Detection of – rs53576 (C→T) polymorphism of OXTR gene in patients with DMT2 in Mosul city

Abstract

Background, Oxytocin it is a neuro-protein hormone, which is responsible for many process and behaviour in the body, study indicates that this hormone considered anti diabetic and anti-obesity, and play vital role in regulation of blood sugar, insulin secretion and metabolism of carbohydrate. The activation of Oxytocin hormone correlated with receptor of oxytocin in the brain.This research aims to discover the association of OXTR (rs53576) gene polymorphismwith risk factor of DMT2.Methods, This study include 28 patients with DMT2 with 20 persons as control groups. The blood collected from each subject and divided in two types, one for seru m that used to determine some biochemical test and another EDTA tube for DNA extraction the used for molecular test. And measured the R.B.S , Ure a, Creatinine and Uric acid by Reflatrone technique, and the DNA extraction then the concentration and purity measured by Biodrop technique, and the determination polymorphism of OXTR gene (rs53576) done by ARMS-PCR technique.Results, The result showed the distribution of allele and genotype for OXTR gene polymorphism in location rs53576 (C→T) different significantly between patients and control group, and also present all genotype for OXTR gene polymorphism in patients group, And the mutant allele frequency is high in patients comparing with healthy persons, On another side the every genotype and allele frequency for OXTR gene polymorphism different between patient and healthy people. Conclusion:However, this study showed the mutant genotype TT and mutant allele T of OXTR gene in position rs53576 have significant difference between groups stud y.