Determination of ecdysteroids in Fornicium unflorum (L.) and Serratula centauroides (L.) raw materials by chromatography–UV spectrophotometry

Николаева, И. Г.; Tsybiktarova, L. P.; Garmaeva, L. L.; Николаева, Г. Г.; Оленников, Д. Н.; Matkhanov, I. E.

doi:10.1134/s1061934817080093

Cited by 14 publications

(9 citation statements)

References 8 publications

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“…Three basic compounds of R. uniflorum extracts were 5-O-caffeoylquinic acid, 4-O-caffeoylquinic acid and 20-hydroxyecdysone with the level of content at 63.59, 25.11 and 17.83 mg/g, respectively, as well in S. centauroides extract the major compounds were 5-O-caffeoylquinic acid (40.85 mg/g), 20-hydroxyecdysone (27.43 mg/g) and apigenin-7-O-glucuronide (18.27 mg/g) (Table 1). The remaining identified compounds were mainly di-O-caffeoylquinic acids and O-glycosylflavones found previously in herb of R. uniflorum [15,16,18,19].…”

Section: Hplc-dad-esi-ms Profiles Of Rhaponticum Uniflorum and Serratula Centauroides Extractsmentioning

confidence: 63%

“…Ecdysteroids in the plant were first revealed in the early 1990s and by now its ecdysteroid profile includes more than 30 individual compounds, among them, 20-hydroxyecdysone and ajugasterone derivatives are common. Some other bioactive substances have been found in R. uniflorum as flavonoids, di-and triterpenoids, sesquiterpenes, organic acids, lignans, essential oils, polysaccharides, amino acids, vitamins and fatty acids [14][15][16]. The pharmacological activity of R. uniflorum was shown mainly for the root extracts, decoction and solvent fractions.…”

Section: Introductionmentioning

confidence: 99%

“…The S. centauroides herb contains ecdysteroids with the highest concentration in the leaves (20.64 mg/kg) and stems (16.20 mg/kg). 20-Hydroxyecdysone is the basic compound in all organs [15,18]. Among other compounds, this plant also contains phenolic compounds, amino acids, fatty and organic acids and essential oils.…”

Section: Introductionmentioning

confidence: 99%

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Rhaponticum uniflorum and Serratula centauroides Extracts Attenuate Emotional Injury in Acute and Chronic Emotional Stress

et al. 2021

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In modern life, the use of plant stress-protectors has taken on particular significance due to the wide distribution of neurosis-like and neurotic diseases caused by neuroendocrine-immune system imbalance. Special attention has been paid to the plants containing ecdysteroids, i.e., hormone-like bioactive substances with high adaptogenic activity. The article deals with the study of bioactivity of two plant extracts as Rhaponticum uniflorum (L.) DC. and Serratula centauroides L. with a high content of ecdysteroids and phenolic compounds. The models of acute and chronic emotional stress in white rats were used to estimate the stress-protective activity of R. uniflorum and S. centauroides extracts. Both extracts showed the stress-protective effect via inhibiting the development of signs induced by single and long-term effects of stress factors. In acute stress, the development of Selye's triad signs was less pronounced against the background of the plant remedies introduction. In chronic stress, the extracts prevented the development of anxiety-depressive syndrome. Besides, R. uniflorum and S. centauroides extracts banned the development of stress-induced injuries in the brain cortex and had a neuroprotective effect on ischemia against chronic stress. The stress-protective effects of both plant extracts were based on a decrease of hyperactivation of the central stress-promoting systems (sympathoadrenal, hypothalamic-pituitary-adrenal) due to their GABA-mimetic effects. Peripheral mechanisms were connected with the inhibition of free radical oxidation processes and with an increase in the endogenous antioxidant system activity. Thus, R. uniflorum and S. centauroides extracts have a high potential to increase non-specific body resistance against acute and chronic emotional stress effects.

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Section: Hplc-dad-esi-ms Profiles Of Rhaponticum Uniflorum and Serratula Centauroides Extractsmentioning

confidence: 63%

Section: Introductionmentioning

confidence: 99%

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Rhaponticum uniflorum and Serratula centauroides Extracts Attenuate Emotional Injury in Acute and Chronic Emotional Stress

et al. 2021

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“…Despite the widespread use of R. uniflorum as a medicinal plant, only few methods for the quantitative analysis of this plant material using liquid chromatography are known ( Table 3 ). To separate the main ecdysteroids of the herb and roots of R. uniflorum ( 28 , 25, 41 , 50 , 53 ), six variants of high performance liquid chromatography analysis on reversed-phase sorbents have been proposed, i.e., using the columns Ultrasphere ODS [ 7 ], Zorbax ODS [ 28 ], ProntoSIL 120-5 C18 [ 56 ], YMC-Pack C18 [ 57 ], GLC Mastro C18 [ 43 ], and Waters Acquity UPLC HSS T3 C18 [ 9 ] with 100–250-mm length [ 7 , 9 , 28 ] or 60-mm microcolumns [ 56 ]. Mixtures of methanol, acetonitrile, water, perchlorate buffer, and formic acid have been used as eluents to achieve separation in isocratic and gradient modes.…”

