Detection of Antimicrobial Resistance of Bacteria Staphylococcus chromogenes Isolated from Sheep’s Milk and Cheese

Regecová, Ivana; Výrostková, Jana; Zigo, František; Gregová, G.; Kováčová, Mariana

doi:10.3390/antibiotics10050570

Cited by 12 publications

(7 citation statements)

References 85 publications

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“…Significant difference analysis of isolates of Enterococcus sp. antibiotic resistance was made by the odds ratio (OR) test conducted with MedCalc Statistical Software version 19.2.6 (MedCalc Software, Ostend, Belgium) according to Regecová et al [23]. A confidence interval was set to p < 0.05 at 95%.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance of Enterococcus sp. Isolated from Sheep and Goat Cheeses

Výrostková

Regecová

Dudríková

et al. 2021

Foods

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This study aimed to calculate the proportion of antibiotic resistance profiles of Enterococcus faecium, E. faecalis, and E. durans isolated from traditional sheep and goat cheeses obtained from a selected border area of Slovakia with Hungary (region Slanské vrchy). A total of 110 Enterococcus sp. were isolated from cheese samples, of which 52 strains (E. faecium (12), E. faecalis (28), E. durans (12)) were represented. After isolation and identification by polymerase chain reaction and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry, the enterococci (E. faecium, E. faecalis, and E. durans) were submitted to susceptibility tests against nine antimicrobial agents. In general, strains of E. faecalis were more resistant than E. durans and E. faecium. A high percentage of resistance was noted in E. faecalis to rifampicin (100%), vancomycin (85.7%), teicoplanin (71.4%), erythromycin (71.4%), minocycline (57.1%), nitrofurantoin (57.1%), ciprofloxacin (14.3%), and levofloxacin (14.3%). E. durans showed resistance to rifampicin (100%), teicoplanin (100%), vancomycin (66.7%), erythromycin (66.7%), nitrofurantoin (66.7%), and minocycline (33.3%), and E. faecium showed resistance to vancomycin, teicoplanin, and erythromycin (100%). Multidrug-resistant strains were confirmed in 80% of the 52 strains in this study. Continuous identification of Enterococcus sp. and monitoring of their incidence and emerging antibiotic resistance is important in order to prevent a potential risk to public health caused by the contamination of milk and other dairy products, such as cheeses, made on farm level.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance of Enterococcus sp. Isolated from Sheep and Goat Cheeses

Výrostková

Regecová

Dudríková

et al. 2021

Foods

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“…Several other studies have corroborated this situation [95][96][97][98]. Also, antimicrobial resistance of the bacterium Staphylococcus chromogenes was detected in a strain isolated from sheep's milk and cheese [99], showing that dairy products can propagate multi-resistant microorganisms.…”

Section: Foodstuff Contaminationmentioning

confidence: 58%

The Potential of Wood Vinegar to Replace Antimicrobials Used in Animal Husbandry—A Review

Gama,

Pimenta,

Feijó

et al. 2024

Animals

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The indiscriminate use of antimicrobials in animal husbandry can result in various types of environmental contamination. Part of the dose of these products is excreted, still active, in the animals’ feces and urine. These excreta are widely used as organic fertilizers, which results in contamination with antimicrobial molecules. The impacts can occur in several compartments, such as soil, groundwater, and surface watercourses. Also, contamination by antimicrobials fed or administrated to pigs, chickens, and cattle can reach the meat, milk, and other animal products, which calls into question the sustainability of using these products as part of eco-friendly practices. Therefore, a search for alternative natural products is required to replace the conventional antimicrobials currently used in animal husbandry, aiming to mitigate environmental contamination. We thus carried out a review addressing this issue, highlighting wood vinegar (WV), also known as pyroligneous acid, as an alternative antimicrobial with good potential to replace conventional products. In this regard, many studies have demonstrated that WV is a promising product. WV is a nontoxic additive widely employed in the food industry to impart a smoked flavor to foods. Studies have shown that, depending on the WV concentration, good results can be achieved using it as an antimicrobial against pathogenic bacteria and fungi and a valuable growth promoter for poultry and pigs.

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“…Considering the clinical treatment of MDR S. aureus and MRSA strains spread in cheese and other dairy products is challenging, it is imperative to monitor the spread of AMR and resistance mechanisms of this bacterium along its food chains. Practical control measures, including proper pasteurization, HACCP implementation, good hygiene practices (GHP), good manufacturing practices (GMP) from on-farm to off-farm, maintaining time/temperature control for safety (TCS), microbial risk assessment, and performing foodborne illness surveillance, are urgently needed (Choi et al 2016;Liu et al 2017;Regecová et al 2021).…”

Section: Discussionmentioning

confidence: 99%

Prevalence and antimicrobial resistance profiling of Staphylococcus aureus isolated from traditional cheese in Yunnan, China

et al. 2021

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The prevalence of staphylococcal infection and the emergence of multidrug resistance of Staphylococcus aureus (S. aureus) are major concerns in food safety and public health. This study aimed to investigate the prevalence of S. aureus isolated from traditional Chinese Rubing and Rushan cheese, antimicrobial resistance profiles, genomic characteristics, and predict antimicrobial resistance genes (ARGs). From 124 samples, 18 of 62 (29.03%) of Rubing and 5 of 62 (8.06%) of Rushan cheese were confirmed to be S. aureus positive by standard culture-based methods. Twenty-three coagulase-positive staphylococci isolates were grouped into 16 clusters by pulsed-field gel electrophoresis and subjected to routine susceptibility testing to 12 antibiotics. Those isolates exhibited high resistance to penicillin (100%), erythromycin, trimethoprim-sulphamethoxazole (34.78%), oxacillin, clindamycin, and cefoxitin (21.74%). Multidrug-resistant (MDR) S. aureus was found in 34.78% (8 of 23) of isolates. Further, S. aureus strain DC.RB_015 isolated from Rubing cheese, recognized as the most resistant to six antibiotics, was selected for whole-genome sequencing (WGS), continued with in silico approaches. S. aureus DC.RB_015 had a single chromosome size of 2,794,578 bp and a plasmid size of 22,961 bp. The strain harbored 18 predicted ARGs, including eight efflux pump genes (mepA, tet(K), arlR, arlS, norA, mgrA, tet(38), LmrS), one peptidoglycan biosynthesis gene (bacA), two β-lactams resistance genes (mecA, blaZ), and seven genes conferring other antimicrobial resistance (APH(3′)-IIIa, aad(6), ErmB, mecR1, GlpT, murA). The results of this study expand the knowledge of S. aureus strain DC.RB_015, increase food safety awareness, and will be helpful in establishing therapeutic therapy.

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Detection of Antimicrobial Resistance of Bacteria Staphylococcus chromogenes Isolated from Sheep’s Milk and Cheese

Cited by 12 publications

References 85 publications

Antimicrobial Resistance of Enterococcus sp. Isolated from Sheep and Goat Cheeses

Antimicrobial Resistance of Enterococcus sp. Isolated from Sheep and Goat Cheeses

The Potential of Wood Vinegar to Replace Antimicrobials Used in Animal Husbandry—A Review

Prevalence and antimicrobial resistance profiling of Staphylococcus aureus isolated from traditional cheese in Yunnan, China

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