Determination of heparin levels in blood with activated partial thromboplastin time by a piezoelectric quartz crystal sensor

Cheng, Tzong Jih; Lin, Tsung-Han; Wu, Tsui Hsun; Chang, Hsien Chang

doi:10.1016/s0003-2670(00)01346-5

Cited by 59 publications

(11 citation statements)

References 17 publications

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“…The QCM biosensor has turned out to be a very useful alternative for determination of surface induced blood coagulation, since fibrin formation can be detected in situ and in real time on the electrode surface [16,17]. The classical QCM technique has been previously used to determine the concentration of fibrinogen [18,19], activity of blood coagulation factors [20], and coagulation time [21-23] with the advantages of real time output, label-free and high sensitivity. The frequency shifts give information about the general clotting kinetics, such as time of onset and fibrin deposition rate.…”

Section: Introductionmentioning

confidence: 99%

Detection of Fibrinogen and Coagulation Factor VIII in Plasma by a Quartz Crystal Microbalance Biosensor

Chen

Ling

2013

Sensors

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A quartz crystal microbalance (QCM) biosensor with nanogram sensitivity has been constructed through a reasonable designing and biological processing of the piezoelectric quartz crystals. Due to its highly sensitivity, real time detection and low cost, the proposed QCM biosensor has a promising potential in blood coagulation research. In the current study, the QCM biosensor was used to determine the activated partial thromboplastin time (APTT) for 120 anticoagulated plasma specimens. A good linear relationship was found in a double-logarithmic plot of APTT versus fibrinogen concentration in the range of 1.58–6.30 g/L. For factor VIII, the detection range by the QCM biosensor is 0.0185–0.111 mg/L. The QCM biosensor results were compared with those obtained by commercial optical coagulometry and a good agreement (correlation coefficient is 0.949 for fibrinogen, and 0.948 for factor VIII) was reached. Furthermore, the QCM determination can be completed within 10 min. Our study suggested that the proposed QCM biosensor could provide for more convenient and time saving operations, which may be useful in clinical situations for rapid monitoring of anticoagulant therapy using small volume (20 μL) plasma specimens.

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Section: Introductionmentioning

confidence: 99%

Detection of Fibrinogen and Coagulation Factor VIII in Plasma by a Quartz Crystal Microbalance Biosensor

Chen

Ling

2013

Sensors

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“…It has been shown in studies that the vibrational frequency of the crystal sensor changes the viscosity, density, and other characteristics of liquids [3][4][5][6]. Over the years, sensor applications on QCM have gained momentum in biomedical and biochemical fields and various sensor materials and sensor coatings have been utilized to detect various chemicals in liquid and gaseous media [7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Determination of blood glucose parameter from human blood serum by using a quartz crystal microbalance sensor coated with phthalocyanines compounds

et al. 2020

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Determining the blood glucose level is important for the prevention and treatment of diabetes mellitus. We developed a sensor system using Quartz Crystal Microbalance (QCM) to determine the blood glucose level from human blood serum. This study consists of two experimental stages: artificial glucose/pure water solution tests and human blood serum tests. In the first stage of the study, the QCM sensor with the highest performance was identified using artificial glucose solution concentrations. In the second stage of the study, human blood serum measurements were performed using QCM to determine blood glucose levels. QCM sensors were coated with phthalocyanines (Pcs) by jet spray method. The blood glucose values of 96 volunteers, which ranged from 71 mg/dL to 329 mg/dL, were recorded. As a result of the study, human glucose values were determined with an average error of 3.25%.

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“…[20,21] However, the result of these methods may not be sufficiently reliable, and the problems like high cost, time-consuming, or amenable for clinical settings also existed. Fluorescent assays have been proved to be an efficient and sensitive method for heparin detection or quantification.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of a novel water-soluble conjugated polyelectrolyte based on polycyclopentadithiophene backbone and its application for heparin detection

Zhu

Guo

et al. 2014

Designed Monomers and Polymers

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Synthesis of a novel water-soluble conjugated polyelectrolyte based on polycyclopentadithiophene backbone and its application for heparin detection, Designed Monomers and Polymers, 17:7, 624-628,The novel water-soluble conjugated polyelectrolyte containing a new kind of backbone poly [(4,4-bis(3ʹ-N,N,N-trimethylammonium) propyl) cyclopentadithiophene dichloride] (PBTPC-Cl) is synthesized and characterized. The PBTPC-Cl is readily soluble in polar solvents and is applied as a sensitive and selective fluorescent probe for heparin detection in aqueous solution. Upon adding heparin into the aqueous solution of polymer, PBTPC-Cl could bind heparin via electrostatic interactions and results in the quenching of emission peaks. Quantitative analysis of heparin shows high selectivity and good linear relationship with a detection limit to 0.05 μM in HEPES aqueous buffer.

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Determination of heparin levels in blood with activated partial thromboplastin time by a piezoelectric quartz crystal sensor

Cited by 59 publications

References 17 publications

Detection of Fibrinogen and Coagulation Factor VIII in Plasma by a Quartz Crystal Microbalance Biosensor

Detection of Fibrinogen and Coagulation Factor VIII in Plasma by a Quartz Crystal Microbalance Biosensor

Determination of blood glucose parameter from human blood serum by using a quartz crystal microbalance sensor coated with phthalocyanines compounds

Synthesis of a novel water-soluble conjugated polyelectrolyte based on polycyclopentadithiophene backbone and its application for heparin detection

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