Detection of Amphetamine and Related Amines in Urine by Gas Chromatography and Combined Gas Chromatography-Mass Spectrometry

Kamei, Katsutoshi; Murata, Masayasu; Ishii, Kôsei; Namekata, Masaya; Momose, Atsushi

doi:10.1248/cpb.21.1996

Cited by 15 publications

(5 citation statements)

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“…Quasi molecular ions with an additional p r o t o n were f o u n d in three CI spectra t a k e n u n d e r the same conditions as in the previous report [1] and they showed n u m b e r s of additional d e u t e r i u m on the derivatives. The fragmentation of these derivatives was similar to one of the M A M P derivatives in the E I [9]. H M A M P , and from 0.05 gg to 1.0 tag for A M P and H A M P (Fig.…”

Section: And 2 In Parallel With Those Of H M a M P And Trideuteratedsupporting

confidence: 60%

Simultaneous quantitative analysis of methamphetamine and 4-hydroxymethamphetamine in body fluids by gas chromatography/mass spectrometry

et al. 1988

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Pentodeuterated 4-hydroxymethamphetamine (HMAMP-d5), 1-(4-hydroxyphenyl)-2-(methyl-d3-amino)-propane-1,2-d2 of high quality was prepared and proved to be a most useful internal standard for quantitative analyses of 4-hydroxymethamphetamine (HMAMP), a main metabolite of methamphetamine (MAMP). Highly reliable results were obtained by gas chromatography/mass spectrometry. Simultaneously we determined also MAMP, amphetamine (AMP), 4-hydroxyamphetamine (HAMP), and HMAMP in body fluids, using two internal standards, HMAMP-d5 for HMAMP and HAMP and pentodeuterated methamphetamine, 1-phenyl-2-(methyl-d3-amino)-propane-1,2-d2, for MAMP and AMP.

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Section: And 2 In Parallel With Those Of H M a M P And Trideuteratedsupporting

confidence: 60%

Simultaneous quantitative analysis of methamphetamine and 4-hydroxymethamphetamine in body fluids by gas chromatography/mass spectrometry

et al. 1988

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“…A number of analytical methods for MP and its related compounds have been developed: gas chromatography (GC) (Bruce and Maynard, 1969;Jasin et al, 1974;Terada et al, 1982Terada et al, , 1983Shimosato, 1988;Cox et al, 1Y8Y), gas-chromatography-mass spectrometry (GC-MS) (Morgan et al, 1972;Kamei et al, 1973;Yamamoto et al, 1981 ;Tnoue et al, 1983;Suzuki et al, 1984;Iwasaki et al, 1987), thin layer chromatography (TLC) (Hoof and Heyndrickx, 1974;Miyauchi, 197Y), polarization fluoroimmunoassay (Colbert et al, 1987;Eremin et al, 1987Eremin et al, , 1988, enzyme-or radioimmunoassay (Inayama et al, 1980;Niwaguchi et al, 1982;Aoki and Kuroiwa, 1983) and high performance liquid chromatography (HPLC) (Farrell and Jefferies, Author to whom correspondence should be addressed. 1983; Ishikawa et al, 1984;Takayama et al, 1984;Hayakawa et al, 1989Hayakawa et al, , 1990Nakashima et al, 1990aNakashima et al, , 1991aNakashima et al, , 1991b.…”

Section: Introductionmentioning

confidence: 99%

High performance liquid chromatography with chemiluminescence detection of methamphetamine and its related compounds using 4‐(N,N‐dimethylaminosulphonyl)‐7‐fluoro‐2,1,3‐benzoxadiazole

Nakashima

Suetsugu

Yoshida

et al. 1992

Biomedical Chromatography

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A high performance liquid chromatographic assay of methamphetamine (MP) and its related compounds, i.e. ephedrine (EP), norephedrine (NE), p-hydroxymethamphetamine (p-HMP), p-hydroxyamphetamine (p-HAP) and amphetamine (AP), with peroxyoxalate chemiluminescence detection has been developed. 4-(N,N-Dimethylaminosulphonyl)-7-fluoro-2,1,3-benzoxadiazole (DBD-F) was used as a fluorescent labeling reagent. A mixture of hydrogen peroxide and bis[4-nitro-2-(3,6,9-trioxadecyloxycarbonyl)phenyl] oxalate in acetonitrile was used as a postcolumn chemiluminogenic reagent. DBD derivatives of MP and its related compounds were separated by a gradient elution with acetonitrile and 0.01 M imidazole buffer (pH 7.0) within 65 min. The detection limits (S/N = 3) for the proposed method for MP, AP, EP, NE, p-HMP and p-HAP were 27, 100, 40, 133, 25 and 133 fmol on column, respectively. The recoveries of these compounds with normal urine samples were 87.4-106.4%. The method was successfully applied to the assay of MP and its metabolites in urine samples from MP addicts. A good linear correlation for the resulted amounts of MP or AP between the proposed method and gas chromatography was obtained (r = 0.993 for MP or 0.991 for AP).

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“…The spectra of A-and 3-PPA-TFA have been reported previously [1,[3][4][5][6]. Figure 4 shows the chromatograms of EI-GC/SIM of MA and A extracted from biologic sample with PDM as IS, and Fig.5 that of CI-GC/SIM.…”

Section: Mass Spectra and Gas Chromatograms With Simmentioning

confidence: 63%

“…The ions m/z 158, 120, 119 and 113 on the EI spectrum correspond to m/z 154, 118, and 110 of unlabeled MA-TFA whose fragmentation was described by Kamei [3]. The spectra of A-and 3-PPA-TFA have been reported previously [1,[3][4][5][6].…”

Section: Mass Spectra and Gas Chromatograms With Simmentioning

confidence: 71%

Forensic toxicologic analysis of methamphetamine and amphetamine in body materials by gas chromatography/mass spectrometry

1986

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Qualitative and quantitative analysis of methamphetamine and amphetamine in biologic materials was carried out by gas chromatography/mass spectrometry. A deuterium-labeled methamphetamine was employed as an internal standard with a detection limit of 50 pg and absolute stability and reproducibility. Blood was found to be the best material for estimation of the toxicity of the stimulant drug. It can be replaced by muscle which contains methamphetamine concentrations close to those of blood. The authors' classification of the toxic blood levels of methamphetamine from therapeutic to fatal doses was confirmed by additional data obtained from new case studies.

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Detection of Amphetamine and Related Amines in Urine by Gas Chromatography and Combined Gas Chromatography-Mass Spectrometry

Cited by 15 publications

References 0 publications

Simultaneous quantitative analysis of methamphetamine and 4-hydroxymethamphetamine in body fluids by gas chromatography/mass spectrometry

Simultaneous quantitative analysis of methamphetamine and 4-hydroxymethamphetamine in body fluids by gas chromatography/mass spectrometry

High performance liquid chromatography with chemiluminescence detection of methamphetamine and its related compounds using 4‐(N,N‐dimethylaminosulphonyl)‐7‐fluoro‐2,1,3‐benzoxadiazole

Forensic toxicologic analysis of methamphetamine and amphetamine in body materials by gas chromatography/mass spectrometry

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