Detection of antibacterial activities of Miswak, Kalonji and Aloe vera against oral pathogens & anti-proliferative activity against cancer cell line

Abstract

Background: Emerging drug resistance and hindrance of treatment is provoking scientists to search new, less expensive medicinally active compounds. Dental diseases caused by oral pathogens are very frequent chronic infections around the world. The medical potentials of a lot of Pakistani local herbs and herbal combinations is relatively unknown, hence attempted to explore. A study was designed to investigate potential role of local medicinal herbs for example Miswak, Kalonji & Aloe vera as antimicrobial, antioxidant and anti-proliferative agents against oral pathogens and cancer cell line.Methods: Medicinal extracts were prepared in solvents of different polarities. Their antimicrobial activity was determined alone and in combination against oral pathogens. Antioxidant activity was evaluated through Catalase and Superoxide dismutase assay and anti-proliferative activity was evaluated through 3-(4, 5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide) assay. Results: Plant extracts alone and in combinations were found significantly effective as antimicrobial agent against standard ATCC strains of C. albicans and S. aureus (P ˂0.001). Especially Miwak extract was found highly significant against fungus. Extracts of Kalonji were found significant in inhibiting growth of HeLa cell lines. Miswak and Kalonji showed significant levels of antioxidant activity. Conclusion:Medicinal herbs Miswak and Kalonji have potential to be used for therapeutic purposes. Results suggested that herbal medicinal composition can be prepared using these extracts after applying scientific standardization methods.

Salvadora persica: Nature’s Gift for Periodontal Health

“…The herbal and natural agents frequently used for periodontal treatment include Cinnamomum zeylanicum, Acacia catechu, Propolis, Mikania glomerate, Mikania laevigata, Glycyrrhiza glabra, Droserapeltata, Aloe vera, Allium sativum, Helichrysumitalicum, Piper cubeba, Coptidis rhizome, Azadirachta indica, Nigella Sativa, Syzygium aromaticum and tea tree oil [1,10,31]. Amongst the most promising herbal medications with conspicuous budding future profits for periodontal health and treatment is SP, as a widely used plant in traditional medicine in Africa, the Middle East and Asia for the treatment of a wide range of diseases and conditions [45,46].…”

Antibacterial Properties of Aloe vera on Intracanal Medicaments against Enterococcus faecalis Biofilm at Different Stages of Development

“…Aloe vera is an antibacterial, antiviral, antifungal, and anti-inflammatory herbal compound and is used in toothpaste and mouthwashes. It has also been studied as a canal washing agent [ 13 – 16 ]. This herbal compound has an inhibitory effect on many oral pathogens, including E. faecalis , Streptococcus pyogenes, and Candida albicans , due to the anthraquinone phenolic compounds [ 13 , 17 ].…”

“…Aloe vera is biocompatible and has no toxicity problem in the long term adjacent to the tissues around the root [ 16 , 18 ]. Agar diffusion, broth diffusion, and direct contact method are the most conventional methods to evaluate the antibacterial property of such components [ 13 , 15 , 17 , 19 ]. The purpose of this study was to evaluate the antibacterial properties of Aloe vera gel as an intracanal medicament against E. faecalis biofilm at the 4 th and 6 th week of development by the colony-forming unit (CFU) counting method to see whether Aloe vera gel has equal activity with other conventional antimicrobial agents.…”

Anti-Inflammatory Potential of Aloe Vera in Oral Mucositis Therapy: Systematic Review

“…This causes the scientist to look for steroid replacement alternatives with other drugs with equivalent but more potential, few or no side effects [7]. Currently, herbal products are being widely researched and used with therapeutic purposes because they are considered more economical, relatively safe, and generally have low toxicity [8]; however, the effectiveness and safety of these herbal medicines still need to be scientifically proven before they can be used to prevent or treat disease [9,10].…”