Determination of imidapril and imidaprilat in human plasma by high-performance liquid chromatography–electrospray ionization tandem mass spectrometry

Angiotensin II directly induces muscle protein catabolism through the ubiquitin–proteasome proteolytic pathway and may play a role in cancer cachexia

“…The results presented in Figure 1A show that Ang I significantly stimulated protein degradation, with a parabolic dose -response curve, as previously observed with PIF (Whitehouse and Tisdale, 2003), and with a maximal effect at concentrations between 0.05 and 0.1 mM. The effect was attenuated by coincubation with imidaprilat (50 mM), the active metabolite of the highly lipophilic ACE inhibitor imidapril (Vitort) (Mabuchi et al, 1999). These results suggest that Ang I stimulates protein degradation in muscle through the conversion to Ang II.…”

“…Ang I also mediates the same effects through conversion to Ang II by ACE, since the ACE inhibitor imidaprilat, the active metabolite of imidapril, in which an ethyl ester group is hydrolysed (Mabuchi et al, 1999), attenuates the action of Ang I. There is also evidence to suggest that formation of Ang II may contribute to the loss of body tissue in mice bearing the MAC16 tumour, since imidapril attenuated the development of weight loss in this model.…”

Allosteric kinetics of human carboxylesterase 1: Species differences and interindividual variability

“…Imidaprilat formation from imidapril was determined according to the method of Mabuchi et al17 with slight modifications. A typical incubation mixture (200 µL of total volume) contained the enzyme source and 100 mM tris(hydroxymethyl)aminomethane‐HCl buffer (pH 7.4).…”

Different Effects of Imidapril and Enalapril on Aminopeptidase P Activity in the Mouse Trachea

“…Imidapril, imidaprilat, enalapril and enalaprilat, which were retained in the cartridge, were eluted with 1 ml of methanol into a disposable glass tube, and evaporated to dryness at 40°C under a stream of nitrogen. The residue was dissolved in 100 μl of internal standard solution (0.1 μg/ml mobile phase), and a 10-μl aliquot was analyzed by the HPLC-ESI-MS-MS system as described previously (10). …”