Determination of Beryllium and Selenium in Human Urine and of Selenium in Human Serum by Graphite-Furnace Atomic Absorption Spectrophotometry

Wang, Hou-Chuan; Peng, Hsien-Wen; Kuo, Mao-Sung

doi:10.2116/analsci.17.527

Cited by 33 publications

(10 citation statements)

References 29 publications

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“…Despite the complexity of serum and blood matrices, no signal suppression was noticed following our optimal conditions and our beryllium detection limits (2 to 7 ng L -1 ) using the Varian AA280Z Zeeman atomic absorption spectrometer are among the lowest reported to date. [ 40 ] and [ 26 ], reported a detection limit of 370 ng L -1 and 4.3 ng L -1 respectively for beryllium in urine by graphite furnace atomic absorption spectrometry. In a similar work, [ 27 ], and [ 24 ], reported a detection limit of 50 ng L -1 for beryllium in urine.…”

Section: Discussionmentioning

confidence: 99%

Graphite furnace atomic absorption spectrometry as a routine method for the quantification of beryllium in blood and serum

Stephan

Fournier

Brousseau

et al. 2008

Chemistry Central Journal

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Background: A routine method for the quantification of beryllium in biological fluids is essential for the development of a chelation therapy for Chronic Beryllium Disease (CBD). We describe a procedure for the direct determination of beryllium in undigested micro quantities of human blood and serum using graphite furnace atomic absorption spectrometry. Blood and serum samples are prepared respectively by a simple 8-fold and 5-fold dilution with a Nash Reagent. Three experimental setups are compared: using no modifier, using magnesium nitrate and using palladium/ citric acid as chemical modifiers.

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Section: Discussionmentioning

confidence: 99%

Graphite furnace atomic absorption spectrometry as a routine method for the quantification of beryllium in blood and serum

Stephan

Fournier

Brousseau

et al. 2008

Chemistry Central Journal

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“…Another disadvantage of using ICP-MS to de-tect and quantify liver Se is the potential for occurrences of interferences through mass overlap. These can include formation of doubly charged ions ( 78 Ge ϩϩ interferes with 78 Se), interferences from elements with the same isotopic mass ( 74 Ge will interfere with 74 Se), and formation of polyatomic ions derived from argonbased plasma, especially for elements with m/z less then 80. Selenium detection can suffer from polyatomic interference from an argon dimer ( 40 Ar 40 Ar ϩ ) that has the same mass as the most abundant Se isotope ( 80 Se ϭ 49.6%).…”

Section: Discussionmentioning

confidence: 99%

“…40 In brief, these include the need for a large sample size for HG-AAS and FL, the requirement for destruction of organic matter before analysis with use of concentrated acids for GC-ECD, FL, HG-AAS, and ICP-AES, 2,5 the need for sample derivatization for GC-ECD and FL, 57,63 the need for oxidizing conditions to prevent volatilization of Se in ETAAS, 48 and the need to use a matrix modifier for ETAAS and GFAAS. 14,41,42,74 Available reports have shown that HG-AAS is 1 of the most widely used methods for Se detection in biological samples. This method allows minimum matrix interferences because of selective separation of Se from the matrix by generation of volatile covalent hydrides, although all the Se has to be converted to a form that will result in hydride formation.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Selenium Determination in Liver Samples by Atomic Absorption Spectroscopy and Inductively Coupled Plasma–Mass Spectrometry

et al. 2005

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Abstract. Selenium (Se) is an essential trace element that is often deficient in the natural diets of domestic animal species. The measurement of Se in whole blood or liver is the most accurate way to assess Se status for diagnostic purposes. This study was conducted to compare hydride generation atomic absorption spectroscopy (HG-AAS) with inductively coupled plasma-mass spectrometry (ICP-MS) for the detection and quantification of Se in liver samples. Sample digestion was accomplished with magnesium nitrate and nitric acid for HG-AAS and ICP-MS, respectively. The ICP-MS detection was optimized for 82 Se with yttrium used as the internal standard and resulted in a method detection limit of 0.12 g/g. Selenium was quantified by both methods in 310 samples from a variety of species that were submitted to the Toxicology Laboratory at New Bolton Center (Kennett Square, PA) for routine diagnostic testing. Paired measurements for each sample were evaluated by a mean difference plot method. Limits of agreement were used to describe the maximum differences likely to occur between the 2 methods. Results suggest that under the specified conditions ICP-MS can be reliably used in place of AAS for quantitation of tissue Se at or below 2 g/g to differentiate between adequate and deficient liver Se concentrations.

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“…Over recent years numerous analytical techniques have been employed to detect Be(II) in different samples. These include spectrophotometric methods [4][5][6][7], spectrofluorimetric methods [8,9], flame atomic absorption spectroscopy (FAAS) [10], electrothermal atomic absorption spectroscopy (ET-AAS) [11][12][13], inductively coupled plasma atomic emission spectroscopy (ICP-AES) [14] and several other techniques [15][16][17][18]. In contrast to the majority of these *Corresponding author.…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometric determination of trace amounts of beryllium using 1,8-dihydroxyanthrone as a new chromogenic reagent

Beiraghi¹,

Babaee²

2007

JICS

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A simple, rapid, and sensitive spectrophotometric method for the trace level determination of beryllium based on the formation of a 1:2 complex with anthralin (1,8-dihydroxyanthrone) as a new reagent is developed. A spectrophotometric method was used to determine the acidity constant and stepwise proton dissociation of the reagent. The experimental conditions for determining beryllium including the influences of pH, reagent concentration and time were evaluated and optimized. Under the optimum experimental conditions, the molar absorptivity of the complex is 0.47 × 10 4 l mol -1 cm -1 at 545 nm. Calibration graph was linear in the range of 0.04-1.04 μg ml -1 with a detection limit of 0.012 μg ml -1 and a %RSD of 0.43%, for 5 replicate determinations at 0.48 μg ml -1 of Be( ). The interferring effect of some cations and anions was also studied. The method was applied for the determination of beryllium in beryl, silicate rock and alloys. Ethylenediaminetetraacetic acid (EDTA) was used for masking interfering ions.

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Determination of Beryllium and Selenium in Human Urine and of Selenium in Human Serum by Graphite-Furnace Atomic Absorption Spectrophotometry

Cited by 33 publications

References 29 publications

Graphite furnace atomic absorption spectrometry as a routine method for the quantification of beryllium in blood and serum

Graphite furnace atomic absorption spectrometry as a routine method for the quantification of beryllium in blood and serum

Comparison of Selenium Determination in Liver Samples by Atomic Absorption Spectroscopy and Inductively Coupled Plasma–Mass Spectrometry

Spectrophotometric determination of trace amounts of beryllium using 1,8-dihydroxyanthrone as a new chromogenic reagent

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