Detection and discrimination of barley- and wheat-specific forms of <i>Wheat dwarf virus</i> in Poland

Trzmie, K.

doi:10.1556/0806.46.2018.11

Cited by 6 publications

(7 citation statements)

References 22 publications

(26 reference statements)

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“…In Poland WDV was found for the first time in 1999 (Jeżewska 2001). The next studies confirmed WDV infections of wheat, barley and triticale plants in different parts of Poland (Jeżewska et al 2010) as well as the presence of both WDV-B and WDV-W forms (Trzmiel 2018).…”

Section: Rna Isolationsupporting

confidence: 65%

“…The results presented herein revealed the common presence of BYDV-SGV in tested samples. Our data, in comparison to a previous report (Trzmiel 2017) (Trzmiel 2018). Moreover, in 2015 when an outbreak of BYD on winter wheat and barley was observed, mixed infection of BYDV-MAV or BYDV-PAV and WDV was common but the rate of WDV infection was definitely lower (Trzmiel 2018).…”

Section: Discussionsupporting

confidence: 51%

“…In order to discriminate barley-and wheat-specific forms of wheat dwarf virus (WDV-B and WDV-W, respectively) Duplex-IC-PCR was carried out for 16 winter wheat and 6 winter barley samples. The reactions were performed with WDV-H-F (CAAGGG GCGAGATCACACA) / WDV-H-R (CCACAACTACT ACAACAGCC) and WDV-T-F (CGAGTAGTTGA TGAATGACTCG) / WDV-T-R (GGCTGTTTCAACT CCAGGTCG) primer pairs according to (Trzmiel 2018). Firstly, PCR tubes were coated with 20 µl mixture of 10-fold diluted polyclonal anti-WDV antibody with commercial coating buffer (Loewe).…”

Section: Duplex-immunocapture-polymerase Chain Reaction (Duplex-ic-pcr)mentioning

confidence: 99%

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Journal of Plant Protection Research

Trzmiel¹

2020

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In the spring of 2019, many plants, mainly winter wheat, were observed to have dwarfism and leaf yellowing symptoms. These plants from several regions of Poland were collected and sent to the Plant Disease Clinic of the Institute of Plant Protection -National Research Institute in Poznań to test for the presence of viral diseases. Double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) results showed numerous cases of Wheat dwarf virus (WDV) and a few cases of plant infections caused by Barley yellow dwarf viruses (BYDVs). WDV was detected in 163 out of 236 tested winter wheat plants (69.1%), in 10 out of 27 tested winter barley plants (37%) and in 6 out of 7 triticale plants (85.7%) while BYDVs were found, respectively, in 9.7% (23 out of 236) and in 18.5% (5 out of 27) of tested winter forms of wheat and barley plants. Infected plants came mainly from the regions of Lower Silesia and Greater Poland. Furthermore, individual cases of infections were also confirmed in the following districts: Lubusz, Opole, Silesia, Kuyavia--Pomerania and Warmia-Masuria. Results of Duplex-immunocapture-polymerase chain reaction (Duplex-IC-PCR) indicated the dominance of WDV-W form in wheat and WDV-B form in barley plants. Moreover, results of reverse transcription -polymerase chain reaction (RT-PCR) connected with restriction fragment length polymorphism (RFLP) analysis, performed for 17 BYDVs samples, revealed 8 BYDV-PAS, 4 BYDV-MAV and 2 BYDV-PAV as well as the presence of two mixed infections of BYDV-MAV/-PAS and one case of BYDV-MAV/-PAV. Next, RT-PCR reactions confirmed single BYDV-GAV infection and the common presence of BYDV-SGV. To the best of our knowledge, in 2020 the viruses were not a big threat to cereal crops in Poland.

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Section: Rna Isolationsupporting

confidence: 65%

Section: Discussionsupporting

confidence: 51%

Section: Duplex-immunocapture-polymerase Chain Reaction (Duplex-ic-pcr)mentioning

confidence: 99%

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Journal of Plant Protection Research

Trzmiel¹

2020

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“…WDV population can be clearly divided into barley and wheat forms [ 3 , 6 , 10 , 14 ]. The genomes of these two forms share 84% nucleotide sequence identity however the CP gene is one of the most stable coding regions [ 21 ].…”

Section: Resultsmentioning

confidence: 99%

“…The comparative analysis of this region revealed ca. 83% nucleotide sequence identity between different barley- and wheat- infecting WDV isolates whereas up to 98% within the particular group [ 14 , 21 ]. Taking into account the genetic diversity of the WDV population, the primers for the LAMP assay were designed based on the alignment of CP sequences.…”

Section: Resultsmentioning

confidence: 99%

Development of loop-mediated isothermal amplification assay for rapid detection of genetically different wheat dwarf virus isolates

Trzmiel

Hasiów‐Jaroszewska

2020

Mol Biol Rep

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Wheat dwarf virus (WDV) is considered as one of the most common viruses on cereal crops. Recently, severe outbreaks of WDV have been observed especially on winter wheat in southwestern part of Poland. Moreover, the presence of genetically different WDV-barley-specific and WDV-wheat-specific forms (WDV-B and WDV-W, respectively) was confirmed. In this study, a loop-mediated isothermal amplification assay (LAMP) was developed for the first time for efficient and rapid detection of WDV-B and WDV-W in infected plants. The reaction was performed using a set of three primer pairs: WDVF3/WDVB3, WDVFIB/WDVBIP and WDVLoopF/WDVLoopB specific for coat protein coding sequence. The amplified products were analyzed by direct staining of DNA, gel electrophoresis and real-time monitoring of the amplification curves. The sensitivity of optimized reaction was tenfold higher in comparison with conventional PCR. LAMP assay developed here is a useful and practical method for the rapid detection of different WDV isolates and can be implemented by phytosanitary services.

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