Dental Pulp Stem Cells and Tissue Engineering Strategies for Clinical Application on Odontoiatric Field

Zavan, Barbara; Bressan, Eriberto; Brunello, Giulia; Gardin, Chiara; Ferrarese, Nadia; Ferroni, Letizia; Stellini, Edoardo

doi:10.5772/24871

Cited by 7 publications

(9 citation statements)

References 31 publications

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“…Figure shows the spindle‐shape morphology of SHED seeded on TCP which was similar to the morphology of SHED reported by other researchers …”

Section: Resultssupporting

confidence: 87%

“…Several cell types such as bone marrow mesenchymal stem cells (BMMSCs), mesenchymal stem cells (MSCs), Schwann cells, co‐culture of dorsal root ganglia and Schwann cells and neural stem cells (NSCs) have been used for the improvement of peripheral nerve regeneration through their seeding in conventional nerve guides and the results showed faster improvement of injured nerve and positive impact of these cells on nerve regeneration . However, the use of NSCs from other tissues is considered invasive, and the clinical use of BMMSCs is also limited due to its painful recovery as well as the availability of limited number of harvested cells from BMMSCs …”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have shown that SHED are a source of multipotent stem cells with ability to differentiate into different cell types including odontoblasts, chondrocytes, endothelocytes, adipocytes, smooth muscle cells, and osteoblasts …”

Section: Discussionmentioning

confidence: 99%

“…Every child loses primary teeth, which provides the gold opportunity to recover and store this source of stem cells (SHED) to regenerate future injuries . Biological properties of these cells such as multipotency, high proliferation rates and accessibility make them important in regenerative medicine …”

Section: Introductionmentioning

confidence: 99%

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In vivointegration of poly(ε-caprolactone)/gelatin nanofibrous nerve guide seeded with teeth derived stem cells for peripheral nerve regeneration

Beigi

Ghasemi‐Mobarakeh

Prabhakaran

et al. 2014

J. Biomed. Mater. Res.

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Artificial nanofiber nerve guides have gained huge interest in bridging nerve gaps and associated peripheral nerve regeneration due to its high surface area, flexibility and porous structure. In this study, electrospun poly (ε-caprolactone)/gelatin (PCL/Gel) nanofibrous mats were fabricated, rolled around a copper wire and fixed by medical grade adhesive to obtain a tubular shaped bio-graft, to bridge 10 mm sciatic nerve gap in in vivo rat models. Stem cells from human exfoliated deciduous tooth (SHED) were transplanted to the site of nerve injury through the nanofibrous nerve guides. In vivo experiments were performed in animal models after creating a sciatic nerve gap, such that the nerve gap was grafted using (i) nanofiber nerve guide (ii) nanofiber nerve guide seeded with SHED (iii) suturing, while an untreated nerve gap remained as the negative control. In vitro cell culture study was carried out for primary investigation of SHED-nanofiber interaction and its viability within the nerve guides after 2 and 16 weeks of implantation time. Walking track analysis, plantar test, electrophysiology and immunohistochemistry were performed to evaluate functional recovery during nerve regeneration. Vascularization was also investigated by hematoxilin/eosine (H&E) staining. Overall results showed that the SHED seeded on nanofibrous nerve guide could survive and promote axonal regeneration in rat sciatic nerves, whereby the biocompatible PCL/Gel nerve guide with cells can support axonal regeneration and could be a promising tissue engineered graft for peripheral nerve regeneration.

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“…Figure shows the spindle‐shape morphology of SHED seeded on TCP which was similar to the morphology of SHED reported by other researchers …”

Section: Resultssupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In vivointegration of poly(ε-caprolactone)/gelatin nanofibrous nerve guide seeded with teeth derived stem cells for peripheral nerve regeneration

Beigi

Ghasemi‐Mobarakeh

Prabhakaran

et al. 2014

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

show abstract

“…Kaedah pemencilan bagi sel stem pulpa gigi (DPSCs) (Barbara et al 2011;Djouad et al 2010;Nadig 2009). The soft tissue residing in the inner structure of teeth known as dental pulp is a non-invasive source of adult stem cells.…”

mentioning

confidence: 99%