan dedaunan yang dapat dimakan, buah, dan biji yang kaya akan asam amino esensial, vitamin, dan mineral.…”

Section: Mean ± Sd P-valueunclassified

Efektivitas Daya Hambat Antibakteri Ekstrak Metanol Biji Kelor (Moringa Oleifera) terhadap Pertumbuhan Porphyromonas Gingivalis (in vitro)

Irawati¹,

Mattulada

Wijaya

et al. 2021

SMJ

View full text Add to dashboard Cite

Pendahuluan: Periodontitis merupakan suatu penyakit inflamasi destruktif pada jaringan penyangga gigi yang disebabkan oleh mikroorganisme spesifik Ada tiga bakteri utama penyebab penyakit periodontal yang banyak ditemukan pada plak subgingiva, ketiga bakteri tersebut adalah Porphyromonas gingivalis, Treponema denticola,Bacteroides forsythus. Kandungan antibakteri alami pada tanaman kelor yang dapat dimanfaatkan sebagai terapi alternatif dari penyakit yang disebabkan oleh bakteri gram negatif, salah satunya adalah penyakit periodontal.Tujuan: Untuk mengetahui daya hambat antibakteri ekstrak metanol biji kelor(Moringa oleifera) terhadap pertumbuhan Porphyromonas gingivalis. Bahan dan Metode: Penelitian ini menggunakan metode eksperimental laboratorium dengan bentuk Posttest Only Control Design. Pengambilan sampel dengan Purposive Sampling menggunakan 8 perlakuan dengan ekstrak metanol biji kelor konsentrasi 2,5%;5%;10%;15%;20%;25%, kontrol positif dan negatif. Uji statistic menggukanakan One Way Anova. Hasil: Hasil penelitian menunjukkan daya hambat antibakteri terlihat pada seluruh konsentrasi ektrak metanol biji kelor namun pada tes statistik menunjukkan bahwa tidak terdapat perbedaan signifikan antara konsentrasi 2,5%; 5%; 10% dan 15%;20%;25% Kesimpulan: Ekstrak metanol biji kelor (Moringa oleifera) memiliki daya hambat antibakteri terhadap pertumbuhan Porphyromonas Gingivalis

show abstract

Impact of virgin coconut oil (VCO) on probiotic Lactobacillus delbrueckii subsp. bulgaricus

Miksusanti

Herlina

Untari

et al. 2023

The 5th INTERNATIONAL CONFERENCE ON AGRICULTURE AND LIFE SCIENCE 2021 (ICALS 2021): “Accelerating Transformation in Industrial

View full text Add to dashboard Cite

Daya Hambat Minyak Hati Ikan Hiu Terhadap Pertumbuhan Bakteri Porphyromonas gingivalis

Abstract: ABSTRACT

Cited by 3 publications

References 3 publications

The effects of shark liver oil on fibroblasts and collagen density in the periodontal ligaments of Wistar rats induced with Porphyromonas gingivalis

The effects of shark liver oil on fibroblasts and collagen density in the periodontal ligaments of Wistar rats induced with Porphyromonas gingivalis

Efektivitas Daya Hambat Antibakteri Ekstrak Metanol Biji Kelor (Moringa Oleifera) terhadap Pertumbuhan Porphyromonas Gingivalis (in vitro)

Impact of virgin coconut oil (VCO) on probiotic Lactobacillus delbrueckii subsp. bulgaricus

Contact Info

Product

Resources

About