Determination of composition and structure of spongy bone tissue in human head of femur by Raman spectral mapping

Kozielski, M.; Buchwald, Tomasz; Szybowicz, Mirosław; Błaszczak, Zdzisław; Piotrowski, A; Ciesielczyk, Błazej

doi:10.1007/s10856-011-4353-0

Cited by 64 publications

(60 citation statements)

References 30 publications

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“…250 nm). Despite that, advances in instrumentation have enabled high-resolution, position-resolved analyses of bone ultrastructure orientation [81,82]. This is often combined with composition analysis [90,91], which is an inherent capability of Raman spectroscopes to provide properties that determine different bone quality [92] and other clinically relevant [88,93] properties.…”

Section: Polarized Raman Spectroscopymentioning

confidence: 99%

“…The high brilliance of synchrotron radiation (SR) facilities and recent advances in fast-readout and low-noise detectors have enabled fast acquisition of X-ray scattering patterns, [67] with kind permission of PLoS, peak assignments according to [81].) (b) A composite of 2D images based on polarized Raman spectral analysis, resulting in a 3D representation of two orthogonal planes of an osteonal structure of human cortical bone.…”

Section: X-ray-based Techniques 2221 Small-angle/wide-angle X-raymentioning

confidence: 99%

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Techniques to assess bone ultrastructure organization: orientation and arrangement of mineralized collagen fibrils

Georgiadis

Müller

Schneider

2016

J. R. Soc. Interface.

113

100

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Bone's remarkable mechanical properties are a result of its hierarchical structure. The mineralized collagen fibrils, made up of collagen fibrils and crystal platelets, are bone's building blocks at an ultrastructural level. The organization of bone's ultrastructure with respect to the orientation and arrangement of mineralized collagen fibrils has been the matter of numerous studies based on a variety of imaging techniques in the past decades. These techniques either exploit physical principles, such as polarization, diffraction or scattering to examine bone ultrastructure orientation and arrangement, or directly image the fibrils at the sub-micrometre scale. They make use of diverse probes such as visible light, X-rays and electrons at different scales, from centimetres down to nanometres. They allow imaging of bone sections or surfaces in two dimensions or investigating bone tissue truly in three dimensions, in vivo or ex vivo, and sometimes in combination with in situ mechanical experiments. The purpose of this review is to summarize and discuss this broad range of imaging techniques and the different modalities of their use, in order to discuss their advantages and limitations for the assessment of bone ultrastructure organization with respect to the orientation and arrangement of mineralized collagen fibrils.

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Section: Polarized Raman Spectroscopymentioning

confidence: 99%

Section: X-ray-based Techniques 2221 Small-angle/wide-angle X-raymentioning

confidence: 99%

Techniques to assess bone ultrastructure organization: orientation and arrangement of mineralized collagen fibrils

Georgiadis

Müller

Schneider

2016

J. R. Soc. Interface.

113

100

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“…29 The ingredients of Gel and BaG composite mimic the natural organic and mineral constituents of bone, which are collagen fibers and hydroxyapatite crystals. 30 In a recent investigation, the optimized composition of BaG and Gel for bone tissue scaffolds was reported to be 30:10 (weight percent [wt%] in the stock solution). 31 In this study, 0.1%-0.3% of PEDOT:PSS was added to this optimized value.…”

