Detection and Determination of Tetracyclines in Imported Frozen Shrimp by Bioassay and HPLC

Kamakura, Kazumasa; Hasegawa, Masumi; Koiguchi, Satoshi; Goto, I; Shiraishi, Syogo; Hirata, Kenshi; Yamana, Takayoshi; Tonogai, Yasuhide

doi:10.3358/shokueishi.35.310

Cited by 3 publications

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“…Coded samples without product labels were analyzed at the National Agro-Forestry Fisheries Quality Assurance Department (NAFIQAD) laboratory in Can Tho, Vietnam which is ISO/IEC 17025 accredited [ 5 ]. Solutions of antimicrobial products were analyzed using chromatographic separation conditions and detection parameters as described previously [ 6 – 10 ]. Ultra High Performance Liquid Chromatography Mass Spectrometry (UPLC-MS/MS) was used for sulfamethoxazole (SMX); sulfadiazine (SDZ); sulfamethazine (SMZ) and trimethoprim (TMP) [ 6 ]; amoxicillin (AMX) and cefalexin (LEX) [ 7 ]; and ciprofloxacin (CIP) [ 8 ] whereas concentrations of florfenicol (FFC) [ 9 ] and doxycycline (DOX) [ 10 ] products were analyzed by High Performance Liquid Chromatography with UV detection (HPLC-UV).…”

Section: Methodsmentioning

confidence: 99%

Quality of Antimicrobial Products Used in Striped Catfish (Pangasianodon hypophthalmus) Aquaculture in Vietnam

et al. 2015

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Antimicrobial usage is common in Asian aquaculture. This study aimed to determine the quality of antimicrobial products used by Vietnamese striped catfish (Pangasianodon hypophthalmus) farmers. Twenty one antimicrobial products (11 products contained a single antimicrobial and 10 products contained a mixture of two different antimicrobials) commonly used by catfish farmers were obtained from so-called chemical shops located in the Mekong Delta, Vietnam. Ultra High Performance Liquid Chromatography Mass Spectrometry was used to analyze concentration of sulfonamides, trimethoprim, amoxicillin, cefalexin and ciprofloxacin whereas concentrations of florfenicol and doxycycline were analyzed by High Performance Liquid Chromatography with UV detection. Results revealed that only 4/11 products with a single antimicrobial and 2/10 products with a mixture of antimicrobials contained active substances within ±10% of the concentration declared on the product label. Two products with antimicrobial mixtures did not contain any of the declared antimicrobials. Comparing two batches, analysis of 11 products revealed that only one product contained a concentration of active compound that varied with less than 10% in both batches. Several product labels provided inadequate information on how to calculate therapeutic dosage and further stated withdrawal time despite lack of pharmacokinetic data on the antimicrobials in catfish. There is an urgent need to strengthen approval procedures and in particular regularly to monitor the quality of antimicrobials used in Vietnamese aquaculture.

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Section: Methodsmentioning

confidence: 99%

Quality of Antimicrobial Products Used in Striped Catfish (Pangasianodon hypophthalmus) Aquaculture in Vietnam

et al. 2015

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“…Several techniques have been employed to assay tetracycline: for example, microbiological assay (2,5,6), continuous-flow chemiluminometric method (7), spectrophotometry and flow injection method (8), flow injection with chemiluminescence detection (9), and metal chelate affinity chromatography (10). The bioassay method has its limitations in both selectivity and efficiency.…”

Section: Introductionmentioning

confidence: 99%

Determination of Tetracycline and Streptomycin in Mixed Fungicide Products by Capillary Zone Electrophoresis

Hsiao

2001

J. Agric. Food Chem.

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A method of capillary zone electrophoresis (CZE) was used to determine tetracycline and streptomycin content in commercial agriculture products. The results indicated that this method was capable of analyzing the mixed fungicide in formulated products with instrument detection limit (IDL) of 0.50 microg/mL and a method detection limit (MDL) of 0.52 microg/mL for tetracycline, and IDL of 1.00 microg/mL and MDL of 1.22 microg/mL for streptomycin. Precision expressed by relative standard deviation (RSD) ranged from 1.44 to 4.37% of tetracycline and 1.00 to 4.20% of streptomycin. Recoveries were in the region of 98.2-102.5% for tetracycline and 95.3--103.0% for streptomycin. The low detection limit, the low RSD values, and the high percentage of recovery confirmed that the CZE technique is a sensitive and selective method. And the CZE method can analyze both tetracycline and streptomycin at the same time without complicated extraction and further derivative reaction.

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Quality of antimicrobial products used in white leg shrimp ( Litopenaeus vannamei ) aquaculture in Northern Vietnam

et al. 2018

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Detection and Determination of Tetracyclines in Imported Frozen Shrimp by Bioassay and HPLC

Cited by 3 publications

References 0 publications

Quality of Antimicrobial Products Used in Striped Catfish (Pangasianodon hypophthalmus) Aquaculture in Vietnam

Quality of Antimicrobial Products Used in Striped Catfish (Pangasianodon hypophthalmus) Aquaculture in Vietnam

Determination of Tetracycline and Streptomycin in Mixed Fungicide Products by Capillary Zone Electrophoresis

Quality of antimicrobial products used in white leg shrimp ( Litopenaeus vannamei ) aquaculture in Northern Vietnam

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