Determination of immunoglobulin A concentrations in the serum and cerebrospinal fluid of dogs: An estimation of its diagnostic value in canine steroid-responsive meningitis–arteritis

Maiolini, Arianna; Carlson, Regina; Schwartz, Michael F.; Gandini, Gualtiero; Tipold, Andrea

doi:10.1016/j.tvjl.2010.12.018

Cited by 35 publications

(61 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previously established clinical criteria (Bathen-Noethen at al., 2009; Maiolini et al, 2011) were used to identify clinical cases of SRMA, including results of serum and CSF analyses. Serum and CSF samples were collected from dogs acutely affected with SRMA (n=30), a population of SRMA affected dogs treated with anti-inflammatory corticosteroids (n=30), and healthy control dogs (n=8).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Extracellular hsp70 release in Canine Steroid Responsive Meningitis-Arteritis

Moore

Kim

Maiolini

et al. 2012

Veterinary Immunology and Immunopathology

Self Cite

View full text Add to dashboard Cite

The role of extracellular 70 kDa heat shock protein 70 (ehsp70) in central nervous system inflammation is vastly understudied, despite evidence supporting the ability to drive a pro-inflammatory state. We investigated the presence of ehsp70 in cerebrospinal fluid (CSF) and serum of dogs with Steroid Responsive Meningitis-Arteritis (SRMA), with the hypothesis that an ehsp70 response would occur, and might play a role in the pathogenesis of this disease. Samples from 30 dogs acutely affected with SRMA, and 30 dogs treated with corticosteroids and currently in clinical remission from SRMA were compared with normal dogs. Serum and CSF concentrations of ehsp70 were quantified using an enzyme-linked immunosorbent assay. An ehsp70 response occurred in the CSF of dogs with SRMA and this response was attenuated by corticosteroid treatment. There was no correlation between serum and CSF concentrations of ehsp70, supporting local production and release of ehsp70 and not simply leakage from serum. Dogs with SRMA thus represent a powerful spontaneous model by which to study the role of ehsp70 in CNS inflammation.

show abstract

Section: Methodsmentioning

confidence: 99%

“…Concentrations of CSF and serum IgA for 27 of the acutely affected dogs had been previously measured and published (Bathen-Noethen, 2008; Maiolini et al, 2011). For samples which this information was available, these values were correlated with ehsp70 concentrations in CSF and serum.…”

Section: Methodsmentioning

confidence: 99%

Extracellular hsp70 release in Canine Steroid Responsive Meningitis-Arteritis

Moore

Kim

Maiolini

et al. 2012

Veterinary Immunology and Immunopathology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Several studies [13–17] confirmed an immune-mediated disease. Furthermore, high concentrations of immunoglobulin A (IgA) have been found both intrathecally and systemically in dogs affected with SRMA [13, 18]. …”

Section: Introductionmentioning

confidence: 99%

“…Up to now, no infectious agents eliciting the disease have been consistently detected [4, 6, 7, 10–12]. Several studies [13–17] confirmed an immune-mediated disease. Furthermore, high concentrations of immunoglobulin A (IgA) have been found both intrathecally and systemically in dogs affected with SRMA [13, 18].…”

Section: Introductionmentioning

confidence: 99%

Th17-skewed immune response and cluster of differentiation 40 ligand expression in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis

