Design and manufacture of neural tissue engineering scaffolds using hyaluronic acid and polycaprolactone nanofibers with controlled porosity

Entekhabi, Elahe; Nazarpak, Masoumeh Haghbin; Moztarzadeh, Fathollah; Sadeghi, Ali

doi:10.1016/j.msec.2016.06.078

Cited by 109 publications

(72 citation statements)

References 52 publications

(54 reference statements)

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“…Moreover, the morphology of the sample surface, before and after immersion, was characterized by SEM, as explained earlier . Also, as the scaffolds might be chemically affected by degradation, ATR‐FTIR spectra were used to investigate the possible chemical changes in the scaffolds structure . Furthermore, DSC was used to analyze the morphological changes of degraded electrospun fibrous mats based on the same protocol used before…”

Section: Methodsmentioning

confidence: 99%

Preparation and Characterization of Electrospun Blend Fibrous Polyethylene Oxide:Polycaprolactone Scaffolds to Promote Cartilage Regeneration

et al. 2020

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Fibrous scaffolds can be used to mimic the structure of cartilage extracellular matrix aiming at cartilage regeneration through optimal utilization of such scaffolds and chondrogenic cells. Herein, different types of fibrous structures are manufactured through electrospinning of blends of polyethylene oxide (PEO) and polycaprolactone (PCL), and the physical and chemical characteristics of the produced fiber mats are investigated. The amount of PEO influenced hydrophilicity, biodegradability, and mechanical properties of the blend fibers. To assess the cytotoxicity and biocompatibility of the scaffolds as well as the effect of fiber orientation, in vitro cell culture studies with a chondrogenic cell line (ATDC5) are conducted. The results show no cytotoxicity of the developed fibrous structures. A promising fibrous scaffold technology is presented with potential applications in cartilage tissue engineering.

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Section: Methodsmentioning

confidence: 99%

Preparation and Characterization of Electrospun Blend Fibrous Polyethylene Oxide:Polycaprolactone Scaffolds to Promote Cartilage Regeneration

et al. 2020

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“…In contrast, U373 cells did not display a difference in migration rate or adhesion behavior when seeded on PCL/collagen-blended and PCL fiber scaffolds. SH-SY5Y neuroblastoma cells had an increased metabolic activity when seeded on PCL/hyaluronan-blended fibers over PCL fibers (Entekhabi et al, 2016). Electrospun fibers made from PCL blended with gelatin increased C17.2 NSC neurite length over PCL fiber scaffold controls (Ghasemi-Mobarakeh et al, 2008).…”

Section: Fiber Materials Compositionmentioning

confidence: 98%

Microscale Architecture in Biomaterial Scaffolds for Spatial Control of Neural Cell Behavior

Meco

Lampe

2018

Front. Mater.

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Biomaterial scaffolds mimic aspects of the native central nervous system (CNS) extracellular matrix (ECM) and have been extensively utilized to influence neural cell (NC) behavior in in vitro and in vivo settings. These biomimetic scaffolds support NC cultures, can direct the differentiation of NCs, and have recapitulated some native NC behavior in an in vitro setting. However, NC transplant therapies and treatments used in animal models of CNS disease and injury have not fully restored functionality. The observed lack of functional recovery occurs despite improvements in transplanted NC viability when incorporating biomaterial scaffolds and the potential of NC to replace damaged native cells. The behavior of NCs within biomaterial scaffolds must be directed in order to improve the efficacy of transplant therapies and treatments. Biomaterial scaffold topography and imbedded bioactive cues, designed at the microscale level, can alter NC phenotype, direct migration, and differentiation. Microscale patterning in biomaterial scaffolds for spatial control of NC behavior has enhanced the capabilities of in vitro models to capture properties of the native CNS tissue ECM. Patterning techniques such as lithography, electrospinning and three-dimensional (3D) bioprinting can be employed to design the microscale architecture of biomaterial scaffolds. Here, the progress and challenges of the prevalent biomaterial patterning techniques of lithography, electrospinning, and 3D bioprinting are reported. This review analyzes NC behavioral response to specific microscale topographical patterns and spatially organized bioactive cues.

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“…Likewise, Entekhabi et al combined Hyaluronic acid (one of the major components of the ECM) with PCL to improve hydrophilicity and biological properties of scaffold for nerve tissue engineering [115]. In other work, PAN et al developed NGCs with PPy-PCL-nanoyarns as fillers to evaluate their effects on nerve regeneration.…”

Section: Pcl Polymermentioning

confidence: 99%

Tailoring synthetic polymeric biomaterials towards nerve tissue engineering: a review

Amani

Kazerooni

Hassanpoor

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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The nervous system is known as a crucial part of the body and derangement in this system can cause potentially lethal consequences or serious side effects. Unfortunately, the nervous system is unable to rehabilitate damaged regions following seriously debilitating disorders such as stroke, spinal cord injury and brain trauma which, in turn, lead to the reduction of quality of life for the patient. Major challenges in restoring the damaged nervous system are low regenerative capacity and the complexity of physiology system. Synthetic polymeric biomaterials with outstanding properties such as excellent biocompatibility and non-immunogenicity find a wide range of applications in biomedical fields especially neural implants and nerve tissue engineering scaffolds. Despite these advancements, tailoring polymeric biomaterials for design of a desired scaffold is fundamental issue that needs tremendous attention to promote the therapeutic benefits and minimize adverse effects. This review aims to (i) describe the nervous system and related injuries. Then, (ii) nerve tissue engineering strategies are discussed and (iii) physiochemical properties of synthetic polymeric biomaterials systematically highlighted. Moreover, tailoring synthetic polymeric biomaterials for nerve tissue engineering is reviewed.

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Design and manufacture of neural tissue engineering scaffolds using hyaluronic acid and polycaprolactone nanofibers with controlled porosity

Cited by 109 publications

References 52 publications

Preparation and Characterization of Electrospun Blend Fibrous Polyethylene Oxide:Polycaprolactone Scaffolds to Promote Cartilage Regeneration

Preparation and Characterization of Electrospun Blend Fibrous Polyethylene Oxide:Polycaprolactone Scaffolds to Promote Cartilage Regeneration

Microscale Architecture in Biomaterial Scaffolds for Spatial Control of Neural Cell Behavior

Tailoring synthetic polymeric biomaterials towards nerve tissue engineering: a review

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