Determination of Carotene in Dehydrated Alfalfa. A Simplified Method

Silker, Ralph E.; Schrenk, W. G.; King, H. H.

doi:10.1021/i560132a012

Cited by 20 publications

(13 citation statements)

References 14 publications

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“…PRELIMINARY OBSERVATIONS ' Most plant materials, when fresh, contain about 80% of moisture; hence, any method of direct extraction of carotene should be preceded by drying, for which purpose preliminary blanching, and use of alcohol, acetone, and water-binding agents like anhydrous sodium sulfate, sodium carbonate, and calcium sulfate have been recommended (3,8,17,26,41,42,48). As freshly ignited lime is a good desiccant as well as adsorbent for chlorophyll and xanthophylls, a number of experiments were carried out in which known amounts of leaf were macerated in a mortar and further triturated with varying proportions of freshly prepared lime, chemically pure and ignited for a few minutes at a temperature of 250°to 300°C.…”

Section: Methodsmentioning

confidence: 99%

Determination of Carotene in Plant Materials

Sreenivasan¹,

Vaidya²

1948

Anal. Chem.

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A rapid method for the routine determination of carotene in plant materials has been worked out by which the green tissues are triturated with a mixture of lime and anhydrous sodium sulfate. This results in their disintegration as well as adsorption of chlorophyll and other noncarotenoid pigments; the carotene itself is rendered readily extractable by petroleum ether or benzene and can be determined either as gross carotene or as its isomerides after chromatographic resolution. The method is particularly suitable for the analysis of green, leafy materials, feeds, and fodders and gives excellent agreement with standard procedures, but it can be applied with equal success to fresh fruits and vegetables.

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Section: Methodsmentioning

confidence: 99%

Determination of Carotene in Plant Materials

Sreenivasan¹,

Vaidya²

1948

Anal. Chem.

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“…Carotene was determined by the method of Silker, Schrenk, and King (8), amino acid nitrogen by the technique of Beauchene and others (2), and nonprotein nitrogen by the method of Loomis and Shull (5). Fluorescence measurements of aqueous extracts were made as described for soybeans by Milner and Thompson (7).…”

Section: Graded Series Representing Progressive Heating Deteriorationmentioning

confidence: 99%

Crop Storage, Feedstuffs Values and Chemical Changes in Spontaneously Heated Alfalfa Pellets

Ruliffson¹,

Milner²,

Mitchell³

1956

J. Agric. Food Chem.

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solution was, in turn, poured into the spectrometer sample holder and Z)max determined several times over a period of 24 hours. During this period both nance results change by only 4.7% when the moisture content changes by 14.5%; consequently the percentage change in -Dmax, the value measured in the nuclear magnetic resonance spectrometer, is only about one third as much as the percentage change in water content.

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“…The meals were stored in 4-ounce bottles at 25°C. and were analyzed for carotene at intervals by the method of Silker, Schrenk, and King (6). The aniline, monomethylaniline, and dimethylaniline used in the experiment w'ere pur-chased from various chemical supply houses.…”

Section: Aniline Derivatives As Antioxidantsmentioning

confidence: 99%

“…Table II shows the per cent of carotene which was lost in the heated and unheated portions during 6 months of storage at 25°C.…”

Section: Effects Of Heat and Oil Levelmentioning

confidence: 99%