Section: Chromatographic Analysis Of R Uniflorummentioning

confidence: 99%

The Ethnopharmacological Uses, Metabolite Diversity, and Bioactivity of Rhaponticum uniflorum (Leuzea uniflora): A Comprehensive Review

Оленников

2022

Biomolecules

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Rhaponticum uniflorum (L.) DC. (syn. Leuzea uniflora (L.) Holub) is a plant species of the Compositae (Asteraceae) family that is widely used in Asian traditional medicines in China, Siberia, and Mongolia as an anti-inflammatory and stimulant remedy. Currently, R. uniflorum is of scientific interest to chemists, biologists, and pharmacologists, and this review includes information from the scientific literature from 1991 to 2022. The study of the chemodiversity of R. uniflorum revealed the presence of 225 compounds, including sesquiterpenes, ecdysteroids, triterpenes, sterols, thiophenes, hydroxycinnamates, flavonoids, lignans, nucleosides and vitamins, alkanes, fatty acids, and carbohydrates. The most studied groups of substances are phenolics (76 compounds) and triterpenoids (69 compounds). Information on the methods of chromatographic analysis of selected compounds, as well as on the quantitative content of some components in various organs of R. uniflorum, is summarized in this work. It has been shown that the extracts and some compounds of R. uniflorum have a wide range of biological activities, including anti-inflammatory, antitumor, immunostimulatory, anxiolytic, stress-protective, actoprotective, antihypoxic, anabolic, hepatoprotective, inhibition of PPARγ receptors, anti-atherosclerotic, and hypolipidemic. Published research on the metabolites and bioactivity of R. uniflorum does not include clinical studies of extracts and pure compounds; therefore, an accurate study of this traditional medicinal plant is needed.

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“…The presence of 20-hydroxyecdysone (ecdysterone) and 2-desoxy-20-hydroxyecdisone is characteristic for most species of Serratula [1,25]. 20-hydroxyecdysone is prevalent among ecdysteroids extracted from the plant; the total content of ecdysteroids in the herb of S. centauroides amounts to 1.42% [12]. In the folk medicine, particularly, in the practice of Buryat lamas, the decoction of the plant was used as antihaemorrhagic remedy [3]; the experiments have revealed that preparations from the plant have haemostatic and anabolic properties [15].…”

Section: Introductionmentioning

confidence: 99%

Phenolic Compounds of Serratula Centauroides and Anxiolytic Effect

Nikolaev¹

2019

FARMACIA

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The paper presents the results of the study of a dry extract of Serratula centauroides (Asteraceae) using a HPLC method and estimation of his anxiolytic activity. 17 phenolic substances and 15 respectively were identified in the herb in the roots. In the herb of the plant, there were revealed hyperoside and luteolin. The aerial part contains twice as much ecdysterone as the roots of S. centauroides. The experiments conducted on animals have shown that the S. centauroides dry extract at the doses comprised between 50 -150 mg/kg bw has a marked anxiolytic effect. S. centauroides extract increases the number of entries into the open branches of elevated plus maze (EPM) and prolongs a residence time in the open arms of EPM and light compartment of the dark/light chamber. The use of S. centauroides increases the number of punished water intakes in the Vogel conflict test. RezumatLucrarea prezintă rezultatele studiului efectuat asupra extractului uscat obținut de la Serratula centauroides (Asteraceae) prin metoda HPLC și estimarea activității anxiolitice a acestuia. Au fost identificate 17 substanțe fenolice în partea aeriană a plantei și 15 substanțe fenolice în rădăcini. În partea aeriană a plantei au fost descoperite hiperozida și luteolina. Partea aeriană conține de două ori mai mult ecdysteron decât rădăcinile de S. centauroides. Experimentele pe animale au arătat că extractul uscat administrat în doze cuprinse între 50 -150 mg/kgc are un efect anxiolitic marcant în testul labirintului și testul Vogel.

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Determination of ecdysteroids in Fornicium unflorum (L.) and Serratula centauroides (L.) raw materials by chromatography–UV spectrophotometry

Cited by 14 publications

References 8 publications

Rhaponticum uniflorum and Serratula centauroides Extracts Attenuate Emotional Injury in Acute and Chronic Emotional Stress

Rhaponticum uniflorum and Serratula centauroides Extracts Attenuate Emotional Injury in Acute and Chronic Emotional Stress

The Ethnopharmacological Uses, Metabolite Diversity, and Bioactivity of Rhaponticum uniflorum (Leuzea uniflora): A Comprehensive Review

Phenolic Compounds of Serratula Centauroides and Anxiolytic Effect

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