Determination of multi-mycotoxins in cereals and of total fumonisins in maize products using isotope labeled internal standard and liquid chromatography/tandem mass spectrometry with positive ionization

Andrade, Patrícia Diniz; Dantas, Rebecca Rodrigues; Moura-Alves, Tatiana Loureiro da Silva de; Caldas, Eloísa Dutra

doi:10.1016/j.chroma.2017.02.027

Cited by 63 publications

(37 citation statements)

References 29 publications

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“…While reviewing available literature, only a few direct pieces of guidance concerning the acceptability of standard solution concentration variation during storage were noted. According to Andrade et al, stock standard solutions of AFLs, ochratoxin A (OTA), zearalenone (ZEA), deoxynivalenol (DON), 3-acetyl-and 15-acetyl deoxynivalenol (3-AcDON and 15-AcDON) and citreoviridin (CTV) were considered valid after a storage period if their UV-spectrophotometric check resulted in a maximum of 3% difference with the previous check of freshly prepared solutions [10]. Some other authors also reported 2%-3% variation as suitable [11,12], while the majority did not address this point.…”

Section: Stability Of Diluted Individual Stock Standard Solutionsmentioning

confidence: 99%

Stability of Mycotoxins in Individual Stock and Multi-Analyte Standard Solutions

Kiseleva¹,

Chalyy²,

Седова³

et al. 2020

Toxins

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Standard solutions of mycotoxins prepared in RP HPLC solvents from neat standards are usually used for analytical method development. Multi-mycotoxin HPLC-MS/MS methods necessitate stability estimation for the wide spectrum of fungal metabolites. The stability of individual diluted stock standard solutions of mycotoxins in RP-HPLC solvents and multi-analyte HPLC-MS/MS calibrants was evaluated under standard storage and analysis conditions. Individual stock standard solutions of aflatoxins, sterigmatocystin, A-and B-trichothecenes, zearalenone and its analogues, ochratoxin A, fumonisins, Alternaria toxins, enniatins and beauvericin, moniliformin, citrinin, mycophenolic, cyclopiazonic acids and citreoviridin were prepared in RP-HPLC solvents and stored at −18 • C for 14 months. UV-spectroscopy was utilized to monitor the stability of analytes, excluding fumonisins. The gradual degradation of α-, β-zearalenol and α-, β-zearalanol in acetonitrile was detected. Aflatoxins and sterigmatocystin, zearalenone, Alternaria toxins, enniatins and beauvericin, citrinin, mycophenolic, cyclopiazonic acids and citreoviridin can be referred to as stable. The concentration of the majority of trichothecenes should be monitored. Diluted multi-mycotoxin standard in water/methanol (50/50 v/v) solutions acidified with 0.1% formic acid proved to be stable in silanized glass at 23 • C exposed to light for at least 75 h (CV ≤ 10%). An unexpected manifestation of MS/MS signal suppression/enhancement was discovered in the course of multi-mycotoxin standard solution stability evaluation. Key Contribution:The stability of the wide spectrum of mycotoxins in individual standard solutions in RP-HPLC solvents and multi-analyte HPLC-MS/MS calibrants under standard storage and analysis conditions is discussed basing on experimental and literature data.

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Section: Stability Of Diluted Individual Stock Standard Solutionsmentioning