Freundt-Revilla

Maiolini

Carlson

et al. 2017

J Neuroinflammation

Self Cite

View full text Add to dashboard Cite

BackgroundSteroid-responsive meningitis-arteritis (SRMA) is an immune-mediated disorder characterized by neutrophilic pleocytosis and an arteritis particularly in the cervical leptomeninges. Previous studies of the disease have shown increased levels of IL-6 and TGF-ß1 in cerebrospinal fluid (CSF). In the presence of these cytokines, naive CD4+ cells differentiate into Th17 lymphocytes which synthesize interleukin 17 (IL-17). It has been shown that IL-17 plays an active role in autoimmune diseases, it induces and mediates inflammatory responses and has an important role in recruitment of neutrophils. The hypothesis of a Th17-skewed immune response in SRMA should be supported by evaluating IL-17 and CD40L, inducing the vasculitis.MethodsAn enzyme-linked immunosorbent assay (ELISA) was performed to measure IL-17 and CD40L in serum and CSF from a total of 79 dogs. Measurements of patients suffering from SRMA in the acute state (SRMA A) were compared with levels of patients under treatment with steroids (SRMA T), recurrence of the disease (SRMA R), other neurological disorders, and healthy dogs, using the two-part test. Additionally, secretion of IL-17 and interferon gamma (IFN-γ) from the peripheral blood mononuclear cells (PBMCs) was confirmed by an enzyme-linked immunospot (ELISpot) assay.ResultsSignificant higher levels of IL-17 were found in CSF of dogs with SRMA A compared with SRMA T, other neurological disorders and healthy dogs (p < 0.0001). In addition, levels of CD40L in CSF in dogs with SRMA A and SRMA R were significantly higher than in those with SRMA T (p = 0.0004) and healthy controls (p = 0.014). Furthermore, CSF concentrations of IL-17 and CD40L showed a strong positive correlation among each other (rSpear = 0.6601; p < 0.0001) and with the degree of pleocytosis (rSpear = 0.8842; p < 0.0001 and rSpear = 0.6649; p < 0.0001, respectively). IL-17 synthesis from PBMCs in SRMA patients was confirmed; however, IL-17 is mainly intrathecally produced.ConclusionsThese results imply that Th17 cells are inducing the autoimmune response in SRMA and are involved in the severe neutrophilic pleocytosis and disruption of the blood-brain barrier (BBB). CD-40L intrathecal synthesis might be involved in the striking vasculitis. The investigation of the role of IL-17 in SRMA might elucidate important pathomechanism and open new therapeutic strategies.

show abstract

“…The lack of identifiable infectious organisms, response to treatment with steroids, high concentrations of IgA in the serum and CSF and increased interleukin 6 (IL-6) and transforming growth factor beta 1 (TGF-β 1 ) in CSF support this statement (Maiolini and others 2012, Maiolini and others 2013, Rose and Harcourt-Brown 2013, Biedermann and others 2016). IL-6 and TGF-β 1 cause CD4 progenitors to differentiate towards a T helper (Th 17), and a recent study demonstrated that Th17 cells induce the autoimmune response in SRMA and are involved in the development of the severe neutrophilic pleocytosis and disruption of the blood–brain barrier (Maiolini and others 2013, Freundt-Revilla and others 2017).…”

Section: Discussionmentioning

confidence: 99%

Intracerebral haemorrhage in a dog with steroid‐responsive meningitis arteritis

Brocal

José-López

Hammond

et al. 2017

Vet Record Case Reports

View full text Add to dashboard Cite

A one‐year and six‐month‐old female neutered Boxer dog was presented with a four‐day history of pyrexia, lethargy and neck pain. An intracerebral haemorrhage and a mediastinal mass were identified. Cerebrospinal fluid analysis revealed severe neutrophilic pleocytosis, and steroid‐responsive meningitis arteritis (SRMA) was suspected. A significant improvement was observed with immunosuppressive steroid therapy and subsequent imaging revealed a reduction in size of the intracerebral haemorrhage and disappearance of the mediastinal mass. SRMA is a systemic disease with potential involvement of multiple organs. Intracerebral haemorrhage can occur secondary to SRMA and can have a successful outcome.

show abstract

Determination of immunoglobulin A concentrations in the serum and cerebrospinal fluid of dogs: An estimation of its diagnostic value in canine steroid-responsive meningitis–arteritis

Cited by 35 publications

References 20 publications

Extracellular hsp70 release in Canine Steroid Responsive Meningitis-Arteritis

Extracellular hsp70 release in Canine Steroid Responsive Meningitis-Arteritis

Th17-skewed immune response and cluster of differentiation 40 ligand expression in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis

Intracerebral haemorrhage in a dog with steroid‐responsive meningitis arteritis

Contact Info

Product

Resources

About