Detection of NDM-1 carbapenemase-producing Acinetobacter calcoaceticus and Acinetobacter junii in environmental samples from livestock farms

Wang, Bing; Sun, Dongchang

doi:10.1093/jac/dku405

Cited by 47 publications

(48 citation statements)

References 13 publications

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“…This bla NDM-1 was harbored by a plasmid also carrying other AMR-genes [ aphA6 , ble and msr (E)- mph (E)] [158]. The bla NDM-1 has been found in other Acinetobacter species [ A. junii (from a pig farm) and A. calcoaceticus (from around a cow farm)] recovered from environmental samples and farm animals [155,166]. A new carbapenemase ( bla OXA-497 ) has been found in A. baumannii from dairy cattle [157].…”

Section: Amr-genes In Gram-negative Bacteria From Animalsmentioning

confidence: 99%

Bacteria from Animals as a Pool of Antimicrobial Resistance Genes

et al. 2017

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Antimicrobial agents are used in both veterinary and human medicine. The intensive use of antimicrobials in animals may promote the fixation of antimicrobial resistance genes in bacteria, which may be zoonotic or capable to transfer these genes to human-adapted pathogens or to human gut microbiota via direct contact, food or the environment. This review summarizes the current knowledge of the use of antimicrobial agents in animal health and explores the role of bacteria from animals as a pool of antimicrobial resistance genes for human bacteria. This review focused in relevant examples within the ESC(K)APE (Enterococcus faecium, Staphylococcus aureus, Clostridium difficile (Klebsiella pneumoniae), Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacteriaceae) group of bacterial pathogens that are the leading cause of nosocomial infections throughout the world.

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Section: Amr-genes In Gram-negative Bacteria From Animalsmentioning

confidence: 99%

Bacteria from Animals as a Pool of Antimicrobial Resistance Genes

et al. 2017

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“…Often by acquiring mobile resistance elements through horizontal gene transfer, CRE infections are especially threatening because they approach pan-resistance, frequently delaying and greatly reducing successful therapeutic treatment options for invasive infections. These bacteria harboring mobile carbapenemase genes are now identified with some regularity from both hospital-and community-acquired human infections (4) and have been recovered from health care environments (5), waste and surface water flows, soil, and companion animals (6,7).…”

mentioning

confidence: 99%

Carbapenemase-Producing Enterobacteriaceae Recovered from the Environment of a Swine Farrow-to-Finish Operation in the United States

Mollenkopf

Stull

Mathys

et al. 2017

Antimicrob Agents Chemother

112

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Carbapenem-resistant Enterobacteriaceae (CRE) present an urgent threat to public health. While use of carbapenem antimicrobials is restricted for foodproducing animals, other ␤-lactams, such as ceftiofur, are used in livestock. This use may provide selection pressure favoring the amplification of carbapenem resistance, but this relationship has not been established. Previously unreported among U.S. livestock, plasmid-mediated CRE have been reported from livestock in Europe and Asia. In this study, environmental and fecal samples were collected from a 1,500-sow, U.S. farrow-to-finish operation during 4 visits over a 5-month period in 2015. Samples were screened using selective media for the presence of CRE, and the resulting carbapenemase-producing isolates were further characterized. Of 30 environmental samples collected from a nursery room on our initial visit, 2 (7%) samples yielded 3 isolates, 2 sequence type 218 (ST 218) Escherichia coli and 1 Proteus mirabilis, carrying the metallo-␤-lactamase gene bla IMP-27 on IncQ1 plasmids. We recovered on our third visit 15 IMP-27-bearing isolates of multiple Enterobacteriaceae species from 11 of 24 (46%) environmental samples from 2 farrowing rooms. These isolates each also carried bla IMP-27 on IncQ1 plasmids. No CRE isolates were recovered from fecal swabs or samples in this study. As is common in U.S. swine production, piglets on this farm receive ceftiofur at birth, with males receiving a second dose at castration (Ϸday 6). This selection pressure may favor the dissemination of bla IMP-27 -bearing Enterobacteriaceae in this farrowing barn. The absence of this selection pressure in the nursery and finisher barns likely resulted in the loss of the ecological niche needed for maintenance of this carbapenem resistance gene.

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“…Horizontal gene transfer of antibiotic resistance (AMR) genes occurs via the processes of transformation and conjugation. The former mediates especially narrow range, intra-genus transfers 1,2 , whereas the latter is implicated in a wider range of transfer hosts 3,4 and potentially enables AMR to overcome the toughest phylogenetic and ecological transmission barriers [5][6][7][8][9][10] .…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, the interaction between conjugative relaxase enzymes and their DNA origin-of-transfer (oriT) substrates facilitates the majority of all AMR transfers in nature 11,12 and is especially important for ones related to human infection complications 13 . However, the current knowledge on conjugative transfer mechanisms and systems 4,[14][15][16] is unable to describe the unprecedented amount of observed horizontal transfer 7,8,12,17 that seems to transcend all transfer barriers between resistance reservoirs and human hosts [5][6][7]9,10,12,13,18 .…”

Section: Introductionmentioning

confidence: 99%

Multiple plasmid origin-of-transfer substrates enable the spread of natural antimicrobial resistance to human pathogens

Zrimec

2020

Preprint

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Antimicrobial resistance poses a great danger to humanity, in part due to the widespread horizontal transfer of plasmids via conjugation. Modeling of plasmid transfer is essential to uncovering the fundamentals of resistance transfer and for development of predictive measures to limit the spread of resistance. However, a major limitation in the current understanding of plasmids is the inadequate characterization of the DNA transfer mechanisms, which conceals the actual potential for plasmid transfer in nature. Here, we consider that the plasmid-borne origin-of-transfer substrates encode specific DNA structural properties that can facilitate finding these regions in large datasets, and develop a DNA structure-based alignment procedure for typing the transfer substrates. Since our method outperforms current sequence-based approaches, we identify thousands of yet undiscovered DNA transfer substrates, showing that plasmid mobility is in fact 2-fold higher and spans almost 2-fold more host species than is currently known. Over half of all mobile plasmids contain the means to transfer between different mobility groups, which links previously confined host ranges across ecological habitats into a vast network of potential plasmid transfers. Certain types of conjugative transfer mechanisms and their corresponding hosts are transfer hubs that help break down the horizontal gene transfer barriers and form a robust gene flow system. We show that this network in fact serves to transfer resistance from the environmental genetic reservoirs to human MDR pathogens, which is an important driver of the observed rapid resistance development in humans and thus an important point of focus for future prevention measures.

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Detection of NDM-1 carbapenemase-producing Acinetobacter calcoaceticus and Acinetobacter junii in environmental samples from livestock farms

Cited by 47 publications

References 13 publications

Bacteria from Animals as a Pool of Antimicrobial Resistance Genes

Bacteria from Animals as a Pool of Antimicrobial Resistance Genes

Carbapenemase-Producing Enterobacteriaceae Recovered from the Environment of a Swine Farrow-to-Finish Operation in the United States

Multiple plasmid origin-of-transfer substrates enable the spread of natural antimicrobial resistance to human pathogens

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