Babies with any type of metabolic disorders lack the ability to break down the food well, which may induce too little amino acids, phenylalanine and blood sugar to the body, there are numerous kinds of this disorders, most of babies with a genetic metabolic disease have many mutation in gene that coded an enzyme which results a deficiency in same enzyme are hundreds of these disorders and they were diagnosed by their symptoms and the treatment method. The treatment methods of the metabolic disorder depend on the specific type of disorders, inborn metabolic disease are some-time  treated with dietary guidance, and other childcare choices, many hereditary metabolic disease are initially caused by gene mutations and that transferred from parents to offspring.

Environmental pollution Pollution is a growing undertaking for humans around the globe which affects in which affects both developed and growing towns (Suresh and Ravishankar, 2004). All herbal methods that make contributions to economic pollution are typically categorized as natural contaminants (Sosa Alderete et al., 2009). These are the human final products which include: navy explosives, agriculture, oil products, gasoline manufacturing and wood results (Pilon-Smits, 2005). Subsequently, the natural activities can additionally sell the discharge of heavy metals as nickel, molybdenum, lead, copper, zinc and mercury to the environment which are harmful to human health (Nedelkoska and Doran, 2000). The whole surroundings are constantly being contaminated from pollutants which is unsafe for human fitness (Suza et al., 2008 ; Rezek et al., 2012). The cost of cleansing up infected sites is high therefore using vegetation to extract, stabilize and degrade contaminants, all of them called as phytoremediation, is giving reputation as an extra costpowerful opportunity to different strategies of cleanup (Kuiper et al., 2004).

TP53 gene plays a critical role in the follow-up of different cancer cases, including diagnosis and follow-up treatment and the mutation in the P53 gene. It harms the encoded P53 protein and the less function of P53 protein in different types of cancer due to the mutation in the TP53 gene. P53 protein has many mechanisms to eliminate cancer cells, like apoptosis, cell cycle arrest, and DNA repair. This study aims to detect the abnormality change in the sequence of P53 mutation and the correlation with cancer in Mosul city DNA extraction depends on the manual description from blood, the Pro72Arg in exon 4 G\C allele mutation measurement by ARMS-PCR, and the analysis of common mutation in Exon 3,4,5 by DNA sequencing technique. The result of this study shows the observation of different genotypes and allelic frequencies of Pro72Arg polymorphism in exon 4. It was the presence of wild-type genotype CC (pro\pro) 12%, hetero genotype CG (pro\arg) 72% and mutant genotype GG (arg\arg) 16% in patients with Lung cancer. While in healthy people, the wild genotype CC was 26 hetero genotype CG 66 and mutant genotype GG 8%. As for the result of DNA sequencing, this research doesn't find any change in the nucleotide of Exon 5 for the P53 gene of the case study. At the same time, the DNA sequence result of Exon 6 for the P53 gene in patients finds some changes in the nucleotide sequence with sequence. According to this study, the observation of different genotypes and allelic frequency of Pro72Arg polymorphism in exon 4 for the P53 gene present significant variation between patients with lung cancer and healthy group male with Lung cancer Keywords: P53 gene, ARMS-PCR, Mutation, SNP, Lung cancer, Exon.

The VDBP amino acid sequence is divided into three domains and has 458 amino acids. Exon 11 in domain III has two SNPs, rs7041 and rs4588, each corresponding to one of the major VDBP kinds (VDBP1S, VDBP1F, and VDBP2). Transport of vitamin D3 to the liver and 25(O.H.)D to the kidney, 1,25(O.H.)2D transport to target organs is mediated by vitamin D-binding protein, which interacts with a particular vitamin D receptor (VDR) to explain biological activities. This study aims to determine whether the VDBP gene has three polymorphisms (rs17467825, rs7041, and rs4588) associated with women with osteoporosis in Mosul city. This study included (96) women, the age range between (35-55) years.The samples were divided into two groups, the first group included (74) women with osteoporosis, and the second group was considered a control group. In this study, DNA was extracted from the blood of all the samples included in the study (95) samples, using the method modified by the researchers. And detection of three different SNP of VDBP, (rs17467825) Polymorphism by ARMS-PCR, and (rs7041T) Polymorphism with (rs4588C) Polymorphism by RFLP-PCR. This study showed a connection between deficiency of vitamin D in women and mutation of the VDBP gene in location rs17467825.

Background, Oxytocin it is a neuro-protein hormone, which is responsible for many process and behaviour in the body, study indicates that this hormone considered anti diabetic and anti-obesity, and play vital role in regulation of blood sugar, insulin secretion and metabolism of carbohydrate. The activation of Oxytocin hormone correlated with receptor of oxytocin in the brain.This research aims to discover the association of OXTR (rs53576) gene polymorphismwith risk factor of DMT2.Methods, This study include 28 patients with DMT2 with 20 persons as control groups. The blood collected from each subject and divided in two types, one for seru m that used to determine some biochemical test and another EDTA tube for DNA extraction the used for molecular test. And measured the R.B.S , Ure a, Creatinine and Uric acid by Reflatrone technique, and the DNA extraction then the concentration and purity measured by Biodrop technique, and the determination polymorphism of OXTR gene (rs53576) done by ARMS-PCR technique.Results, The result showed the distribution of allele and genotype for OXTR gene polymorphism in location rs53576 (C→T) different significantly between patients and control group, and also present all genotype for OXTR gene polymorphism in patients group, And the mutant allele frequency is high in patients comparing with healthy persons, On another side the every genotype and allele frequency for OXTR gene polymorphism different between patient and healthy people. Conclusion:However, this study showed the mutant genotype TT and mutant allele T of OXTR gene in position rs53576 have significant difference between groups stud y.