Detection of Rotaviruses by Nucleic Acid Hybridization with Cloned DNA of Simian Rotavirus SA11 Genes

Dimitrov, D; Graham, D.Y.; Estes, Mary K.

doi:10.1093/infdis/152.2.293

Cited by 53 publications

(36 citation statements)

References 15 publications

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“…Various methods to detect rotavirus or rotavii-al proteins in infected cell cultures have been developed and include a collodial gold-protein A-IgG technique (289), a radioimmunofocus assay (220), and an immunoperoxidase procedure (44). More recently, several sensitive and specific research techniques for the detection of rotavirus in clinical specimens have been developed, which include a dot-immunobinding assay (299), a dotavidin-biotin-amplified immunobinding assay (299), an avidin-biotin RIA (407), and several dot hybridization techniques (82,100,116,217 (155,159) found that the immunoglobulin secretory component (ScIg) increased in serum 1 to 2 weeks after disease onset; however, it had disappeared by 4 months. Grauballe et al (129) detected sIgA in patient sera for only 4 to 10 days after rotavirus was detected in stools, and this correlated well with recent infection.…”

Section: Epidemiologymentioning

confidence: 99%

Human viral gastroenteritis

Christensen

1989

Clin Microbiol Rev

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During the last 15 years, several different groups of fastidious viruses that are responsible for a large proportion of acute viral gastroenteritis cases have been discovered by the electron microscopic examination of stool specimens. This disease is one of the most prevalent and serious clinical syndromes seen around the world, especially in children. Rotaviruses, in the family Reoviridae, and fastidious fecal adenoviruses account for much of the viral gastroenteritis in infants and young children, whereas the small caliciviruses and unclassified astroviruses, and possibly enteric coronaviruses, are responsible for significantly fewer cases overall. In addition to electron microscopy, enzyme immunoassays and other rapid antigen detection systems have been developed to detect rotaviruses and fastidious fecal adenoviruses in the stool specimens of both nonhospitalized patients and those hospitalized for dehydration and electrolyte imbalance. Experimental rotavirus vaccines have also been developed, due to the prevalence and seriousness of rotavirus infection. The small, unclassified Norwalk virus and morphologically similar viruses are responsible for large and small outbreaks of acute gastroenteritis in older children, adolescents, and adults. Hospitalization of older patients infected with these viruses is usually not required, and their laboratory diagnoses have been limited primarily to research laboratories.

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Section: Epidemiologymentioning

confidence: 99%

Human viral gastroenteritis

Christensen

1989

Clin Microbiol Rev

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“…Probes to the herpes viruses (2,3,13,16,20,54,74,88,103,115,122,125,126,143), adenoviruses (71,90,142), enteroviruses (58,108,109), reoviruses (26), hepatitis viruses (6,8,9,67,114), papillomaviruses (5,96), papovaviruses (44), and parvoviruses (22) have all been described.…”

Section: Definitionsmentioning

confidence: 99%

Diagnostic deoxyribonucleic acid probes for infectious diseases

Tenover

1988

Clin Microbiol Rev

239

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Virtually all microorganisms contain some unique nucleotide sequences which can be the target of deoxyribonucleic acid probes. Probes have been used successfully to identify a wide variety of pathogens from the simple ribonucleic acid-containing polioviruses to the complex filarial worms Brugia malayi. Probe technology offers the clinical laboratory the potential both to extend the types of pathogens that can be readily identified and to reduce significantly the time associated with the identification of fastidious microorganisms. Over a dozen commercially prepared deoxyribonucleic acid probe tests are now available. This article explores the development of deoxyribonucleic acid probe tests and reviews the sensitivity, specificity, and predictive values of many of the diagnostic probes developed during the last several years. Prospects for newer, more sensitive detection systems for the products of hybridization reactions are also reviewed.

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“…The cDNA probe was able to detect 50 ng of TRV dsRNA. The sensitivity reported for rotavirus probes in this type of assays is in the range of 0.5 to 5 ng of RNA (Dimitrov et al 1985, Eiden & Yolken 1987. We are attempting to improve the sensitivity of the cDNA probe by increasing the specific activity of the cDNA and by using highly specific RNA probes as described by Kreig & Melton (1984).…”

Section: Discussionmentioning

confidence: 89%

A genetic probe for identification of the turbot aquareovirus in infected cell cultures

Lupiani¹,

Subramanian²,

Hetrick³

et al. 1993

Dis. Aquat. Org.

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A nucleic acid hybridization assay has been developed that can detect small amounts of the turbot aquareovirus (TRV) in infected cells. Complementary DNAs were synthesized from the dsRNA genome segments of TRV and used to generate a large number of recombinant plasmids. Plasrnids corresponding to genomic segments 1 through 8 were identified by Northern blot analysis. A randomly selected clone, Clone 66, hybridizing to genome Segment 8, showed high specificity hybridizing only RNA from TRV, and not with the RNAs of 4 other aquareoviruses, or with RNA extracted from infectious pancreatic necrosis virus (IPNV), infectious hematopoietic necrosis virus (IHNV) or with uninfected cells. A 32~-labeled probe was able to detect as little as 50 ng of TRV purified virus dsRNA. Time course experiments indicated that TRV RNA can be detected in infected cells 96 h post-inoculation, a time when cytopathic effect is still not evident. These results indicate that the dot blot assay described here could be used as an effective diagnostic tool for the detection of TRV infections.

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Detection of Rotaviruses by Nucleic Acid Hybridization with Cloned DNA of Simian Rotavirus SA11 Genes

Cited by 53 publications

References 15 publications

Human viral gastroenteritis

Human viral gastroenteritis

Diagnostic deoxyribonucleic acid probes for infectious diseases

A genetic probe for identification of the turbot aquareovirus in infected cell cultures

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