Section: Introductionmentioning

confidence: 99%

3D conductive nanocomposite scaffold for bone tissue engineering

Shahini¹,

Yazdimamaghani²,

Walker³

et al. 2013

IJN

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Bone healing can be significantly expedited by applying electrical stimuli in the injured region. Therefore, a three-dimensional (3D) ceramic conductive tissue engineering scaffold for large bone defects that can locally deliver the electrical stimuli is highly desired. In the present study, 3D conductive scaffolds were prepared by employing a biocompatible conductive polymer, ie, poly(3,4-ethylenedioxythiophene) poly(4-styrene sulfonate) (PEDOT:PSS), in the optimized nanocomposite of gelatin and bioactive glass. For in vitro analysis, adult human mesenchymal stem cells were seeded in the scaffolds. Material characterizations using hydrogen-1 nuclear magnetic resonance, in vitro degradation, as well as thermal and mechanical analysis showed that incorporation of PEDOT:PSS increased the physiochemical stability of the composite, resulting in improved mechanical properties and biodegradation resistance. The outcomes indicate that PEDOT:PSS and polypeptide chains have close interaction, most likely by forming salt bridges between arginine side chains and sulfonate groups. The morphology of the scaffolds and cultured human mesenchymal stem cells were observed and analyzed via scanning electron microscope, micro-computed tomography, and confocal fluorescent microscope. Increasing the concentration of the conductive polymer in the scaffold enhanced the cell viability, indicating the improved microstructure of the scaffolds or boosted electrical signaling among cells. These results show that these conductive scaffolds are not only structurally more favorable for bone tissue engineering, but also can be a step forward in combining the tissue engineering techniques with the method of enhancing the bone healing by electrical stimuli.

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“…In Raman spectra, the main vibrational bands related to the wound healing process are those corresponding to the presence of collagen-forming protein (Kozielski et al, 2011;Lieber et al, 2008). Collagen spectral bands are found at the following positions: 1,730-1,740 collagen III; 1,654-1,655 amide I (C=O stretching mode of proteins, α-helix conformation)/C=C lipid stretch; 1,450-1,500 stretching (CH 2 )-lipids, glycosaminoglycans, metalloproteinases, collagens and residues, and 1,245-1,345 amide IIIcollagen.…”

Section: Discussionmentioning

confidence: 99%

“…Studies using this technique are performed to identify the tissue and differentiate Volume 30, Número 1, p. 47-53, 2014 between normal and pathological spectra, according to the structure and chemical composition of the tissue (Kozielski et al, 2011). The spectra are classified according to the main biochemical components of the tissue using statistical methods such as principal component analysis and linear discriminant analysis (Ebaid et al, 2011;Kozielski et al, 2011;Lieber et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

FT-Raman spectroscopic study of skin wound healing in diabetic rats treated with Cenostigma macrophyllum Tul

Coelho

Raniero

Costa

et al. 2014

RBEB

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Introduction: Patients with diabetes mellitus exhibit a delay in the lesion repair process. The active components of Cenostigma macrophyllum may represent a viable alternative to facilitate the recovery of these lesions. The aim of this study was to evaluate the effects of emulsion oil-water Cenostigma macrophyllum in the repair process of lesions in rats with induced diabetes. Methods: 63 male rats (Wistar, 200-250 g body weight, 30-40 days old) were distributed into the following groups: control (C), diabetic (D) and diabetic treated with Cenostigma macrophyllum (P), subdivided based on the experimental times, days 7, 14 and 28, with 21 animals per main group. Diabetes mellitus (DM) was induced by administration of streptozotocin (50 mg/kg via penile vein and 12-h fasting) and confirmed at day 21 (glycemic index > 240 mg/dL). In the animals of group P, 0.5 ml of the oil-water emulsion obtained from the plant seed was used. The samples were removed and hemisectioned, and one portion was used for the quantitative histological analysis of collagen using Masson's trichrome staining, while another portion was analyzed by FT-Raman spectroscopy. Results: A higher percentage area of the volume of collagen fibers was observed for the experimental time Day 14 in group P compared with group D (p < 0.001). Regarding the ratio of areas of the amides I (1700-1600 cm -1 ) and III (1245-1345 cm -1 ), the groups D and P show the opposite behavior. Conclusion: Cenostigma macrophyllum accelerated the repair process in skin of diabetic ratsfor14 days.

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Determination of composition and structure of spongy bone tissue in human head of femur by Raman spectral mapping

Cited by 64 publications

References 30 publications

Techniques to assess bone ultrastructure organization: orientation and arrangement of mineralized collagen fibrils

Techniques to assess bone ultrastructure organization: orientation and arrangement of mineralized collagen fibrils

3D conductive nanocomposite scaffold for bone tissue engineering

FT-Raman spectroscopic study of skin wound healing in diabetic rats treated with Cenostigma macrophyllum Tul

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