confidence: 99%

Stability of Mycotoxins in Individual Stock and Multi-Analyte Standard Solutions

Kiseleva¹,

Chalyy²,

Седова³

et al. 2020

Toxins

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“…In the case of positional isomers, proper quantification of 3Ac-DON and 15Ac-DON cannot be achieved through the difference of the product ion in MRM mode. In positive mode (339 m / z ) the most abundant parent ion was the same for both analytes, but different product ions were chosen (339/231.2 and 339/279.1 for 3Ac-DON and 339/321.2 and 339/261.2 for 15Ac-DON) [ 27 , 28 ]. However, other studies show higher ionisation in positive mode with NH4 + adducts for 3Ac-DON and 15-AcDON [ 13 , 23 ] or in negative modes [ 14 , 16 ].…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, the choice of ACN shortened retention time for all analytes, as well as the time of analysis. To date a lot of studies have described the chromatographic separation of DON, its modified mycotoxins, and other type B Trichothecenes in different biological matrixes [ 13 , 14 , 16 , 18 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ]. Nevertheless, most of the authors did not achieve baseline separation of acetylated forms of DON.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous Determination of Deoxynivalenol, Its Modified Forms, Nivalenol and Fusarenone-X in Feedstuffs by the Liquid Chromatography–Tandem Mass Spectrometry Method

Panasiuk

Jedziniak

Pietruszka

et al. 2020

Toxins

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A liquid chromatography-tandem mass spectrometry method was developed for simultaneous determination of deoxynivalenol (DON), 3-acetyldeoxynivalenol (3Ac-DON), 15-acetyldeoxynivalenol (15Ac-DON), DON-3-glucoside (DON-3Glc) nivalenol and fusarenone-X in feedstuffs. Different techniques of sample preparation were tested: solid-liquid-extraction, QuEChERS, solid phase extraction with OASIS HLB columns or immunoaffinity columns and a Mycosep 225 Trich column. None of the six immunoaffinity columns tested showed cross-reactivity to all of the mycotoxins. Surprisingly, the results show that if the immunoaffinity columns bound 3Ac-DON, then they did not bind 15Ac-DON. The most efficient sample preparation was achieved with a Mycosep 225 Trich column clean-up. The chromatography was optimised to obtain full separation of all analytes (including 3Ac-DON and 15Ac-DON isomeric form). The validation results show the relative standard deviations for repeatability and reproducibility varied from 4% to 24%. The apparent recovery ranged between 92% and 97%, and the limit of quantification described a 1.30 to 50 µg/kg range. The method trueness was satisfactory, as assessed by a proficiency test and analysis of reference material. A total of 99 feed samples were analysed by the developed method, revealing the presence of DON and DON-3Glc in 85% and 86% of examined animal feeds, respectively at concentrations between 1.70 and 1709 µg/kg. The ratios DON-3Glc to DON in the surveyed feedstuffs were from a low of 3% to high of 59%.

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“…At present, isotope‐labelled internal standards have been mainly used in the detection of mycotoxins in foods and cereals . Andrade et al . developed an isotope‐labelled internal standard and liquid chromatography/tandem mass spectrometry method for multimycotoxins determination in cereals and for total fumonisins in maize products.…”

Section: Introductionmentioning

confidence: 99%

“…[31] At present, isotope-labelled internal standards have been mainly used in the detection of mycotoxins in foods and cereals. [32,33] Andrade et al [34] developed an isotope-labelled internal standard and liquid chromatography/tandem mass spectrometry method for multimycotoxins determination in cereals and for total fumonisins in maize products. Gao et al [35] proposed an isotope internal standard-based LC-MS/MS method for the simultaneous determination of five mycotoxins in various grains and their products.…”

Section: Introductionmentioning

confidence: 99%

Multiclass mycotoxins in lotus seeds analysed by an isotope-labelled internal standard-based UPLC-MS/MS

Huang

Kong

Wang

et al. 2018

Journal of Pharmacy and Pharmacology

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Determination of multi-mycotoxins in cereals and of total fumonisins in maize products using isotope labeled internal standard and liquid chromatography/tandem mass spectrometry with positive ionization

Cited by 63 publications

References 29 publications

Stability of Mycotoxins in Individual Stock and Multi-Analyte Standard Solutions

Stability of Mycotoxins in Individual Stock and Multi-Analyte Standard Solutions

Simultaneous Determination of Deoxynivalenol, Its Modified Forms, Nivalenol and Fusarenone-X in Feedstuffs by the Liquid Chromatography–Tandem Mass Spectrometry Method

Multiclass mycotoxins in lotus seeds analysed by an isotope-labelled internal standard-based UPLC-MS/MS

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