Determination of glutamine and serine in rat cerebrospinal fluid using capillary electrochromatography with a modified photopolymerized sol–gel monolithic column

Kato, Masaru; Jin, HongMei; Sakai‐Kato, Kumiko; Toyo’oka, Toshimasa; Dulay, Maria T.; Zare, Richard N.

doi:10.1016/s0021-9673(03)00451-5

Cited by 28 publications

(14 citation statements)

References 39 publications

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“…71 Os detectores baseados em fluorescência induzida por laser apresentam detectabilidade alta, no entanto, na maioria das aplicações são necessárias etapas de derivatização dos analitos para se obterem espécies fluorescentes. 72 O acoplamento CEC-MS apresenta algumas vantagens frente aos demais sistemas de detecção, entre elas maior seletividade, possibilidade de fornecer informações estruturais e a massa molar dos compostos e a identificação de amostras complexas, mesmo quando há sobreposição de picos.…”

Section: Detecção E Acoplamento Cec-msunclassified

Eletrocromatografia capilar: contextualização, estado da arte e perspectivas

Segato¹,

Silva²,

Jardim³

2009

Quím. Nova

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ElEtrocromatografia capilar: contExtualização, Estado da artE E pErspEctivas milena pinotti segato, césar ricardo silva e isabel cristina sales fontes Jardim* Instituto de Química, Universidade Estadual de Campinas, CP 6154, 13084-971 Campinas -SP, BrasilRecebido em 25/3/08; aceito em 21/8/08; publiado na web em 26/1/09 CAPILLARY ELECTROCHROMATOGRAPHY: CONTEXTUALIZATION, STATE-OF-THE-ART AND PERSPECTIVES. Capillary electrochromatography (CEC) is a separation technique in which the mobile phase flow is based on the application of a voltage across a packed capillary, which generates an electroosmotic flow that transports the analytes along the capillary toward the detector. As it combines the separation mechanisms of high-performance liquid chromatography (HPLC) and of capillary electrophoresis (CE), CEC can be considered a hybrid of HPLC and CE. This review presents some fundamental aspects of CEC and is focused on the instrumental advances of the technique, such as column technology, operation modes and detection systems, presenting recent papers on these topics and some applications and perspectives about CEC.

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Section: Detecção E Acoplamento Cec-msunclassified

Eletrocromatografia capilar: contextualização, estado da arte e perspectivas

Segato¹,

Silva²,

Jardim³

2009

Quím. Nova

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“…The method was used to determine histamine and its methylated metabolite in human urine samples using a fluorescence detector. Hybrid silica/polymeric monolithic columns were tested by Kato et al [16] for the CEC separation of NBD-derivatized amino acids. They prepared the stationaryiphase by photopolymerization of methacryloxypropyltrimethoxysilane (MPTMS).…”

Section: Amino Acids and Biogenic Aminesmentioning

confidence: 99%

Recent applications in capillary electrochromatography

Eeltink

Kok

2006

Electrophoresis

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In this review research papers on the application of CEC are summarized that have been published between May 2003 and May 2005. First, a short overview is given of trends and developments in CEC that may increase the applicability of the separation technique. Next, application-oriented research using CEC is described in biochemical studies, including proteomics and genomics, in the analysis of food and natural products, and in pharmaceutical, industrial, and environmental analysis.

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“…As a medium used in the microfluidic device monolithic column electrochromatograph (EC), PAA gel is a classical stationary phase for separation of proteins, enzyme and AAs [17,18]. Its application and preparation in general gel chromatography is well developed.…”

Section: The Characteristic Of the β-Cd-bonded Paa Gel Microchipmentioning

confidence: 99%

“…Noticeably, Tian et al [18,19] have done much work in the exploring the application of gel monolithic column in life sciences for capillary or microchip electrochromatography. Based on the widely adoption of gel monolithic column in contemporary analytical science, many complicated DNA and protein have been well separated in gel monolithic column for electrochromatograohy [17][18][19]. On the other hand, the chiral separation of gel monolithic column for electrochromatography has been focused on recently.…”

Section: Introductionmentioning

confidence: 99%